| Angelino's Limited | Hospitality | 2024 |
| Angels Small Paws Dog Rescue | Other services | 2015 |
| ANS Training Solutions Ltd | Construction | 2022 |
| Anthony Andrews Butchers Limited | Wholesale & retail | 2011 |
| Anthony Andrews Catering Ltd | Hospitality | 2021 |
| AOG Properties Limited | Real estate | 2005 |
| APB Residential Sales & Lettings Ltd | Real estate | 2015 |
| Apical Dominance Limited | Professional & scientific | 2015 |
| Apple Industrial Safety Limited | Professional & scientific | 1999 |
| Apply Property Ltd | Households | 2025 |
| Aquaforce (Derbyshire) Limited | Other services | 2011 |
| AR Training Services Limited | Professional & scientific | 2015 |
| ARC Maas Limited | Professional & scientific | 2024 |
| ARC World Wide (UK) Ltd | Professional & scientific | 2003 |
| Arcana Holistic Herbal Ltd | Manufacturing | 2020 |
| Arcanum Ltd | Professional & scientific | 2022 |
| Archie & Amelia's Property Management Services Limited | Real estate | 2020 |
| Architectural Door & Window Solutions Ltd | Manufacturing | 2018 |
| Arctic Windows Derby Limited | Construction | 1999 |
| Argent Antiques Ltd | Wholesale & retail | 2013 |
| Arkwrights Bar Limited | Hospitality | 2014 |
| Arrion Ltd | Professional & scientific | 2026 |
| ARW Transport Limited | Transport & logistics | 2009 |
| AS Built Ltd | Construction | 2015 |
| Asafeplace Ltd | Financial services | 2015 |
| Ashbourne Antiques Centre Limited | Wholesale & retail | 2016 |
| Asherfield Farm Limited | Agriculture & fishing | 2023 |
| Asherfield Livery Limited | Agriculture & fishing | 2026 |
| Ashley Court Associates Limited | Real estate | 2005 |
| Ashton Purser Holdings Ltd | Real estate | 2025 |
| Aspire Civils Ltd | Construction | 2020 |
| Aspire Leisure (Derbyshire) Limited | Hospitality | 2020 |
| Asrc Holdings Ltd | Financial services | 2025 |
| Asset Recovery Agents Ltd | Professional & scientific | 2021 |
| Assured Quality Improvements Limited | Professional & scientific | 2012 |
| Astroturf Recycling Limited | Other services | 2023 |
| AT Rehab Ltd | Health & social care | 2014 |
| Athermont Estates Ltd | Real estate | 2026 |
| Athermont Ltd | Real estate | 2025 |
| ATS Conveyancing Limited | Professional & scientific | 2026 |
| Atunse Studio Limited | Professional & scientific | 2026 |
| Aurea Finance Ltd | Administrative & support | 2025 |
| Authentic Outdoors Limited | Other services | 2021 |
| Auto Additions Nationwide Limited | Manufacturing | 2016 |
| AV & JV Holdings Limited | Financial services | 2023 |
| AV Cars Limited | Transport & logistics | 2024 |
| Avents Events Limited | Arts & recreation | 2024 |
| Aves Aesthetics Ltd | Other services | 2016 |
| Awkward Melon Limited | Professional & scientific | 2016 |
| AWS Electrics Ltd | Manufacturing | 2016 |
| Axiom Security Group Ltd | Administrative & support | 2019 |
| Aytac Express Ltd | Hospitality | 2025 |
| AZP Sales Limited | Wholesale & retail | 2024 |
| B & K Building Services Limited | Construction | 1980 |
| B & K Property Services Limited | Construction | 2001 |
| B & K Structures Limited | Construction | 1974 |
| B Fleet Investments Ltd | Real estate | 2024 |
| B.J.Orme Brickwork and Construction Limited | Construction | 2025 |
| Babbling Vagabonds Limited | Education | 2000 |
| Baby A-Wake Limited | Health & social care | 2012 |
| BAD Bunny Brewery Ltd | Hospitality | 2020 |
| BAF Barber Ltd | Other services | 2025 |
| Bailey Construction (Derby) Holdings Limited | Financial services | 2015 |
| Bailey Property Limited | Real estate | 2017 |
| Bakerville Stays Ltd | Real estate | 2022 |
| Barry Colenso Master Chocolatier Ltd | Manufacturing | 2007 |
| Bath House Farm Ltd | Agriculture & fishing | 2025 |
| Bathie Creative Limited | Professional & scientific | 2024 |
| Bearded Badger Publishing Company Ltd | Information & communication | 2020 |
| Beardsley Decorators Limited | Construction | 1987 |
| Beauty Box Room Ltd | Other services | 2025 |
| Because IT Matters Ltd | Administrative & support | 2015 |
| Beck Foot Consulting Limited | Professional & scientific | 2018 |
| Bedrock Advisory Limited | Professional & scientific | 2024 |
| Belper Arts Festival Limited | Arts & recreation | 2018 |
| Belper Bengal Blues Ltd | Hospitality | 2025 |
| Belper Care Limited | Other services | 1998 |
| Belper Community Enterprise CIC | Arts & recreation | 2016 |
| Belper Community Transport CIC | Transport & logistics | 2025 |
| Belper Developments Limited | Construction | 2006 |
| Belper Food and Wine Ltd | Wholesale & retail | 2022 |
| Belper Freemasons Hall (Property) Ltd | Financial services | 2024 |
| Belper GAS & Heating Ltd | Construction | 2005 |
| Belper Grapes Ltd | Hospitality | 2023 |
| Belper Health Hub Ltd | Health & social care | 2023 |
| Belper Healthcare Ltd | Health & social care | 2023 |
| Belper Kitchen Bathroom and Bedroom Centre Limited | Wholesale & retail | 2025 |
| Belper Leisure Centre Limited | Arts & recreation | 2009 |
| Belper Masonic Hall Ltd | Real estate | 1991 |
| Belper Masonic Regalia Limited | Professional & scientific | 2023 |
| Belper Mobile Phones Ltd | Wholesale & retail | 2025 |
| Belper Nails & Beauty Ltd | Other services | 2020 |
| Belper North Mill Trust | Arts & recreation | 1998 |
| Belper Phones Ltd | Manufacturing | 2025 |
| Belper Podiatry Ltd | Health & social care | 2025 |
| Belper Primary Care Network Limited | Health & social care | 2022 |
| Belper Property Ltd | Real estate | 2026 |
| Belper Rail Engineering Ltd | Professional & scientific | 2014 |
| Belper Trading Limited | Wholesale & retail | 2014 |
| Belper Vapes Ltd | Wholesale & retail | 2025 |