| Barkology Limited | Other services | 2024 |
| Barlow Grove (Wendover) Management Company Limited | Households | 2014 |
| Barmakian Ltd | Wholesale & retail | 2015 |
| Barnaby AI Limited | Wholesale & retail | 2021 |
| Barneypal Limited | Real estate | 2010 |
| Barnowls Nursery Limited | Health & social care | 2009 |
| Baronage Limited | Professional & scientific | 2005 |
| Barrala Limited | Extraterritorial | 2019 |
| Barrbell Productions Limited | Administrative & support | 2024 |
| Barrels Accounting Ltd | Professional & scientific | 2011 |
| Barry Marcello Vehicle Repairs Limited | Wholesale & retail | 2016 |
| Barry Westwood Productions | | 1992 |
| Bart 401 Limited | Agriculture & fishing | 2012 |
| Bartekkalan Ltd | Transport & logistics | 2016 |
| BAS Accountancy & Tax Services Ltd | Extraterritorial | 2016 |
| BAS Accounting Services Ltd | Extraterritorial | 2020 |
| BAS Associates Limited | Professional & scientific | 2004 |
| BAS Consultancy Services Ltd | Professional & scientific | 2016 |
| BAS Property & Investments Ltd | Professional & scientific | 2004 |
| BAS Tax & Accountancy Services Ltd | Extraterritorial | 2016 |
| Base Edit Ltd | Professional & scientific | 2022 |
| Base Property Portfolio Limited | Real estate | 2020 |
| Base Tech Pro Audio Ltd | Information & communication | 2022 |
| Baseaid Consultancy Limited | Administrative & support | 2023 |
| Basement 13 Tattoos Ltd | Other services | 2017 |
| Bathroom Rejuvenation Ltd | Other services | 2023 |
| Batpie Music Limited | Information & communication | 2022 |
| Batt Electrical Ltd | Construction | 2023 |
| Baurbhag Limited | Hospitality | 2026 |
| Baust Ltd | Information & communication | 2024 |
| Bawer Grill Ltd | Hospitality | 2020 |
| Baxter & Baxter Limited | Construction | 2003 |
| Bay 19 Golf Ltd | Arts & recreation | 2022 |
| Bayit Properties Limited | Households | 2022 |
| Bayside Medical Limited | Health & social care | 2010 |
| Baytec Associates Aylesbury Ltd | Construction | 2011 |
| BBD Timberworks Limited | Manufacturing | 2007 |
| BBE Homes & Property Ltd | Health & social care | 2026 |
| BBE Property Services Ltd | Construction | 2023 |
| BBG Associates Limited | Education | 1999 |
| Bcbj Limited | Wholesale & retail | 2020 |
| BCF Heating & Plumbing Ltd | Construction | 2006 |
| BCH Limited | Hospitality | 2025 |
| BCS Hospitality Ltd | Hospitality | 2025 |
| BDY365 Limited | Arts & recreation | 2023 |
| BE Good Limited | Administrative & support | 2004 |
| BE IT UN Limited | Information & communication | 2017 |
| Beachview (Porthminster) Management Company Limited | Real estate | 2002 |
| Beacon Communications and Print Limited | | 1987 |
| Beacon Evolution Limited | Real estate | 2017 |
| Beacon Gray Consulting Limited | Information & communication | 2025 |
| Beadie Limited | Information & communication | 2014 |
| Bean Marketing Ltd | Professional & scientific | 2026 |
| Bearbrook Construction Limited | Construction | 2005 |
| Bearing Traders Limited | Wholesale & retail | 1986 |
| Bearings Scaffold Services Limited | Construction | 2006 |
| Beast Production Location Services Ltd | Professional & scientific | 2025 |
| Beat for Health Ltd | Health & social care | 2022 |
| Beaubury Holdings Limited | Financial services | 1999 |
| Beaubury Precision Moulds Limited | Manufacturing | 1964 |
| Beaumont and Cowling (Wendover) Limited | Professional & scientific | 1993 |
| Beautchoc Limited | Manufacturing | 2021 |
| Beautiful Bathrooms (Hayes) Limited | Construction | 1994 |
| Beauty Blush Wendover Ltd | Other services | 2025 |
| Beauty by Bryony Ltd | Other services | 2022 |
| Beauzee Valeting & Detailing Limited | Administrative & support | 2024 |
| Bebstar Developments Ltd | Professional & scientific | 2025 |
| Because I CAN Fitness Limited | Other services | 2019 |
| Beckford & Boyt Mobile Autocare Ltd | Wholesale & retail | 2019 |
| Beckluxe Ltd | Wholesale & retail | 2025 |
| Beckycare Ltd | Education | 2024 |
| Bectin Limited | Financial services | 2015 |
| Bedgrove Barbers Limited | Other services | 2009 |
| Bedgrove Fish & Grill Ltd | Hospitality | 2024 |
| Bedgrove Fish Ltd | Hospitality | 2021 |
| Beechfield Restorations Limited | Other services | 2003 |
| Beef King Aylesbury Ltd | Hospitality | 2025 |
| BEENAIL1 Limited | Other services | 2022 |
| Beetle Biter Enterprises Ltd | Information & communication | 2024 |
| Beffected Ltd | Information & communication | 2023 |
| Befrelma Ltd | Professional & scientific | 2013 |
| Belgrave Swift Ltd | Wholesale & retail | 2014 |
| Beline Holdings UK Ltd | Transport & logistics | 2026 |
| Bella Pizza Aylesbury Limited | Hospitality | 2024 |
| Belleza Health and Beauty Ltd | Health & social care | 2007 |
| Belmount Properties Ltd. | Real estate | 2022 |
| Belro Limited | Other services | 2016 |
| BEN C Photography Ltd | Professional & scientific | 2023 |
| BEN Engineering Services Limited | Professional & scientific | 2016 |
| BEN Tinniswood Ltd | Arts & recreation | 2026 |
| Bencarnna Limited | Other services | 2017 |
| Benjamin Andrews Limited | Extraterritorial | 2024 |
| Bennett Mechanical Services Limited | Administrative & support | 1997 |
| Beracah Express Holding Ltd | Real estate | 2024 |
| Beracah Express Ltd | Wholesale & retail | 2013 |
| Berber Artisan Ltd | Manufacturing | 2022 |
| Berkwell Investments Limited | Real estate | 2019 |
| Bernard LEE Limited | Administrative & support | 2011 |
| Bernwode Limited | Information & communication | 2014 |
| Bernwode Rural Shed CIC | Other services | 2024 |