| Steading Game and Land Management Ltd | Agriculture & fishing | 2025 |
| Steam Punk Retail and Hospitality Ltd | Manufacturing | 2019 |
| Steel Farming Limited | Agriculture & fishing | 2007 |
| Stewart Mccowan Limited | Construction | 2017 |
| Stewart Robertson Projects Limited | Construction | 2006 |
| Stielow Pizza Co Limited | Manufacturing | 2022 |
| Strathardle Trading Community Interest Company | Wholesale & retail | 2009 |
| Strathmore Environmental Services Limited | Professional & scientific | 2013 |
| Strathmore Event Services Ltd | Professional & scientific | 2014 |
| Strathmore Highland Games Ltd | Arts & recreation | 2024 |
| Strathmore Roofing & Coatings Ltd | Construction | 2026 |
| Strone Estates Ltd. | Hospitality | 1998 |
| Susan Rutherford Limited | Wholesale & retail | 2012 |
| Susie Black Fitness Ltd | Health & social care | 2016 |
| Sutherland and Sons Ltd | Real estate | 2025 |
| Swallowtail Lifestyle Limited | Wholesale & retail | 2020 |
| Switchwater Limited | Administrative & support | 2019 |
| Systems Engineering Aberdeen Limited | Professional & scientific | 2015 |
| T R Fine Chemicals Limited | Wholesale & retail | 2013 |
| TAC Geomatics Ltd | Professional & scientific | 2025 |
| Tasteful Ltd | Hospitality | 2025 |
| Taynes Glass Ltd | Wholesale & retail | 2020 |
| Tayside Lettings Ltd | Real estate | 2016 |
| Tayside Pressure Washers (Blairgowrie) LLP | | 2007 |
| TC Labour Ltd | Agriculture & fishing | 2020 |
| TCT - Europe Limited | Professional & scientific | 2019 |
| THE Ardler Tavern Ltd | Hospitality | 2019 |
| THE Auld Brig Ltd | Manufacturing | 2023 |
| THE Building Surveying Company Limited | Professional & scientific | 2015 |
| THE Carbon Removers Limited | Financial services | 2023 |
| THE Cateran Ecomuseum Limited | Arts & recreation | 2018 |
| THE Chippy (Coupar Angus) Ltd | Hospitality | 2016 |
| THE Church Bar Tapas & Grill Ltd | Hospitality | 2024 |
| THE Crown Bar Limited | Hospitality | 2011 |
| THE Dome Blairgowrie Ltd | Hospitality | 2020 |
| THE EYE Store.Com Ltd. | Wholesale & retail | 2002 |
| THE Gael Spirits Company Ltd | Other services | 2018 |
| THE Horny Sheep Company Ltd | Agriculture & fishing | 2024 |
| THE Old Cross Inn Bar & Restaurant Ltd | Hospitality | 2025 |
| THE Old Cross Inn Hotel Ltd | Hospitality | 2025 |
| THE Old Cross Inn Ltd | Administrative & support | 2015 |
| THE Property Girls Lettings Limited | Real estate | 2026 |
| THE Property Girls Partnership Limited | Hospitality | 2022 |
| THE Really Good Life Company Ltd | Hospitality | 2020 |
| THE Swiechs Ltd | Information & communication | 2026 |
| THE Takeaway Blairgowrie Ltd | Hospitality | 2020 |
| THE Travel Bar Limited | Administrative & support | 2003 |
| THE Victoria Blairgowrie Ltd | Hospitality | 2022 |
| THE Wagon Street Food Ltd | Hospitality | 2025 |
| THE Window MAN Dundee Limited | Construction | 2018 |
| Thequicklearncompany.Com Limited | Education | 2025 |
| Thermal Perspective Ltd | Professional & scientific | 2010 |
| Thrifty One CIC | Wholesale & retail | 2020 |
| Tilted Duck Enterprises Limited | Wholesale & retail | 2019 |
| Tilted Duck Ltd | Administrative & support | 2018 |
| TJB Landscaping Services Ltd | Administrative & support | 2015 |
| TNR Fire and Security Ltd. | Administrative & support | 2023 |
| TO the Top Scotland Ltd | Other services | 2026 |
| Tofs (Scotland) Ltd | Arts & recreation | 2000 |
| Torus Tree Ltd | Hospitality | 2024 |
| TP Tools Ltd | Wholesale & retail | 2012 |
| Treemasters Arborist Limited | Administrative & support | 2025 |
| Trend Properties and Interiors Ltd | Real estate | 2013 |
| Trident Foods Ltd | Hospitality | 2026 |
| Truth HZ Ltd | Information & communication | 2019 |
| Tuff Duck Ltd | Administrative & support | 2025 |
| UF02 Ltd | Hospitality | 2024 |
| Urban Rural Ltd | Real estate | 2022 |
| Vanguard Engineering Solutions Limited | Manufacturing | 2014 |
| Veraceylon Ltd | Wholesale & retail | 2025 |
| VIA (Scotland) Limited | Administrative & support | 2015 |
| VIV Medical and Aesthetics Services Limited | Health & social care | 2022 |
| Vizzy Vella Ltd | Financial services | 2025 |
| W.R. Colville & SON | | 1988 |
| WAG Properties Ltd | Real estate | 2023 |
| Wakeboats UK Ltd | Wholesale & retail | 2023 |
| Walker Capital & Investments Ltd | Financial services | 2025 |
| Wester Essendy Holiday Cottages Limited | Real estate | 2020 |
| Westmill Construction Limited | Construction | 2021 |
| Westwood Scotland Ltd. | Construction | 2007 |
| Winton Construction Limited | Construction | 2025 |
| Wired Dundee Ltd | Construction | 2018 |
| Wireless Security Alarms Limited | Construction | 2015 |
| Wood Leisure Holiday Parks Limited | Hospitality | 2018 |
| Woods Properties Blairgowrie Limited | Real estate | 2023 |
| Woodside Cars (Perthshire) Ltd | Wholesale & retail | 2021 |
| Working Place Software Ltd | Information & communication | 2025 |
| Worskel Limited | Real estate | 2021 |
| WPJ Mortgages and Financial Services Ltd | Financial services | 2018 |
| WPJ Properties Ltd | Households | 2026 |
| WRT Builders Ltd | Construction | 2024 |
| Young Energy Services Ltd | Construction | 2021 |
| Your Smiling Mind Ltd | Health & social care | 2021 |
| Zenn Corp Ltd | Construction | 2025 |