| Cutting Edge Improvements Limited | Construction | 2003 |
| D & G Consultancy | | 2005 |
| D & S Lindsay (Ames Taping Contractors) Limited | Construction | 2003 |
| D-I-Y Ayrshire Limited | Wholesale & retail | 2006 |
| D. Bryson (Newmilns) Limited | Agriculture & fishing | 1945 |
| Dalry Inn Limited | Hospitality | 2016 |
| David Cook Developments Limited | Professional & scientific | 1996 |
| David Ogilvie Engineering Limited | Manufacturing | 1996 |
| David Ogilvie International Limited | Manufacturing | 2004 |
| Delanvale Limited | Hospitality | 1990 |
| Derbrook Limited | Administrative & support | 2005 |
| DI Vinci Limited | Hospitality | 1994 |
| Divine & Gibson Limited | Wholesale & retail | 1949 |
| DLW Fitness Ltd | Arts & recreation | 1997 |
| Dormy Developments Limited | Real estate | 2007 |
| Douncroft Limited | Financial services | 1990 |
| Duffield Morgan Limited | Real estate | 1995 |
| Dumfries House Trust Trading Limited | Hospitality | 2007 |
| Duncan Plant Hire Limited | Administrative & support | 1999 |
| Duncanziemere Farm | | 1985 |
| Dunnymuck Farm NO.2 | | 1997 |
| Duskview Services Limited | Wholesale & retail | 2005 |
| Dussie Ltd | Real estate | 2001 |
| Dustacco Engineering Limited | Manufacturing | 1966 |
| E I Property Management Limited | Real estate | 2003 |
| East & West Mills Limited | Professional & scientific | 2004 |
| Easton Capital Limited | Financial services | 2020 |
| Edinburgh Pharmaceutical Industries Limited | Professional & scientific | 1904 |
| EK Kebab Ltd | Hospitality | 2022 |
| Electro Installations (Scotland) Limited | Construction | 1973 |
| Electronics Link Europe Ltd. | Professional & scientific | 2000 |
| Elmbronze Limited | Manufacturing | 1994 |
| Elmhurst Transport (Scotland) Ltd. | Transport & logistics | 1997 |
| Envirocote Limited | Wholesale & retail | 1999 |
| Environmental and GAS Monitoring Limited | Professional & scientific | 2000 |
| Euro Driveshafts & Hydraulics Limited | Manufacturing | 1984 |
| Evergreen Sustainable Energy Ltd | Professional & scientific | 2026 |
| Exordia Software Limited | Information & communication | 1998 |
| Fabtech (Scotland) Limited | Manufacturing | 1995 |
| Fardens Farms | | 1991 |
| Fencebay Limited | Agriculture & fishing | 2003 |
| Figgitt Services Limited | Manufacturing | 1998 |
| First Rule Investment Consultancy Limited | Administrative & support | 2005 |
| Firthside Limited | Energy | 2021 |
| Forsyth Project Management Ltd | Construction | 2018 |
| Freeman Engineering Ltd. | Professional & scientific | 2007 |
| Friends of Culzean | Arts & recreation | 2007 |
| Futurama Properties Limited | Real estate | 2007 |
| G Marshall Farming | | 2002 |
| G.I. Young & Company Limited | Agriculture & fishing | 1926 |
| Gemmell's Garden Centre Ltd | Wholesale & retail | 2005 |
| GH Resourcing Limited | Administrative & support | 1993 |
| Girvan Mains Partnership | | 1985 |
| Glasgow School of Shiatsu Limited | Education | 1993 |
| Glen Services (W.K.) Limited | Construction | 2003 |
| Glengairn Properties Limited | Real estate | 2004 |
| Glenton Partnership | | 1986 |
| Gorderton Limited | Real estate | 2006 |
| GPE Team Sports Ltd | Wholesale & retail | 2016 |
| Graceland Nursing Home Limited | Health & social care | 1999 |
| Grahamston Limited | Construction | 1981 |
| GRD Trowel Trades Ltd | Construction | 2014 |
| Greenhollow Limited | Professional & scientific | 2000 |
| Grid Consulting Limited | Information & communication | 2004 |
| GSC Timber Ltd. | Construction | 2004 |
| H. Crawford Limited | Professional & scientific | 1992 |
| H.K.B. Systems Limited | Construction | 1985 |
| Haddow Aird & Crerar Limited | Manufacturing | 1991 |
| Hadley Wood Health Care Centre Limited | Health & social care | 1992 |
| Halcion Limited | Professional & scientific | 2004 |
| Halliday Leisure Limited | Administrative & support | 2002 |
| Hansel Alliance | Health & social care | 1998 |
| Hansel Foundation | Health & social care | 1963 |
| Harkiss Developments Limited | Real estate | 1998 |
| Harkiss Homes (Scotland) Limited | Real estate | 1998 |
| Harperfield Limited | Extraterritorial | 1999 |
| HCP1 Limited | Mining & quarrying | 2020 |
| Hendrie Brothers (Millands) Limited | Agriculture & fishing | 1944 |
| Higgins Sifton Fabrication Limited | Extraterritorial | 2004 |
| High Park Hill | | 1991 |
| Hillhead Farming Company | | 1991 |
| Hillhouse Estates Limited | Mining & quarrying | 1945 |
| Hillhouse Events Limited | Hospitality | 2006 |
| Hillhouse Quarry Group Limited | Mining & quarrying | 1991 |
| HMC Holdings Limited | Real estate | 1904 |
| HMC Improvements Ltd. | Extraterritorial | 1969 |
| Hobencourt Limited | Hospitality | 1990 |
| Howford Hydraulics Limited | Administrative & support | 2001 |
| Howford Ltd | Real estate | 2001 |
| Howgate Properties and Letting Limited | Real estate | 2005 |
| Howie's Garage Limited | Wholesale & retail | 1993 |
| Hugh Ferguson & Company Limited | Professional & scientific | 2003 |
| Hydro Services (Seamill) | Other services | 2005 |
| Hyspec Engineering Holdings Limited | Financial services | 2010 |
| Hyspec Engineering Limited | Manufacturing | 2010 |
| I C Systems (Scotland) Limited | Professional & scientific | 1992 |
| Iain Tweedie Ltd. | Agriculture & fishing | 2004 |
| IAN Gillan Plant Hire and Sales Limited | Administrative & support | 2004 |
| Impact Training Ltd. | Professional & scientific | 2006 |
| Inamo Limited | Health & social care | 1981 |