| Findlay Street Records Ltd | Information & communication | 2025 |
| FIP Support UK Ltd | Professional & scientific | 2023 |
| FLY Scotland Limited | Administrative & support | 2024 |
| Flying KEG Limited | Hospitality | 2011 |
| Focus Group (Investments) Limited | Professional & scientific | 2008 |
| Focus Offshore Logistics Limited | Professional & scientific | 2007 |
| Focus OIL and GAS Limited | Mining & quarrying | 2008 |
| Focus Subsea Limited | Professional & scientific | 2006 |
| Ford Properties (Fife) Ltd | Real estate | 2022 |
| Forthsight Ltd | Information & communication | 2018 |
| Forthwind Limited | Energy | 2014 |
| Fried Guys Ltd | Hospitality | 2019 |
| Fundraise Arcade Limited | Arts & recreation | 2024 |
| G&G Mcguff Properties Ltd | Real estate | 2026 |
| G.Andrews NDT Services Ltd | Professional & scientific | 2018 |
| G2E Services Ltd | Energy | 2019 |
| Garviesystems Ltd | Information & communication | 2019 |
| Gemlock Limited | Mining & quarrying | 2015 |
| Gillian Normand Consultancy Ltd | Financial services | 2018 |
| Giveawaycouk Ltd | Wholesale & retail | 2021 |
| Golf & Whisky Getaway Breaks Limited | Hospitality | 2025 |
| Grain & Sustain Ltd | Wholesale & retail | 2023 |
| Grange Park Leisure Ltd. | Arts & recreation | 1998 |
| Gray & Gray Properties Limited | Real estate | 2015 |
| Griffin Inspections and Access Ltd | Professional & scientific | 2022 |
| GW Mechanical Engineering Ltd | Manufacturing | 2025 |
| Haar Pilates Ltd | Arts & recreation | 2025 |
| Haircomb @ Salon Denis Limited | Other services | 2003 |
| Hallcroft Farms Ltd | Agriculture & fishing | 2022 |
| HAM Investments Ltd | Real estate | 2019 |
| HAN Johnston Limited | Hospitality | 2014 |
| Happy Tasty Dumplings Ltd | Hospitality | 2025 |
| I.M.R Roughcasting Limited | Construction | 2022 |
| Inchcolm Developments Ltd | Construction | 2017 |
| Inside & OUT Digital Ltd. | Information & communication | 2007 |
| Inveraegan Marine Services Ltd. | Professional & scientific | 2017 |
| J C M Beauty Studio Ltd | Other services | 2021 |
| James Moffat & Sons Limited | Transport & logistics | 1983 |
| JKP Engineering Limited | Professional & scientific | 2012 |
| JMS Taxis Limited | Extraterritorial | 2013 |
| JO JO Co. Candles Ltd | Wholesale & retail | 2021 |
| Johnston Coaching Limited | Other services | 2024 |
| Jykel Ltd | Professional & scientific | 2018 |
| K&G Enterprises Ltd | Hospitality | 2018 |
| Kairosynthesis Ltd | Professional & scientific | 2026 |
| KAM Aesthetics and Beauty Clinic Ltd | Other services | 2022 |
| KDM Contracts Ltd | Construction | 2021 |
| KEO Walker Racing Ltd | Arts & recreation | 2025 |
| Kevlar Events Ltd | Hospitality | 2020 |
| Kinetic Technical Consulting Limited | Professional & scientific | 2021 |
| Kingdom Commercial Cleaning Ltd | Administrative & support | 2024 |
| Kinghorn Community Land Association | Health & social care | 2005 |
| Kinghorn Solutions Ltd | Wholesale & retail | 2022 |
| Kingswood Hotel Scotland Ltd | Hospitality | 2022 |
| Kirkbank Ltd | Administrative & support | 2018 |
| KSH Consulting Limited | Real estate | 2024 |
| KY GAS Fife Limited | Construction | 2017 |
| Landscaping Your Life Ltd | Professional & scientific | 2013 |
| Last Looks Software Limited | Information & communication | 2025 |
| LD Property (Fife) Ltd | Real estate | 2023 |
| LDL GAS Ltd | Construction | 2011 |
| LHM Ltd | Wholesale & retail | 2026 |
| Lillycyber GRC Consulting Limited | Information & communication | 2026 |
| Little Living Room Ltd | Information & communication | 2020 |
| LIZ Melville Cleaning Services Limited | Households | 2013 |
| Lorna Moffat Coaching Limited | Administrative & support | 2025 |
| LTM Taxis | | 2004 |
| Macdonald North Limited | Administrative & support | 2004 |
| Magicol Holdings Limited | Administrative & support | 2020 |
| Maitland Motors Ltd | Wholesale & retail | 2025 |
| Malmer Limited | Information & communication | 2026 |
| Mayanna Properties Limited | Real estate | 2024 |
| Maybell Services Ltd | Professional & scientific | 2023 |
| MC Steading Ltd | Agriculture & fishing | 2019 |
| Mckenzie Commercial Services Limited | Professional & scientific | 1997 |
| MD Plant Electrical Ltd | Construction | 2023 |
| Memchats Cakes Ltd | Manufacturing | 2025 |
| Memchats Investments Ltd | Real estate | 2025 |
| Merimac Events Ltd | Professional & scientific | 2026 |
| Mitchcs Ltd | Transport & logistics | 2025 |
| Moncrieff Security Solutions Limited | Administrative & support | 2014 |
| Mossie Quality Solutions Limited | Professional & scientific | 2025 |
| MTD Scotland Ltd | Professional & scientific | 2017 |
| Mthungwa Healthcare Limited | Health & social care | 2022 |
| Murrayfield & Co Ltd | Professional & scientific | 2016 |
| MUX Design Ltd | Information & communication | 2023 |
| Nationwide GAS Care Limited | Manufacturing | 2000 |
| Natsmiles (Property) Limited | Health & social care | 2010 |
| Natsmiles Limited | Health & social care | 2009 |
| Niaroo Business Services Limited | Professional & scientific | 2019 |
| Nine TEN Ventures Ltd | Financial services | 2026 |
| One One Four Bistro Ltd | Hospitality | 2022 |
| One One Four Trading Ltd | Wholesale & retail | 2020 |
| Onsight Solutions Ltd | Administrative & support | 2015 |
| P M Dental Limited | Health & social care | 2006 |
| Pama Holdings Limited | Real estate | 2001 |
| Pama Solutions Ltd | Information & communication | 2023 |
| PDT Manufacturing Ltd | Professional & scientific | 2008 |
| Pebble Pictures Ltd | Information & communication | 2024 |
| Pettycur Consulting Ltd | Professional & scientific | 2017 |