| 1ST Golden Star Limited | Hospitality | 2010 |
| 247 Cargo Limited | Professional & scientific | 2005 |
| A & J Stitt | | 1995 |
| A J H Builders Ltd | Construction | 2003 |
| A&L Bryces Limited | Wholesale & retail | 2018 |
| A. Stevely Electrical Contractors Limited | Construction | 1995 |
| A.L. Paint and Supplies Limited | Wholesale & retail | 1997 |
| Acre Lettings Limited | Real estate | 1994 |
| Active Risk Management Ltd. | Professional & scientific | 2004 |
| Aero Turbine Maintenance Limited | Manufacturing | 2003 |
| Agri-Input Direct Limited | Wholesale & retail | 2000 |
| AJ Simpson Engineering Limited | Professional & scientific | 2015 |
| Alba Engineering & Recruitment Services Ltd. | Administrative & support | 2007 |
| Albion Environmental Limited | Professional & scientific | 2003 |
| Alex Bicket Limited | Manufacturing | 1982 |
| Alexander George & Co (Investments) Ltd. | Households | 1983 |
| Alexanders’ SAW Mills Holdings Limited | Financial services | 2024 |
| Allanton Renewables Limited | Energy | 2024 |
| Allegra Properties Limited | Real estate | 2007 |
| Allsorts Transport & Trading Limited | Transport & logistics | 1998 |
| Alps (Scotland) Limited | Construction | 2002 |
| Amer Sports UK Services Limited | Manufacturing | 1961 |
| Annandale Design (U.K.) Limited | Manufacturing | 1992 |
| Anne Tweedie Limited | Other services | 2003 |
| Anteolas Limited | Professional & scientific | 2002 |
| Apex Auto Electrics Limited | Wholesale & retail | 2003 |
| Arcade Building Services Limited | Construction | 1972 |
| Ardrossan Finance Limited | Real estate | 1985 |
| Artane Solutions Limited | Professional & scientific | 2001 |
| Auchengate Sawmills (Irvine) Limited | Administrative & support | 2004 |
| Auchenskeith Farms | | 1992 |
| AYR Baptist Homes | Health & social care | 1988 |
| Ayrconsult Limited | Real estate | 2005 |
| Ayrshire Building Construction Ltd. | Construction | 2004 |
| Ayrshire Financial Services Ltd. | Financial services | 2000 |
| Ayrshire Flooring Company Limited | | 1966 |
| Ayrshire Veterinary Services Ltd. | Professional & scientific | 1999 |
| B F Roofing Limited | Construction | 2009 |
| Ballochmyle Farming Co Ltd. | Agriculture & fishing | 2009 |
| Balsillie Farm Limited | Agriculture & fishing | 1997 |
| Barns Dental Practice Ltd | Health & social care | 2006 |
| Barr Findlay Partnership | | 1977 |
| Barr-Banniser Partnership | | 1988 |
| Bellimore Farmers | | 1995 |
| Benan Limited | Administrative & support | 2007 |
| Bennett's (Scotland) Limited | Wholesale & retail | 1993 |
| Billy Bowie Special Projects Limited | Water & waste | 1999 |
| Billy Bowie Tanker Services Limited | Extraterritorial | 1997 |
| Black Property Co. Limited | Real estate | 2006 |
| Bluebells Properties Limited | Real estate | 2023 |
| Borders Straw Limited | Agriculture & fishing | 2002 |
| Borwick/Hugh Gilbert | | 1988 |
| Borwick/Kirkpatrick | | 1987 |
| Borwick/Thomas Smith | | 1987 |
| Brian Hart Ltd. | Construction | 2002 |
| Brockloch 1999 | | 2000 |
| Brown's Pressure Washers Limited | Administrative & support | 2005 |
| Buckside Limited | Energy | 2023 |
| Bungydome (UK) Ltd. | Arts & recreation | 2007 |
| Burnawn Accountancy Services Ltd | Professional & scientific | 2006 |
| Burnbank Pet Hotel Limited | Wholesale & retail | 2010 |
| Burns Developments Limited | Real estate | 1998 |
| Burns Pharmacy Limited | Wholesale & retail | 2003 |
| Burns Real ALE Ltd. | Real estate | 1995 |
| Business Support & Advice Europe Ltd | Professional & scientific | 2007 |
| C & C Custom Stainless Exhausts Limited | Wholesale & retail | 2003 |
| C & N (Publishing) Limited | Extraterritorial | 1997 |
| C & N Records Limited | Administrative & support | 1995 |
| Cairn Mushrooms | | 2001 |
| Cairngrove Limited | Other services | 2002 |
| Calebridge Limited | Financial services | 1999 |
| Calleili Limited | Professional & scientific | 2007 |
| Cards ET Cetera Limited | Wholesale & retail | 2000 |
| Carpet Clearance Limited | Wholesale & retail | 2003 |
| Carthon Lets Limited | Real estate | 1997 |
| Casa Construction Limited | Construction | 1999 |
| Cbgb Holdings Limited | Financial services | 2007 |
| CCL Logistics Ltd | Transport & logistics | 1999 |
| CDS (Information Technology) Ltd | Information & communication | 1999 |
| Celtic Mail Order Company | | 1994 |
| Central Avenue Limited | Hospitality | 2012 |
| Changue Farm & Company | | 1988 |
| Chapelton Farms | | 1986 |
| Charles Buyers & Co. Ltd. | Manufacturing | 1978 |
| Charles Chester(Jewellery) Ltd. | Wholesale & retail | 2001 |
| Chrisart Inc. | | 1996 |
| City Tyres & Exhausts Limited | Real estate | 1998 |
| Classique Clothing Company | | 1994 |
| Classique Decor Limited | Construction | 2007 |
| Cleeves Cabins Ltd | Hospitality | 2021 |
| Clyde Coast Contracts Limited | Construction | 1979 |
| Clyde Logistics Solutions Ltd | Transport & logistics | 2026 |
| Clyde Marina Limited | Arts & recreation | 1995 |
| Coffeepress Limited | Hospitality | 2006 |
| Coila Consulting Limited | Professional & scientific | 2003 |
| Collennan Ltd. | Professional & scientific | 2003 |
| Country Courtyard Cottages Limited | Real estate | 1999 |
| County Properties Scotland Limited | Real estate | 2004 |
| Crossroads (South Ayrshire) Care Attendant Scheme | Health & social care | 1995 |
| Crown Hotel (the) | | 1988 |