| ST Andrews Executive Limited | Administrative & support | 2026 |
| ST Ayles Skiff International Ltd | Arts & recreation | 2021 |
| ST Monans Auld Kirk Enterprise | Other services | 2024 |
| ST Monans Minimarket Limited | Wholesale & retail | 2015 |
| ST. Andrews Holiday Services Ltd | Administrative & support | 2018 |
| Stenton Farming Company | | 1991 |
| Steven Wood Fresh Fish Ltd | Hospitality | 2020 |
| Stewart & Parsons Limited | Administrative & support | 2005 |
| Stocks Events Limited | Hospitality | 2015 |
| Stoneflag Limited | Real estate | 2024 |
| Strikeforce Security Services UK Ltd | Administrative & support | 2026 |
| Styleup NOW Limited | Other services | 2019 |
| Supernova Cleaning Service Ltd | Administrative & support | 2022 |
| T & K Brown Ltd. | Health & social care | 2011 |
| Tabletop Scotland Limited | Administrative & support | 2019 |
| Tamen Convenimus Limited | Health & social care | 2026 |
| Tartan Hospitality Limited | Hospitality | 2019 |
| THE Crail Community Larder CIC | Hospitality | 2024 |
| THE Laughing Stock Food Limited | Hospitality | 2010 |
| THE Pittenweem Harbour Company Limited | Hospitality | 1997 |
| THE Scottish Fisheries Museum Trust Limited | Arts & recreation | 1968 |
| THE WEE Couper of Fife Ltd | Wholesale & retail | 2018 |
| Tidy Cleaner Ltd | Administrative & support | 2018 |
| Tour Worx Ltd | Education | 2024 |
| Trading With MC Ltd | Wholesale & retail | 2026 |
| Trusted Babysitter Scotland Ltd | Health & social care | 2025 |
| Twoticks Limited | Education | 2008 |
| Unique Cleans & Kitouts Ltd | Administrative & support | 2025 |
| Veloceam Ltd | Education | 2012 |
| Ventures (Fife) Limited | Manufacturing | 2012 |
| W Jardine Ltd | Wholesale & retail | 2020 |
| WEE Dude Racing Ltd | Agriculture & fishing | 2025 |
| Westgate Studios Ltd | Real estate | 2024 |
| Winaway Ltd | Agriculture & fishing | 2018 |
| WOK & Spice East Fife Ltd | Hospitality | 2025 |
| Wwds (Scotland) Limited | Manufacturing | 2014 |