| 1ST Alton Manor Scout Group Limited | Other services | 2009 |
| 3 Fold Strategem Limited | Professional & scientific | 2012 |
| 404 Dynamics Ltd | Manufacturing | 2024 |
| 62/64 Bisson Road Freehold Limited | Households | 2021 |
| @Thedailydalek Ltd | Information & communication | 2026 |
| A Bridge Consultancy Ltd | Other services | 2019 |
| A D Plumbing and Heating Limited | Construction | 2016 |
| A Touch of Beauty Derbyshire Ltd | Other services | 2022 |
| A&J Nails Ltd | Other services | 2025 |
| A-Gradepeople Ltd | Administrative & support | 2025 |
| A. Hingley & SON (Timber) Limited | Agriculture & fishing | 1973 |
| A.L.E.B. Property Ltd | Construction | 2026 |
| A.L.F BUS and Commercial Ltd | Wholesale & retail | 2024 |
| A.M.C Transport Solutions Holdings Limited | Financial services | 2023 |
| A.M.C Transport Solutions Ltd | Transport & logistics | 2018 |
| A.S Trenchless Services Ltd | Construction | 2026 |
| A1 Aerospace Ltd | Information & communication | 2012 |
| AA Bespoke Ltd | Manufacturing | 2020 |
| Aandaz Ltd | Hospitality | 2026 |
| AB Connected Ltd | Transport & logistics | 2024 |
| Abase Developments Ltd | Construction | 2022 |
| Abro Engineers Limited | Professional & scientific | 2003 |
| Access Locks (Derby) Limited | Administrative & support | 2007 |
| Accounting Solutions (Great Britain) Limited | Professional & scientific | 2005 |
| ACJ Family Investment Company Limited | Real estate | 2025 |
| ACK Management Limited | Professional & scientific | 2004 |
| Acme Site Services Ltd | Professional & scientific | 2026 |
| Acorn Safety Consultants Ltd | Administrative & support | 2008 |
| Acres Farm (Belper) Ltd | Real estate | 2025 |
| Action for Care Contracts Limited | Professional & scientific | 2020 |
| Adaptive Web Limited | Information & communication | 2003 |
| Adcock Refrigeration and Air Conditioning Limited | Manufacturing | 1977 |
| Addc Limited | Hospitality | 2009 |
| Adhd United Group Limited | Information & communication | 2025 |
| Adimpletio Ltd. | Professional & scientific | 2017 |
| ADJ Global Green Energy Limited | Wholesale & retail | 2024 |
| Adrian Heapy Photography Ltd | Professional & scientific | 2010 |
| Adrian S Wood Ltd | Other services | 2017 |
| ADS Power Networks Ltd | Construction | 2026 |
| Aevitas Black Ltd | Real estate | 2019 |
| Aevitas Green Ltd | Real estate | 2019 |
| Aevitas Property Ltd | Real estate | 2019 |
| Agcon Limited | Agriculture & fishing | 2004 |
| Ageless by Design Limited | Health & social care | 2026 |
| Agility Secrets Ltd | Arts & recreation | 2022 |
| Aglio Limited | Professional & scientific | 2023 |
| AIB Psychology Ltd | Professional & scientific | 2022 |
| AJL Property Services Limited | Real estate | 2021 |
| AKB Haulage Limited | Transport & logistics | 2015 |
| Akers Avia Ltd | Manufacturing | 2024 |
| AL Diablo Ltd | Professional & scientific | 2024 |
| Alan Benfield Holdings Limited | Professional & scientific | 2004 |
| Alan Benfield Limited | Construction | 1991 |
| Alderville Enterprises Limited | Agriculture & fishing | 1987 |
| Alet Marketing Limited | Professional & scientific | 2017 |
| Alex Webb Property Ltd | Administrative & support | 2025 |
| Alexander Architecture Ltd | Professional & scientific | 2023 |
| Alexridge Limited | Real estate | 1995 |
| ALF Properties Ltd | Real estate | 2017 |
| ALI Property Developments Europe (2007) Limited | Real estate | 2007 |
| Alison & Freddie Limited | Wholesale & retail | 2024 |
| ALL in Good Taste Limited | Health & social care | 2012 |
| ALL Things Local Limited | Professional & scientific | 2009 |
| ALL Video Productions Ltd | Information & communication | 2015 |
| Allajbej Holding Investments Ltd | Real estate | 2024 |
| Allestree Aesthetics Limited | Health & social care | 2017 |
| Alpac Alloys Limited | Manufacturing | 1989 |
| Alpat Systems Ltd | Information & communication | 2006 |
| Alphabet Soup Limited | Information & communication | 2011 |
| Alvaston Scaffolding Ltd | Construction | 2024 |
| Always Lucky Limited | Other services | 2024 |
| AM Fabrication Service Ltd | Manufacturing | 2018 |
| Amaargi Software Ltd | Information & communication | 2022 |
| Amanda FAY Aesthetics Limited | Other services | 2026 |
| Amber Energy Surveys Limited | Real estate | 2007 |
| Amber Gardens Management Company Limited | Real estate | 2021 |
| Amber Green Ltd | Real estate | 2021 |
| Amber Hire Co. Ltd | Extraterritorial | 1997 |
| Amber Lifting Solutions Ltd | Administrative & support | 2024 |
| Amber MIX Limited | Extraterritorial | 2024 |
| Amber Valley Homes Ltd | Construction | 1998 |
| Amber Valley Investments Limited | Professional & scientific | 2014 |
| Amber Valley Logistics Limited | Transport & logistics | 2017 |
| Amber Valley Phones Ltd | Manufacturing | 2025 |
| Amber Valley Plant Hire Ltd | Construction | 2022 |
| Amber Valley Plant Sales Ltd | Manufacturing | 2024 |
| Amber Valley Surveyors Limited | Professional & scientific | 2020 |
| Amber Valley Transport Limited | Transport & logistics | 2012 |
| Amber Valley Vapes Ltd | Wholesale & retail | 2025 |
| Amberbuild Ltd | Construction | 2025 |
| Ambergate Property Services Limited | Construction | 2004 |
| Ambertara Limited | Hospitality | 2018 |
| Amffra Consulting Ltd | Professional & scientific | 2015 |
| AMO Raju & Associates Ltd | Administrative & support | 2013 |
| Ampego Ltd | Other services | 2020 |
| Ample Imports Limited | Extraterritorial | 2000 |
| Andrew Churchman Limited | Construction | 2015 |
| Andrew Electrical Co. Ltd | Construction | 2015 |
| Andrew Sellors Electrical Ltd | Construction | 2024 |
| Andrewbcooper Ltd | Wholesale & retail | 2023 |