| Aberdour Property Developments Ltd | Real estate | 2025 |
| Acre Engineering Services Ltd | Manufacturing | 2022 |
| Aeble Cider Shop Limited | Wholesale & retail | 2021 |
| Afrodysia Beverage Group Ltd | Wholesale & retail | 2015 |
| Ainsdale Shellfish Limited | Agriculture & fishing | 2016 |
| Anaesthetic Expert Witness Ltd | Professional & scientific | 2021 |
| Anchor Point Properties Limited | Real estate | 2024 |
| Anna Carolina LLP | | 2017 |
| Anstruther Foods Ltd | Hospitality | 2023 |
| Anstruther Trading Co Limited | Hospitality | 2019 |
| Argyll GT Craft Cars Limited | Manufacturing | 2021 |
| Arncroach and Carnbee Community Development Trust | Other services | 2011 |
| Astute Fire (Scotland) Ltd | Professional & scientific | 2010 |
| Balcomie Holdings Ltd | Real estate | 2026 |
| Balcomie Links Caravan Park Ltd | Hospitality | 2018 |
| Balcomie Tide Ltd | Wholesale & retail | 2025 |
| Balmonth Energy Limited | Energy | 2013 |
| Bear Solutions Scotland Ltd | Construction | 2024 |
| BGG Foods Ltd | Hospitality | 2025 |
| Big Blue Boat Ltd | Professional & scientific | 2017 |
| Big KEV Ltd | Professional & scientific | 2026 |
| Big Yellow Taxi Co (Scotland) Ltd | Transport & logistics | 2021 |
| Bino Ventures Ltd | Hospitality | 2021 |
| Bonerbo Farm Ltd | Agriculture & fishing | 2023 |
| Bonerbo Luxury Lodges Limited | Hospitality | 2018 |
| Bonn Pizza Ltd | Hospitality | 2026 |
| BSD UK Holdings Limited | Real estate | 2021 |
| Cafe Malo Ltd | Hospitality | 2024 |
| Caiplie Properties Limited | Hospitality | 2023 |
| Calypso's Cabs Ltd | Transport & logistics | 2022 |
| Carousel Property Investments Limited | Construction | 2024 |
| Carpet Sun Limited | Wholesale & retail | 2019 |
| CCA Power Limited | Energy | 2015 |
| Charis Foundation | Health & social care | 2006 |
| Chipahoy Larachmor Ltd | Hospitality | 2024 |
| Choice Economists Ltd | Professional & scientific | 2022 |
| CML Chip Shops Ltd | Hospitality | 2024 |
| CML Inns Ltd | Hospitality | 2024 |
| CML Restaurants Ltd | Hospitality | 2024 |
| Code Bunnies Ltd | Information & communication | 2023 |
| Coft Ltd | Hospitality | 2017 |
| Consulted Ltd | Professional & scientific | 2007 |
| Corelink HV Ltd | Construction | 2026 |
| Cornceres Generating Limited | Real estate | 2015 |
| Crab Publishing | Information & communication | 2016 |
| Craignish Consultancy Ltd | Professional & scientific | 2022 |
| Crail Ceramics Ltd | Manufacturing | 2017 |
| Crail Golfing Society Limited | Arts & recreation | 2022 |
| Crail Pharmacy Limited | Health & social care | 2012 |
| Creative Calm Community Ltd | Education | 2024 |
| Croga Consulting Ltd | Professional & scientific | 2017 |
| CS & CC Limited | Hospitality | 2022 |
| Curativate Limited | Education | 2019 |
| Cycle Ikot Ltd | Wholesale & retail | 2023 |
| D Stewart Landscapes Ltd | Construction | 2024 |
| Daniels Access Ltd | Construction | 2022 |
| David Deans Limited | Manufacturing | 2019 |
| David Shepherd Builder Ltd | Construction | 2023 |
| Different Voice Limited | Arts & recreation | 1995 |
| DJP Property Services Ltd | Real estate | 2019 |
| Doge Limited | Information & communication | 2025 |
| Dominic Ibbotson Limited | Professional & scientific | 2005 |
| Dream to Dram Tours Ltd | Administrative & support | 2024 |
| Dulcian Associates Ltd | Professional & scientific | 2021 |
| Dunbar Software Company Limited | Information & communication | 1999 |
| Dunperrogh Investments Ltd | Hospitality | 2018 |
| East Coast Laundry Service Ltd | Other services | 2020 |
| East Neuk Campervans Ltd. | Manufacturing | 2004 |
| East Neuk Centre Trust | Education | 2010 |
| East Neuk Cleaners Ltd | Administrative & support | 2021 |
| East Neuk Escapes Ltd | Real estate | 2026 |
| East Neuk Fish Merchants Ltd | Hospitality | 2020 |
| East Neuk Fishing Company Ltd | Agriculture & fishing | 2023 |
| East Neuk Joinery & Construction Ltd | Construction | 2020 |
| East Neuk Knits LLP | | 2025 |
| East Neuk Laundrette Ltd | Other services | 2021 |
| East Neuk Salt Co. Limited | Hospitality | 2019 |
| EJM Consultancy Ltd | Professional & scientific | 2021 |
| Elie Select Property Management Limited | Real estate | 2017 |
| Espressowsky Italian Espresso Martini Limited | Manufacturing | 2021 |
| Falkland Kitchen Farm Limited | Agriculture & fishing | 2014 |
| FAT Stock Group Limited | Hospitality | 2015 |
| Feta Street Food Ltd | Hospitality | 2021 |
| FHL Client Services Ltd | Administrative & support | 2021 |
| Fife Architects LLP | | 2024 |
| Fife Coast Properties Ltd | Real estate | 2025 |
| Fife Holiday Lettings Ltd | Hospitality | 2016 |
| Fife Shoreline Cleaning Ltd | Administrative & support | 2026 |
| FMB Structural Design Limited | Professional & scientific | 2003 |
| Fochabers Chippy Ltd | Hospitality | 2018 |
| Fortune House Cellardyke Limited | Hospitality | 2024 |
| Full Properties Limited | Real estate | 2013 |
| G Dyce Autos Ltd | Wholesale & retail | 2026 |
| G H Barnett & SON Ltd | Professional & scientific | 2015 |
| Gary White OIL & GAS Heating Services Ltd | Construction | 2020 |
| Gmjn Engineering Ltd | Professional & scientific | 2024 |
| Golfinscotland.De Ltd | Administrative & support | 2018 |
| Graeme Mcintosh Limited | Wholesale & retail | 2013 |
| Grason Properties Limited | Construction | 2023 |
| Gray & Pringle Limited | Wholesale & retail | 1928 |