| EC Prospect Limited | Financial services | 2009 |
| ECL Logistics Ltd | Transport & logistics | 2022 |
| Ecohomesstore Ltd | Wholesale & retail | 2024 |
| Ecovista PLC | Administrative & support | 2005 |
| Ecozone Limited | Wholesale & retail | 2000 |
| ED Care Services Ltd | Health & social care | 2024 |
| Eden Jewellery UK Limited | Wholesale & retail | 2011 |
| Eden Kane Properties Limited | Real estate | 2017 |
| Edenthorpe Search Ltd | Administrative & support | 2022 |
| Edgehill CS Properties Limited | Real estate | 2025 |
| Edible Adventures Ltd | Hospitality | 2026 |
| Educate African Children and Youth | Education | 2008 |
| Education and Training Systems Ltd | Education | 2023 |
| Education Growth Link Limited | Education | 2024 |
| Eduvi Ltd | Professional & scientific | 2025 |
| Edwards Creative Ltd | Professional & scientific | 2023 |
| Eglinton Consultancy Ltd | Health & social care | 2023 |
| Eiderose Project Management Ltd | Professional & scientific | 2017 |
| EIGHT8 Consultancy Ltd | Professional & scientific | 2024 |
| EIP Solutions Limited | Information & communication | 2012 |
| EJ Consultancy (2017) Limited | Administrative & support | 2017 |
| Ekhinos Limited Liability Partnership | | 2008 |
| Elabel Heritage Properties Ltd | Real estate | 2026 |
| Elam Services Ltd | Real estate | 2024 |
| Elastomer Products Ltd | Wholesale & retail | 2023 |
| Elcl Property Ltd | Real estate | 2026 |
| Eldercare Group Limited | Professional & scientific | 2007 |
| Eldercare Property Partners Limited | Financial services | 2006 |
| Eldercare Solutions Ltd | Financial services | 2005 |
| Elderdell Limited | Other services | 1990 |
| Electrical Contracting Project Management Limited | Professional & scientific | 2003 |
| Electrical Waste Recycling Ltd | Water & waste | 2025 |
| Electrosoft Engineering Limited | Manufacturing | 2013 |
| Elegant DRY Cleaners of Hyde Park Ltd | Administrative & support | 2018 |
| Elements Outdoor Living Limited | Manufacturing | 2024 |
| Elena Taylor Ltd | Information & communication | 2024 |
| ELI Chenkova Business Consultancy & Coaching Ltd | Professional & scientific | 2021 |
| Elif Connect Limited | Professional & scientific | 2024 |
| Eliminatedbt Ltd | Information & communication | 2025 |
| Elite Snaps Limited | Professional & scientific | 2019 |
| Elite Sparks Electrical Ltd | Construction | 2018 |
| ELL Tacos & Churros Limited | Hospitality | 2024 |
| Ellam Aviation Limited | Professional & scientific | 2020 |
| Elodex Products Limited | Wholesale & retail | 2026 |
| ELU Cleaning Services Limited | Administrative & support | 2025 |
| Elucidation Ltd | Professional & scientific | 2025 |
| Emby Electrical Ltd | Construction | 2025 |
| Emcee Contracts Ltd | Information & communication | 2023 |
| Emel (DC) Limited | Other services | 2006 |
| Emily 17 Ltd | Transport & logistics | 2017 |
| Emilymiller Ltd | Professional & scientific | 2021 |
| Eminence Rouge Limited | Arts & recreation | 2025 |
| Eminere Collective Ltd | Wholesale & retail | 2025 |
| Emma COX Limited | Professional & scientific | 2026 |
| Emma Phoenix Flowers Ltd | Wholesale & retail | 2023 |
| Emma PYE Studio Ltd | Professional & scientific | 2023 |
| Empex Tools Ltd | Administrative & support | 2024 |
| Empire Duct Ltd | Construction | 2024 |
| Empire Tools Limited | Wholesale & retail | 2014 |
| Employassist HR Limited | Administrative & support | 2015 |
| Encyclia Aesthetics Ltd | Health & social care | 2024 |
| Encyclia Consulting Ltd | Professional & scientific | 2014 |
| Encyclia Holdings Ltd | Financial services | 2021 |
| Endless Media Ltd | Information & communication | 2012 |
| Endo Packaging Ltd | Wholesale & retail | 2025 |
| Engagewell Ltd | Information & communication | 2024 |
| Enjoy New World Ltd | Wholesale & retail | 2025 |
| Enrich the Agency Limited | Professional & scientific | 2024 |
| Ensocgov Ltd | Professional & scientific | 2023 |
| Ensure Property Services Limited | Construction | 2019 |
| Ensure Property Solutions Limited | Construction | 2021 |
| Enter the Vision Ltd | Information & communication | 2018 |
| Enterprise Fifteen Ltd | Real estate | 2023 |
| Enterprise Fourteen Ltd | Real estate | 2020 |
| Enterprise Growth Group Limited | Financial services | 2022 |
| Entrepreneurs Property Ltd | Real estate | 2022 |
| Entritz Limited | Manufacturing | 2007 |
| Enviro Contract Solutions Ltd | Construction | 2019 |
| Environmental Innovations Limited | Manufacturing | 2007 |
| Enviroshield Pest Management Ltd | Administrative & support | 2021 |
| Envirotec Developments Limited | Real estate | 2022 |
| Envirotech Property Solutions Ltd | Construction | 2022 |
| Epic Sports UK Ltd | Other services | 2017 |
| Epiphanie Coaching Ltd | Other services | 2024 |
| Epitherm Limited | Professional & scientific | 2008 |
| Equibok Limited | Financial services | 2025 |
| Equileben - UK Ltd | Agriculture & fishing | 2016 |
| Equipment HQ Limited | Wholesale & retail | 2016 |
| Equire Property Group Limited | Real estate | 2022 |
| Equity Mortgages Ltd | Financial services | 2018 |
| ER First AID Limited | Health & social care | 2018 |
| ERC Search Limited | Administrative & support | 2013 |
| Erensnkrs Ltd | Wholesale & retail | 2024 |
| Erikson Services Limited | Other services | 2003 |
| Erleigh & Co Cafes Ltd | Hospitality | 2023 |
| Erleigh & Co Ltd | Hospitality | 2019 |
| Ernie & Snap Limited | Administrative & support | 2013 |
| ES Secure Tech Limited | Administrative & support | 2026 |
| ESM Padel Ltd | Arts & recreation | 2026 |
| Essentials at the Maltings Limited | Wholesale & retail | 2008 |