| Aylesbury PUB Co Ltd | Hospitality | 2020 |
| Aylesbury ROC Ltd | Other services | 2020 |
| Aylesbury Rugby Football Club Limited | Arts & recreation | 2010 |
| Aylesbury Service Centre Ltd | Wholesale & retail | 2018 |
| Aylesbury Services Ltd | Transport & logistics | 2023 |
| Aylesbury Sports Company,Limited(The) | Real estate | 1919 |
| Aylesbury Storage LLP | | 2015 |
| Aylesbury Strength and Conditioning Ltd. | Arts & recreation | 2015 |
| Aylesbury Stylish Ltd | Other services | 2025 |
| Aylesbury Techno Consultants Ltd. | Information & communication | 2013 |
| Aylesbury Tool Hire Ltd | Wholesale & retail | 2012 |
| Aylesbury Top Hand Car Wash Ltd | Administrative & support | 2022 |
| Aylesbury Trailers Ltd | Wholesale & retail | 2025 |
| Aylesbury Transport Ltd | Transport & logistics | 2024 |
| Aylesbury Tuck Ltd | Hospitality | 2023 |
| Aylesbury Tyre Trax Limited | Wholesale & retail | 2022 |
| Aylesbury United FC Limited | Arts & recreation | 2006 |
| Aylesbury Used Cars Ltd | Wholesale & retail | 2020 |
| Aylesbury Vale Community Church | Other services | 2011 |
| Aylesbury Vale Golf Club Limited | Arts & recreation | 2005 |
| Aylesbury Vets Ltd | Professional & scientific | 2016 |
| Aylesbury Vineyard Church Ltd | Other services | 1997 |
| Aylesbury Wash Cars N Sales Ltd | Wholesale & retail | 2026 |
| Aylesbury Windows Company Limited | Construction | 2004 |
| AYLESBURY24HOURMOBILETYRES Limited | Wholesale & retail | 2025 |
| Aylescove Business Computer Developments Limited | Professional & scientific | 1985 |
| Ayra Technologies Limited | Information & communication | 2025 |
| Ayvent Limited | Construction | 2014 |
| AZ123 Medical Limited | Real estate | 2018 |
| Azori Ltd | Professional & scientific | 2021 |
| Aztec Gold Financial Services Ltd | Extraterritorial | 2012 |
| B & a Immigration Ltd | Administrative & support | 2015 |
| B & JAY Estate Limited | Real estate | 2026 |
| B & S Property Management Limited | Real estate | 2010 |
| B C Glass Ltd | Construction | 2026 |
| B C Glazing Ltd | Construction | 2024 |
| B J Mcelroy Ltd | Health & social care | 2009 |
| B Janes Services Limited | Construction | 2018 |
| B Lopez Ltd | Wholesale & retail | 2005 |
| B M Property Consult Ltd | Real estate | 2019 |
| B P Fencing Limited | Administrative & support | 2019 |
| B U Care Services Ltd | Health & social care | 2023 |
| B&B Wholesale Limited | Wholesale & retail | 2012 |
| B-Hive Associates Limited | Professional & scientific | 2016 |
| B-Tech Air Conditioning Ltd | Construction | 2026 |
| B-Well Group of Companies Ltd | Real estate | 2024 |
| B-Well Health & Social Care Group Limited | Health & social care | 2012 |
| B-Wise Medical Limited | Health & social care | 2025 |
| B. G. Racing Ltd | Arts & recreation | 2007 |
| B.A.P Transport Limited | Transport & logistics | 2025 |
| B.Aspired Ltd | Wholesale & retail | 2023 |
| B.C. Electrical Techniques Limited | Manufacturing | 1950 |
| B.F International Holding Ltd | Real estate | 2020 |
| B.G. Richardson & Sons LLP | | 2004 |
| B.J.Kalan Limited | Wholesale & retail | 2019 |
| B.S.Building&Landscaping Ltd | Construction | 2012 |
| B.V. (Bellvano) Atelier Ltd | Manufacturing | 2025 |
| B.W. Aylesbury (Holdings) Limited | Financial services | 2016 |
| B.W. Aylesbury Limited | Wholesale & retail | 1996 |
| B2BPM Ltd | Real estate | 2024 |
| B3 Commercial Services Ltd | Wholesale & retail | 2022 |
| Baag Civil Engineering Limited | Professional & scientific | 2023 |
| Baansuan Limited | Administrative & support | 2023 |
| Baara - Gold Limited | Mining & quarrying | 2024 |
| Baby Swimming Milton Keynes and Northampton LLP | | 2023 |
| Bacchus Intuitive Consultancy Limited | Professional & scientific | 2025 |
| Backstreet Barbers Ltd | Other services | 2024 |
| Backtofront Software Ltd | Wholesale & retail | 2007 |
| BAD Taste Recordings Limited | Arts & recreation | 2011 |
| Badgers SET Ltd | Hospitality | 2019 |
| Badr Consulting Limited | Professional & scientific | 2017 |
| Baggio Limited | Transport & logistics | 2017 |
| Bailey-Kennedy Properties Limited | Real estate | 2007 |
| Baileys Total Concept Limited | Wholesale & retail | 1998 |
| Baje Properties Limited | Real estate | 2000 |
| Bajwa Soltech Ltd | Information & communication | 2020 |
| Bakerbuildings Ltd | Construction | 2020 |
| Bakersfield Construction Limited | Construction | 2006 |
| Balaju Height Limited | Wholesale & retail | 2020 |
| Baldwin's Bathrooms Ltd | Construction | 2022 |
| Ball Consulting Ltd | Professional & scientific | 2016 |
| Ballgate Limited | Information & communication | 1998 |
| Ballinger Workshop Ltd | Construction | 2025 |
| Balloonmania Ltd | Wholesale & retail | 2017 |
| Balsall Common Ltd | Real estate | 2024 |
| Bambaru Limited | Information & communication | 2022 |
| Bambi Pictures Limited | Information & communication | 2022 |
| Bambile Ltd | Professional & scientific | 2019 |
| Bancci Design Limited | Extraterritorial | 2024 |
| Band Consulting Services Limited | Professional & scientific | 2004 |
| Banksia Consulting Ltd | Professional & scientific | 2025 |
| Banno G Limited | Wholesale & retail | 2025 |
| Banwell Ltd | Wholesale & retail | 2021 |
| Banyan Housing Ltd | Real estate | 2023 |
| Bar Noosh Ltd | Hospitality | 2019 |
| Bardrake Limited | Real estate | 1972 |
| Barefoot and Back to Nature Ltd | Information & communication | 2023 |
| Bargain Megastore Ltd | Wholesale & retail | 2012 |
| Bargain Sales Ltd | Wholesale & retail | 2025 |
| Bargainmania Limited | Wholesale & retail | 2019 |