| R&P Alterations Limited | Other services | 2025 |
| R.D. Designs Limited | Professional & scientific | 1988 |
| R.E.N Services (Scotland) Limited | Administrative & support | 2020 |
| R8 Groundworks and Landscaping Ltd | Administrative & support | 2023 |
| Racecourse Rooms Ltd | Real estate | 2025 |
| Rachel Limitless Living Ltd | Other services | 2022 |
| Rackit Group Ltd | Other services | 2020 |
| Radant Limited | Construction | 2024 |
| Radbury Double Glazing Ltd | Construction | 2010 |
| Raffinato Venetian Plastering Ltd | Construction | 2021 |
| Rahans Place Ltd | Wholesale & retail | 2024 |
| Rainbow Fizz Ltd | Wholesale & retail | 2026 |
| Ramage Estates Limited | Real estate | 2014 |
| Ramage Holdings Ltd | Real estate | 2021 |
| Rankin Holdings Scotland Limited | Financial services | 2018 |
| Rarer Ltd | Hospitality | 2025 |
| Rasa Properties (AYR) Ltd | Real estate | 2022 |
| Rasm Property Ltd | Real estate | 2025 |
| Rawcayr Ltd | Wholesale & retail | 2018 |
| Raymond Buchanan Decorators Ltd | Construction | 2021 |
| Rayton Trade LP | | 2014 |
| Raza & Co (Scot) Ltd | Hospitality | 2018 |
| RC Coachworks Ltd | Wholesale & retail | 2021 |
| RCS Rentals Limited | Real estate | 2023 |
| RDA Roofing Services Limited | Construction | 2025 |
| RDP (AYR) Limited | Financial services | 2007 |
| Rdwm Limited | Wholesale & retail | 2025 |
| Readyjunk Limited | Water & waste | 2017 |
| Rebelle Limited | Other services | 2015 |
| Recence Ltd | Information & communication | 2017 |
| Red Circle Safety Limited | Professional & scientific | 2018 |
| Redemption Tattoo Club Ltd | Other services | 2023 |
| Redford Care Home Ltd. | Professional & scientific | 2010 |
| Redgate Timber Frame Limited | Construction | 2020 |
| Reference Interiors Limited | Construction | 2020 |
| Reflection Films Ltd | Information & communication | 2022 |
| Regency FM Ltd | Administrative & support | 2024 |
| Regency Removals & Storage Limited | Transport & logistics | 2021 |
| Reids Trophies (Kilmarnock) Ltd | Wholesale & retail | 2013 |
| Reids Trophies Ltd | Wholesale & retail | 2013 |
| Reilly Roofing & Builders Ltd | Construction | 2022 |
| Release the Bats Ltd | Households | 2018 |
| Reliable Spine Ltd | Wholesale & retail | 2024 |
| Rellec Ltd | Manufacturing | 2025 |
| Remarkable Law Consultancy Limited | Professional & scientific | 2019 |
| Remarket Homes Limited | Real estate | 2025 |
| Remedy Roofing Limited | Construction | 2023 |
| Rennie Property Limited | Real estate | 2023 |
| Rent-A-Vibe Ltd | Administrative & support | 2026 |
| Repair GAS Ltd | Construction | 2019 |
| Repartner Limited | Professional & scientific | 2016 |
| Repayr My Car Ltd | Wholesale & retail | 2022 |
| Republic of Munchery Limited | Manufacturing | 2015 |
| Request & Quest Limited | Wholesale & retail | 2022 |
| Research Scotland Ltd | Professional & scientific | 2015 |
| Reserve Assets LP | | 2014 |
| Resolve 360 Planning Ltd | Professional & scientific | 2025 |
| Restfood Limited | Hospitality | 2024 |
| Revive Health and Beauty Group Limited | Wholesale & retail | 2024 |
| RF Savvy Technologies Limited | Information & communication | 2026 |
| RFG Financial LP | | 2014 |
| RG Wilson Limited | Agriculture & fishing | 2025 |
| RGW Plumbing & Building Solutions Limited | Construction | 2021 |
| RH 1111 Ltd | Construction | 2016 |
| RH01 Ltd | Real estate | 2017 |
| RH03 Limited | Real estate | 2018 |
| RH05 Limited | Real estate | 2018 |
| RH06 Ltd | Real estate | 2018 |
| RH07 Ltd | Real estate | 2018 |
| RH08 Ltd | Real estate | 2017 |
| RH09 Ltd | Real estate | 2017 |
| RHA Construction (AYR) Ltd | Construction | 2019 |
| Rhino Storage Solutions Ltd | Real estate | 2021 |
| Rhobert Systems LP | | 2014 |
| Rice Contracts Limited | Professional & scientific | 2014 |
| Richard Wade Joinery Ltd | Construction | 2021 |
| Richmond Deagle Limited | Real estate | 2016 |
| RIGHT2USE Limited | Information & communication | 2002 |
| Rileys Mechanical Services Ltd | Manufacturing | 2018 |
| Ringside VIP Ltd | Other services | 2025 |
| Rithu Ltd | Wholesale & retail | 2024 |
| Riva Residential Limited | Real estate | 2025 |
| River Doon Trout Company Limited | Agriculture & fishing | 1980 |
| Riverside Studios SCI Ltd | Professional & scientific | 2020 |
| Rjborland Limited | Wholesale & retail | 2025 |
| RJM Engineering Services Ltd | Construction | 2026 |
| RJM Tuning Ltd | Wholesale & retail | 2025 |
| RJN Automotive Limited | Wholesale & retail | 2015 |
| RJT Flooring Ltd | Other services | 2024 |
| RK Project Solutions Ltd | Construction | 2025 |
| RLC Joinery Ltd | Manufacturing | 2023 |
| Rlcm Trading Ltd | Health & social care | 2021 |
| RM Bodyworks Ltd | Wholesale & retail | 2021 |
| RM Cleanview Services Ltd | Administrative & support | 2026 |
| RMC Double Glazing (AYR) Ltd | Construction | 2013 |
| RMC Double Glazing Trade (AYR) Ltd | Construction | 2023 |
| RMK Cable Jointing Ltd | Energy | 2025 |
| RMM Accounting & Consultancy Limited | Professional & scientific | 2026 |
| RMM Operations Ltd | Hospitality | 2024 |
| RMS Gardening & Ground Maintenance Ltd | Administrative & support | 2024 |