| East Riding Arborists Limited | Agriculture & fishing | 2021 |
| East Yorkshire E-Bikes Ltd | Wholesale & retail | 2025 |
| East Yorkshire Hemp Limited | Manufacturing | 2024 |
| East Yorkshire Retrofit Solutions Ltd | Wholesale & retail | 2021 |
| East Yorkshire Steel Fabrications Limited | Manufacturing | 2020 |
| Eastfield Garden Centre Limited | Wholesale & retail | 2003 |
| Eastfield Garden Centre Retail Limited | Wholesale & retail | 2023 |
| Eastfield Garden Centre Services Limited | Administrative & support | 2020 |
| Eastyorkshiresprayers Ltd | Construction | 2025 |
| Easyforce Limited | Arts & recreation | 2019 |
| Ebbfix Limited | Manufacturing | 1995 |
| Echo Media Group Ltd | Information & communication | 2020 |
| Eco Brickwork Contracts Ltd | Construction | 2025 |
| Eco Brickwork Limited | Construction | 2009 |
| Ecridley Ltd | Health & social care | 2017 |
| Edwards & George Electrical Contractors Ltd | Construction | 2023 |
| Edwardson Associates Limited | Professional & scientific | 2002 |
| Egina Ltd | Wholesale & retail | 2025 |
| EL Tyres Limited | Wholesale & retail | 2024 |
| Elevated Scaffolding Ltd | Construction | 2021 |
| Elim House Properties Ltd | Real estate | 2025 |
| Elite Fabrications & Maintenance Services Limited | Manufacturing | 1995 |
| Elizabeth's Limited | Wholesale & retail | 2002 |
| Elliott Hancocks Ltd | Real estate | 2023 |
| Ellis Holdings Bridlington Ltd | Financial services | 2017 |
| Ellis's Home Improvements Ltd | Construction | 2024 |
| ELM Bank Coastal Park Limited | Extraterritorial | 2023 |
| ELM Bank Leisure Limited | Extraterritorial | 2023 |
| EM (Bridlington) Limited | Hospitality | 2016 |
| EMI Cars Jacek Olszewski Limited | Manufacturing | 2014 |
| Emmerson and Co (Fishing) Limited | Agriculture & fishing | 2002 |
| Emswelding Ltd | Construction | 2018 |
| Esme Qadir Ltd | Wholesale & retail | 2019 |
| Estates Engineering Compliance Consultant Limited | Administrative & support | 2015 |
| European Hail Solutions Limited | Wholesale & retail | 2015 |
| Eurovision Logistics France Limited | Transport & logistics | 2020 |
| Eve's @ Pink Pigeon Wellbeing C.I.C. | Wholesale & retail | 2025 |
| Eventsbee.Co.Uk Ltd | Extraterritorial | 2021 |
| Everhaus Limited | Manufacturing | 2024 |
| Evolve LET Management Ltd | Real estate | 2026 |
| Evolve Roofing Ltd | Construction | 2021 |
| Evolve Solar Ltd | Construction | 2023 |
| Excellent Cafe Ltd | Hospitality | 2025 |
| Excellent IS Excellence Ltd | Hospitality | 2025 |
| Explore Consultancy Limited | Professional & scientific | 2025 |
| Express Coach Repairs Limited | Wholesale & retail | 1984 |
| Extraction Search Limited | Professional & scientific | 2013 |
| F.C. Stubbings & Sons Limited | Agriculture & fishing | 1957 |
| Fagin's Hock Shop Limited | Arts & recreation | 2025 |
| Fairways Part Worn Tyres Limited | Wholesale & retail | 2019 |
| Family Flavours Catering Ltd | Hospitality | 2025 |
| Farmhouse Coffee and Deli Limited | Hospitality | 2025 |
| Fart Ltd | Professional & scientific | 2006 |
| Fbgc Limited | Wholesale & retail | 2017 |
| Feeley Construction Ltd | Construction | 2017 |
| Felicitas Management Limited | Extraterritorial | 2021 |
| Fencit Limited | Administrative & support | 2002 |
| Fergus Lyon Land and Sea Limited | Professional & scientific | 2004 |
| Ferguson Reed Limited | Households | 2023 |
| Fesco Tiles Limited | Wholesale & retail | 1994 |
| FFE DEV1 Ltd | Extraterritorial | 2025 |
| FFE LH1 Ltd | Extraterritorial | 2025 |
| Fhgc Trading Limited | Hospitality | 2016 |
| Fidus Upholstery Limited | Manufacturing | 2025 |
| Field House Farm (Arable) Limited | Agriculture & fishing | 2025 |
| Field House Farm (Sewerby) Limited | Agriculture & fishing | 2006 |
| Field House Farm (York) Limited | Hospitality | 2015 |
| Fielddale Farriery Limited | Agriculture & fishing | 2020 |
| Fieldwork Light (Research) Limited | Professional & scientific | 2006 |
| Fifth Avenue Fade Ltd | Other services | 2025 |
| Fifth Avenue Haus Ltd | Wholesale & retail | 2026 |
| Filey Brigg Ornithological Group and Bird Observatory Limited | Arts & recreation | 2007 |
| First Catch (Scarborough) Limited | Hospitality | 2012 |
| First Choice Medical Services Limited | Public administration | 2020 |
| Fisheries Research Yorkshire C.I.C. | Professional & scientific | 2025 |
| FL250 Ltd | Financial services | 2024 |
| Flamborough Bird Observatory Trust Limited | Professional & scientific | 2020 |
| Flamborough Marine Limited | Wholesale & retail | 1986 |
| Flowery Dell Limited | Hospitality | 2007 |
| Fmaw Associates Limited | Professional & scientific | 2025 |
| Forefuture Energy Ltd | Extraterritorial | 2025 |
| Forefuture Investments Ltd | Extraterritorial | 2025 |
| Foreman Group Ltd | Financial services | 2021 |
| Forte Maritime Limited | Professional & scientific | 2010 |
| Fortis Envelope Solutions Limited | Construction | 2023 |
| Fountain Farm Children's Nursery Limited | Education | 2008 |
| Fountain Home Care UK Ltd | Health & social care | 2026 |
| Four Oaks Pet Supplies Limited | Wholesale & retail | 2002 |
| Fourzero Media Ltd | Wholesale & retail | 2021 |
| Fowler and SON Maintenance Ltd | Construction | 2026 |
| FOX and Daughters Facilities Management Ltd | Real estate | 2024 |
| Foxholes Garage Limited | Wholesale & retail | 2009 |
| FP Lincoln (250) Limited | Financial services | 2024 |
| Fraisthorpe Beach Car Park Limited | Transport & logistics | 2017 |
| Fraisthorpe Beach Caravans Limited | Hospitality | 2018 |
| Fraisthorpe Renewables Limited | Real estate | 2013 |
| Francis Johnson and Partners Limited | Professional & scientific | 2003 |
| Frasier Costello Limited | Professional & scientific | 2022 |
| Freeman Properties (Yorkshire) Limited | Real estate | 2023 |
| Freetown Developments Limited | Financial services | 2003 |