| Spartan Fork Lift Trucks Limited | Administrative & support | 2009 |
| Spartan Paving & Landscaping Limited | Administrative & support | 2025 |
| Sparx Solutions Limited | Information & communication | 2023 |
| Spatial Insights Ltd | Professional & scientific | 2024 |
| SPC Civil Engineering Ltd | Construction | 2016 |
| SPD E Commerce Ltd | Wholesale & retail | 2025 |
| Spec Vehicle Rentals Limited | Transport & logistics | 2017 |
| Special Pathways Limited | Education | 2022 |
| Special Spices Bar & Grill Ltd | Hospitality | 2025 |
| Specialised Projects Yorkshire Ltd | Construction | 2025 |
| Specialist Autism Services CIC | Health & social care | 2009 |
| Specialist Building Factors Limited | Wholesale & retail | 1989 |
| Specialist Electronics Ltd | Wholesale & retail | 2026 |
| Specialist Emergency Support Ltd | Wholesale & retail | 2024 |
| Specialist People Services EBT Limited | Professional & scientific | 2015 |
| Specialist People Services Group Limited | Financial services | 2022 |
| Specialist Render Supplies (Installations) Limited | Construction | 2007 |
| Specialist Trade Windows Limited | Construction | 2020 |
| Specialist Wholesale Supplies Ltd | Wholesale & retail | 2022 |
| Speciality Processors Limited | Manufacturing | 2001 |
| Specsales (Yorkshire) Ltd | Wholesale & retail | 2015 |
| Spectacular Optics Limited | Health & social care | 2009 |
| Spectracommerce Ltd | Wholesale & retail | 2025 |
| Spectre Properties Limited | Real estate | 2017 |
| Spectrum House Yorkshire Limited | Construction | 2020 |
| Spectrum Security Limited | Administrative & support | 2013 |
| Speed Blinds Ltd | Wholesale & retail | 2022 |
| Speed Fresh Exotics (UK) Limited | Wholesale & retail | 2005 |
| Speedball Indoor Football Limited | Other services | 2010 |
| Speedee Trans Limited | Transport & logistics | 2014 |
| Speedo Bikes Ltd | Wholesale & retail | 2022 |
| Speedx Delivery Limited | Transport & logistics | 2025 |
| Speedy Car Sales West Yorkshire Ltd | Wholesale & retail | 2024 |
| Speedy Cargo Services Ltd | Transport & logistics | 2022 |
| Speedy Export Limited | Wholesale & retail | 2024 |
| Speedy Repairs Mobile UK Ltd | Wholesale & retail | 2019 |
| Speedysleekcustoms Limited | Wholesale & retail | 2024 |
| Speights Logistics Limited | Transport & logistics | 2026 |
| Spektorium Ltd | Information & communication | 2014 |
| Spellman & Walker Group Limited | Manufacturing | 2005 |
| Spellman & Walker Holdings Limited | Professional & scientific | 2019 |
| Spellman & Walker Limited | Manufacturing | 1961 |
| Spenborough Engineering Co.Limited | | 1935 |
| Spencer Technical Solutions Limited | Information & communication | 2016 |
| Spencers of Car Colston Limited | Hospitality | 2003 |
| Sphinx Group Ltd | Construction | 2026 |
| Spice Digital Ltd | Information & communication | 2026 |
| Spice Entertainment Limited | Arts & recreation | 1999 |
| Spice S72 Ltd | Real estate | 2025 |
| Spices of Pakistan Ltd | Hospitality | 2025 |
| Spicy Bite BD6 Ltd | Hospitality | 2025 |
| Spicy Delight Ltd | Manufacturing | 2015 |
| Spicy IT UP Ltd | Hospitality | 2025 |
| Spicy Munch Ltd | Hospitality | 2026 |
| Spicy Options (Wibsey) Limited | Hospitality | 2016 |
| Spicy Vibes Limited | Hospitality | 2024 |
| Spin Arts Ltd. | Arts & recreation | 2021 |
| Spire Assist Limited | Professional & scientific | 2014 |
| Spire Connect Limited | Health & social care | 2024 |
| Spiritual Enlightenment Academy Ltd | Education | 2023 |
| Spiritus Events Ltd | Hospitality | 2025 |
| Spital Hill Garage Limited | Wholesale & retail | 2026 |
| Splash & Dash Mobile Valeting Ltd | Other services | 2023 |
| Splash Services Group Ltd | Administrative & support | 2018 |
| Splash Tech Limited | Wholesale & retail | 2017 |
| Splenify Ltd | Wholesale & retail | 2025 |
| Splice (UK) Limited | Extraterritorial | 2013 |
| Splicegroup Europe Limited | Information & communication | 1991 |
| Splitzbox Ltd | Wholesale & retail | 2023 |
| Spoke Architectural Design Limited | Professional & scientific | 2017 |
| Spooky Limited | Wholesale & retail | 2022 |
| Sport Consult Ltd | Professional & scientific | 2024 |
| Sport on Tour Limited | Arts & recreation | 2023 |
| Sporting Interventions CIC | Arts & recreation | 2025 |
| Sports at Home Limited | Professional & scientific | 2005 |
| Sports Bulk Suppliers Limited | Arts & recreation | 1999 |
| Sports Cafe (BFD) Limited | Hospitality | 2018 |
| Sports Campaign Against Racism Ltd | Education | 2024 |
| Sports Hall Limited | Arts & recreation | 2026 |
| Spot on Cleaners Ltd | Administrative & support | 2025 |
| Spot on Cleaning Services Ltd. | Administrative & support | 2008 |
| Spot Print Ltd | Manufacturing | 2013 |
| Spotless Window Cleaning Yorkshire Limited | Administrative & support | 2023 |
| Spotlight Community Care CIC | Other services | 2026 |
| Spotlynx Cleaning Services Limited | Administrative & support | 2025 |
| SPR Installations Limited | Administrative & support | 2003 |
| Spring Elland Limited | Hospitality | 2019 |
| Spring Gardens Limited | Hospitality | 2019 |
| Spring Lodge Developments Ltd | Construction | 2024 |
| Spring Mount Specialist Care Home Limited | Health & social care | 2010 |
| Spring Place Estate Ltd | Real estate | 2019 |
| Spring Property Acquisitions Ltd | Real estate | 2026 |
| Spring Stores Ltd | Wholesale & retail | 2022 |
| Spring Wood Store Limited | Wholesale & retail | 2023 |
| Springbore Limited | Water & waste | 2017 |
| Springclean (Northern) Limited | Extraterritorial | 1983 |
| Springcliffe Ltd | Health & social care | 2021 |
| Springfield Hall Ltd | Financial services | 2015 |
| Springfield Land Limited | Real estate | 1989 |
| Springhead Bikes Ltd | Wholesale & retail | 2022 |