| A&F Consultancy Limited | Professional & scientific | 2016 |
| ABC NDT Ltd | Professional & scientific | 2025 |
| Aberdee Limited | Professional & scientific | 2025 |
| Aberdour Bistro Ltd | Hospitality | 2022 |
| Aberdour Psychology and Psychotherapy Services Limited | Health & social care | 2011 |
| Abhaile Interiors Ltd | Wholesale & retail | 2026 |
| Accountancy Fife Limited | Professional & scientific | 2005 |
| Active Woofers Wholesale Ltd | Wholesale & retail | 2021 |
| Adam Cartridges Ltd | Manufacturing | 2026 |
| Against the Grain Scotland Ltd | Administrative & support | 2024 |
| AJW Development & Consultancy Ltd | Real estate | 2025 |
| Alison M Cooper Ltd | Health & social care | 2022 |
| AM Creels Limited | Agriculture & fishing | 2024 |
| Amperxander Ltd | Professional & scientific | 2023 |
| Amulree Edinburgh Ltd | Hospitality | 2023 |
| Anchorage Panoramics Ltd | Professional & scientific | 2011 |
| ARB Wind Limited | Professional & scientific | 2018 |
| Arbor Vitae Arboriculture Ltd | Professional & scientific | 2011 |
| Arrankirk Limited | Transport & logistics | 2002 |
| ASA Farming Limited | Agriculture & fishing | 2009 |
| AT Network Support Services Limited | Administrative & support | 2015 |
| B Hub Limited | Administrative & support | 2018 |
| B Malone Properties Ltd | Real estate | 2020 |
| B&L Bars Ltd | Hospitality | 2018 |
| B.D. Joinery Limited | Construction | 2024 |
| Bankhead Energy Limited | Energy | 2014 |
| Bay View Kinghorn Limited | Hospitality | 2022 |
| BDM Joinery Ltd | Construction | 2021 |
| Beacon Kids Network Ltd | Information & communication | 2026 |
| Benchmark Bespoke Ltd | Construction | 2026 |
| Bendameer Consulting Limited | Professional & scientific | 2003 |
| Big Green Market CIC | Wholesale & retail | 2024 |
| Black Sands Holdings Ltd | Households | 2023 |
| Black Sands Partnership LLP | | 2023 |
| Blake's Vintage and Collectables Limited | Wholesale & retail | 2011 |
| Blyth Marine Services Limited | Construction | 2018 |
| Borg Contractors Ltd | Wholesale & retail | 2015 |
| Borg Services Limited | Wholesale & retail | 2015 |
| Briggs (Overseas) Limited | Extraterritorial | 2001 |
| Briggs Commercial Limited | Administrative & support | 1972 |
| Briggs Environmental Services Limited | Water & waste | 1988 |
| Briggs Marine Contractors Limited | Manufacturing | 1988 |
| Briggs Marine Environmental Services Limited | Wholesale & retail | 2002 |
| BRS Builders Limited | Construction | 2009 |
| Build Force Limited | Construction | 2017 |
| Burdiehouse Limited | Mining & quarrying | 2014 |
| Burnside Pet Retreat Limited | Other services | 2024 |
| Burntisland Community Development Trust | Extraterritorial | 2016 |
| Burntisland Golf Company, Limited | Arts & recreation | 1895 |
| Burntisland Harbour Access Trust Limited | Education | 2021 |
| Burntisland Recreation Company Limited | Arts & recreation | 1892 |
| Burntisland Sailing Club Limited | Arts & recreation | 2015 |
| BW Technical Limited | Professional & scientific | 2019 |
| C & W White Ltd | Hospitality | 2015 |
| Cafe Verde Ltd | Hospitality | 2024 |
| Café @ Number 16 Limited | Hospitality | 2025 |
| Caird 4 Technologies Ltd | Manufacturing | 2024 |
| Calath Investments Ltd | Households | 2024 |
| Caledonia Consultants Limited | Professional & scientific | 2025 |
| Camat Design Limited | Professional & scientific | 2017 |
| Cardforge Limited | Information & communication | 2025 |
| Carpentry Creations Ltd | Construction | 2024 |
| Chantelle Properties Limited | Real estate | 2001 |
| CK Industrial Services Ltd | Construction | 2019 |
| Cmacg Ltd | Professional & scientific | 2021 |
| Cornerstone Exchange LLP | | 2010 |
| Cornerstone Exchange NO. 2 LLP | | 2012 |
| Couston Consultancy Limited | Professional & scientific | 2019 |
| Couston Motor Company Ltd | Wholesale & retail | 2020 |
| D Leadbetter & SON (Shellfish) Ltd | Agriculture & fishing | 2018 |
| DC Four Ltd | Wholesale & retail | 2025 |
| DCC Rail Projects Ltd | Professional & scientific | 2022 |
| Digital Media Fusion Limited | Information & communication | 2025 |
| Dilitech Ltd | Information & communication | 2003 |
| Ditzel Limited | Information & communication | 2018 |
| Divinefingersconsult Publishing Ltd | Information & communication | 2026 |
| Donovan Naval Architecture Ltd | Professional & scientific | 2026 |
| DR. ZOE Roxburgh Speech and Language Therapy Ltd | Health & social care | 2024 |
| Drago Industries Ltd | Professional & scientific | 2016 |
| Dragon Leopard Wolf Limited | Real estate | 2021 |
| Dunearn Hill Soap Co. Ltd | Manufacturing | 2021 |
| D’Andria Holdings Ltd | Households | 2024 |
| EAC Couriers (Scotland) Limited | Transport & logistics | 2022 |
| Easter Bendameer Limited | Real estate | 2010 |
| EAT Balanced Limited | Manufacturing | 2010 |
| Econ Property Maintenance Limited | Construction | 2024 |
| Eden Knight Properties Ltd | Real estate | 2026 |
| Edenta Ltd | Health & social care | 2010 |
| Electro-Scot Transformers Limited | Manufacturing | 2008 |
| Esbee Holdings Ltd | Financial services | 2018 |
| Eudeielos Ltd | Financial services | 2026 |
| F.D.Young Photography Ltd | Professional & scientific | 2024 |
| Fess Star Ltd | Wholesale & retail | 2020 |
| FGP Partners Limited | Real estate | 2020 |
| Fife Hardware Ltd | Wholesale & retail | 2020 |
| Fife Historic Buildings Trust | Construction | 1997 |
| Fife Hotels Limited | Real estate | 2017 |
| Fife Hotels Trading (Aberdour) Limited | Hospitality | 2024 |
| Fife Hotels Trading Limited | Hospitality | 2020 |
| Fifty SIX Degrees North Limited | Transport & logistics | 2022 |