| Elivito Ltd | Health & social care | 2024 |
| Elizabeth Divine Limited | Information & communication | 2012 |
| Elizabeth Warnes Limited | Professional & scientific | 2003 |
| Ellams Duplicator Company,Limited | Extraterritorial | 1910 |
| Ellesborough Golf Club Limited | Arts & recreation | 2018 |
| Ellesborough Golf Company,Limited(The) | Real estate | 1930 |
| Ellie Engineering Limited | Transport & logistics | 2017 |
| Ellis & SON Property Ltd | Real estate | 2023 |
| Ellis Enterprises (UK) Limited | Construction | 2005 |
| Ellis Laurel Finance Limited | Financial services | 2022 |
| Ellisloen 1968 Limited | Extraterritorial | 2014 |
| Ello Sunshine 1964 Limited | Extraterritorial | 2014 |
| ELM Farm Tandoori Limited | Hospitality | 2010 |
| ELM Leys (Management Company) Ltd | Households | 2019 |
| ELM Square Advisers Limited | Professional & scientific | 2015 |
| Elmer Consultancy Ltd | Professional & scientific | 2019 |
| Elmhurst Garage Ltd | Wholesale & retail | 2017 |
| Elmstree LLP | | 2015 |
| Elmtree Housing Ltd | Real estate | 2022 |
| Elnet Consulting Ltd | Information & communication | 2013 |
| Elohiym Ltd | Other services | 2016 |
| ELS One Limited | Construction | 2026 |
| Elsmore Clinic Limited | Health & social care | 2019 |
| Elson Project Management Limited | Professional & scientific | 2012 |
| Elvum Investments Ltd | Real estate | 2022 |
| Elysian Property Ltd | Real estate | 2021 |
| Elysium Consultancy Services Ltd | Professional & scientific | 2016 |
| Emanzini Assessments & Consultancy Ltd | Health & social care | 2022 |
| Emasam Limited | Other services | 2026 |
| Embroiderers Guild (the) | Education | 1934 |
| EME Nails and Cosmetics Ltd | Other services | 2025 |
| Emerald Brows Limited | Other services | 2013 |
| Emhr Group Limited | Professional & scientific | 2024 |
| Emilex Ltd | Information & communication | 2023 |
| Emily Brigden Limited | Professional & scientific | 2010 |
| Emily Jonzen Limited | Professional & scientific | 2015 |
| Eminent Design Ltd | Wholesale & retail | 2018 |
| Emirates Transport Ltd | Transport & logistics | 2022 |
| Emiyo Ltd | Wholesale & retail | 2026 |
| Emma Flowers Ltd | Professional & scientific | 2022 |
| Emma Kendall Ltd | Professional & scientific | 2020 |
| Emmanuel Wisdom Health Care Limited | Health & social care | 2015 |
| Emma’S Mobile Bars Ltd | Hospitality | 2024 |
| Emmerich Ltd | Wholesale & retail | 2024 |
| Empathy Care Solutions Ltd | Health & social care | 2022 |
| Empathéa Care & Support Ltd | Health & social care | 2025 |
| Empire Construction Services Limited | Construction | 2004 |
| Empire Landscapes Ltd | Administrative & support | 2018 |
| Empire Sports Group UK Limited | Professional & scientific | 2024 |
| Empower Education Tuition Ltd | Education | 2026 |
| Empower Occupational Therapy Ltd | Health & social care | 2024 |
| Empower-U Services Limited | Health & social care | 2020 |
| Empowered Living Foundation Ltd | Other services | 2004 |
| Empress Solutions Ltd | Real estate | 2018 |
| EMZ Consultants Ltd | Information & communication | 2007 |
| ENC Techworks Limited | Wholesale & retail | 2025 |
| Encav Tech Limited | Wholesale & retail | 2025 |
| Enceladus Communications Ltd | Professional & scientific | 2014 |
| Encommon Limited | Professional & scientific | 2021 |
| Encompass Building Solutions Ltd | Construction | 2019 |
| Encourage International Limited | Other services | 2016 |
| END Grain Group Ltd | Financial services | 2026 |
| END Slate Ltd | Other services | 2020 |
| Endamar Academy Ltd | Education | 2023 |
| Endean Builders Ltd | Construction | 2015 |
| Endless Data and B.I. Services Ltd | Information & communication | 2014 |
| Endocrine Consult Limited | Other services | 2022 |
| Enduran Limited | Professional & scientific | 2019 |
| Enercon Industries Limited | Manufacturing | 1992 |
| Energea Radionics Ltd | Professional & scientific | 2004 |
| Energy at Long Crendon Limited | Professional & scientific | 2013 |
| Energy of Life Limited | Professional & scientific | 2009 |
| Engine Race Events Ltd | Arts & recreation | 2026 |
| Enginero Ltd | Professional & scientific | 2022 |
| Englefield Scaffolding and Roofing Limited | Construction | 2026 |
| English Bridge Union Limited(The) | Other services | 1940 |
| Enhance the UK | Other services | 2009 |
| Enhancion Limited | Information & communication | 2022 |
| Enho Project Management Consultants Ltd | Administrative & support | 2017 |
| Enrich Education and Training Ltd | Education | 2022 |
| Entecott Nicholls Limited | Professional & scientific | 2019 |
| Enterprise Cabs Limited | Transport & logistics | 2023 |
| Enterprise Hire Ltd | Administrative & support | 2025 |
| Enterprise Skip and Grab Hire Limited | Water & waste | 2018 |
| Entertainment Inflatable Rental Ltd | Administrative & support | 2026 |
| Entertainments R US Ltd | Administrative & support | 2016 |
| Entourage Holdings Limited | Construction | 2024 |
| Entrust Care Ltd | Administrative & support | 2019 |
| Entrust Consultancy Limited | Professional & scientific | 2023 |
| Entrust Global Facilities Services Ltd | Health & social care | 2019 |
| Entrust Global Trading Ltd | Wholesale & retail | 2017 |
| Entrust Group Limited | Financial services | 2023 |
| Envestia Limited | Administrative & support | 2007 |
| Enviro Sewage Treatment Services Limited | Water & waste | 2013 |
| Environment and Development Services Limited | Professional & scientific | 2014 |
| Environmental Cooling Systems Limited | Construction | 1993 |
| Environmental GAS Care Ltd | Education | 2021 |
| Environmental Treatment Systems Limited | Manufacturing | 1999 |
| Environmentum Ltd | Professional & scientific | 2022 |
| Envision Carpentry Limited | Construction | 2022 |