| Greig Woodward Catering Limited | Hospitality | 2025 |
| Guide US Breaks Ltd | Real estate | 2026 |
| Guide US Cleaning Ltd | Administrative & support | 2026 |
| H & R Productions Limited | Arts & recreation | 2025 |
| Handy Services (Fife) Ltd | Health & social care | 2017 |
| Harbour Food Shop ST Monans Ltd | Wholesale & retail | 2025 |
| Headlessacting Ltd | Arts & recreation | 2025 |
| Healy Communications Ltd | Professional & scientific | 2016 |
| Helen Pryde Limited | Professional & scientific | 2016 |
| Hempton Consulting Ltd | Construction | 2024 |
| Highlander Spirits Company Ltd | Manufacturing | 2020 |
| Hollytech Consulting Limited | Information & communication | 2014 |
| Honestas Maritime Limited | Professional & scientific | 1996 |
| House of Brìghde Ltd | Wholesale & retail | 2025 |
| HTH Corporation Limited | Health & social care | 2026 |
| I & L Rooney Ltd | Financial services | 2020 |
| Ianoremac Limited | Other services | 2016 |
| Inclusiq Ltd | Professional & scientific | 2013 |
| Isle of MAY Boat Trips Limited | Transport & logistics | 2013 |
| J B Anderson Fresh Fish Ltd | Wholesale & retail | 2020 |
| JG Intl Ltd | Professional & scientific | 2024 |
| Jo-Jo's Hostels Ltd | Hospitality | 2016 |
| Junction Building Limited | Construction | 2021 |
| K E C Fife Ltd | Administrative & support | 2026 |
| K M Duncan Agricultural Engineering Limited | Financial services | 2013 |
| K9 Grooming Ltd | Other services | 2018 |
| Kennard Investments Ltd | Real estate | 2023 |
| Kilrenny Mill Coastal Escapes Ltd | Hospitality | 2021 |
| Kingdom Maritime Ltd | Professional & scientific | 2025 |
| Kitchen Improvements Limited | Construction | 2019 |
| Kontrotech Limited | Manufacturing | 1993 |
| L'Obsteria Ltd | Agriculture & fishing | 2026 |
| Laine Estates Limited | Financial services | 2024 |
| LED Cleaning Services Ltd | Administrative & support | 2017 |
| LED Laundry Services Ltd | Administrative & support | 2023 |
| LEE Johnstone Roofing & Plastering Limited | Construction | 2015 |
| Local Heroes Marketing Ltd | Professional & scientific | 2024 |
| Luna Rossa Restaurant Limited | Hospitality | 2025 |
| MA Ground Maintenance Ltd | Administrative & support | 2025 |
| MA Property East Neuk Ltd | Real estate | 2024 |
| Madeira in Fife Events Ltd | Other services | 2025 |
| Marc Horsburgh Ltd | Wholesale & retail | 2022 |
| MC Fratm Ltd | Manufacturing | 2025 |
| Meldrum Interiors Ltd | Wholesale & retail | 2017 |
| Metaflake Limited | Manufacturing | 1996 |
| Metaflake Trustee Company Limited | Professional & scientific | 2025 |
| Mhairi Mackenzie Associates Ltd. | Professional & scientific | 2012 |
| Milotinova Sunshine Cleaning Company Ltd | Administrative & support | 2024 |
| Monkfish Ventures Ltd | Professional & scientific | 2014 |
| Murray Library | Hospitality | 2015 |
| New Bruce Properties Ltd | Real estate | 2024 |
| NG Fife Properties Ltd | Hospitality | 2025 |
| NG Property Management Ltd | Real estate | 2015 |
| Nice Code Ltd | Information & communication | 2020 |
| Njmiller Ltd | Wholesale & retail | 2020 |
| Norness Shellfish Ltd | Agriculture & fishing | 2025 |
| Of-Officine Fuoriserie Whisky Atelier Limited | Manufacturing | 2023 |
| Old Marygate Pittenweem Ltd | Arts & recreation | 2025 |
| Old TOM GIN Company in ST Andrews Ltd | Manufacturing | 2017 |
| OTG 1821 ST Andrews Limited | Manufacturing | 2020 |
| Ovenstone 109 Limited | Manufacturing | 2017 |
| P & E Allan Fresh Fish Ltd | Wholesale & retail | 2021 |
| Paddleactive Limited | Education | 2016 |
| Peddie Engineering Ltd | Administrative & support | 2021 |
| Phoenix UTD GFC Limited | Arts & recreation | 2025 |
| Physiotherapy Links Ltd | Health & social care | 2015 |
| Pittenweem Fish House Limited | Wholesale & retail | 2021 |
| Pittenweem Tennis Club Ltd | Arts & recreation | 2016 |
| Plumbing Services Ltd | Construction | 2018 |
| Premier Stays Fife Ltd | Administrative & support | 2022 |
| Prospect Hill Design Limited | Professional & scientific | 2013 |
| Purity Domestic Cleaning Ltd | Administrative & support | 2025 |
| R & K Holiday Properties Ltd | Hospitality | 2020 |
| R & K Properties (Scotland) Ltd | Real estate | 2014 |
| Raith Construction Limited | Construction | 2025 |
| Renaq Ltd | Agriculture & fishing | 2026 |
| Rhubarb Park Limited | Hospitality | 2025 |
| RKF Poultry Ltd | Agriculture & fishing | 2017 |
| Robertson Hospitality Limited | Hospitality | 2023 |
| Robinson Crusoe (Fife) Ltd | Agriculture & fishing | 2011 |
| Royal Burgh of Pittenweem Arts Festival | Arts & recreation | 2002 |
| SAM by the Sea Ltd | Wholesale & retail | 2022 |
| Sandy's Fruit & VEG Ltd | Wholesale & retail | 2025 |
| Scabby Queen Music Ltd | Information & communication | 2023 |
| Scotsaunas Ltd | Other services | 2024 |
| Scott Process Technology Ltd. | Administrative & support | 1995 |
| Seaforth Seafood Ltd | Administrative & support | 2018 |
| Seed to Plate Ltd | Hospitality | 2023 |
| Selkie Minis Ltd | Manufacturing | 2023 |
| Shoregate Hospitality Ltd. | Hospitality | 2019 |
| Simmons Engineering Services Ltd | Administrative & support | 2018 |
| Sitheansona Limited | Hospitality | 2003 |
| Smhconsultancy Ltd | Professional & scientific | 2019 |
| Solutions Consultancy Limited | Professional & scientific | 1994 |
| Sophie JG Ltd | Other services | 2021 |
| Spindrift (Guesthouse) Ltd | Hospitality | 2022 |
| Spokes Cycles Ltd. | Wholesale & retail | 2010 |
| Sprinkler Solutions Ltd | Construction | 2018 |
| ST Andrews Brushworks Ltd | Construction | 2025 |
| ST Andrews Cleaning and Laundry Services Ltd | Administrative & support | 2025 |