| A & D Marine Solutions Ltd | Administrative & support | 2024 |
| A 2 BEE Travel Limited | Transport & logistics | 2026 |
| A Golden Touch Ltd | Administrative & support | 2025 |
| A J Gunning Limited | Health & social care | 2015 |
| A M Shuttleworth Ltd | Health & social care | 2022 |
| A.F. Scotland Limited | Professional & scientific | 1990 |
| A1 Graphics Ltd | Manufacturing | 2019 |
| AAA Electrical Ltd | Construction | 2019 |
| Abbfinn Limited | Extraterritorial | 2024 |
| AC Dairies Limited | Manufacturing | 2021 |
| Adam Carlyle Brickwork Limited | Construction | 2016 |
| AEF Enterprises Ltd | Wholesale & retail | 2024 |
| Agri Cleaning Solutions Ltd | Administrative & support | 2022 |
| Airmix Technologies Limited | Extraterritorial | 2010 |
| Albie Forestry Limited | Agriculture & fishing | 2010 |
| Albie Holdings Limited | Financial services | 2023 |
| ALL Current Electrics Ltd | Construction | 2025 |
| Allan Mckenzie Limited | Agriculture & fishing | 2019 |
| Allanton Peace Shop Ltd | Wholesale & retail | 2020 |
| Allsteady Ltd | Information & communication | 2022 |
| Almond View Limited | Administrative & support | 2021 |
| Amber Programmable Design Limited | Manufacturing | 1996 |
| AMI Mcquarrie Consulting Ltd | Professional & scientific | 2022 |
| Annan Bodybuilding Gym Ltd | Arts & recreation | 2026 |
| Annan Dental Practice Limited | Health & social care | 2015 |
| Annan Enterprises Ltd | Real estate | 2024 |
| Annan Nails & Beauty Ltd | Other services | 2023 |
| Annan Podiatry Limited | Health & social care | 2023 |
| Annan Properties Limited | Real estate | 2019 |
| Annan Taxi Co Ltd | Transport & logistics | 2022 |
| Annan Wattbike Studio Ltd | Arts & recreation | 2016 |
| Annandale and Nithsdale Community Benefit Company Limited | Administrative & support | 2014 |
| Annandale Distillery Company Limited | Manufacturing | 2007 |
| Annandale Herald Limited | Extraterritorial | 2009 |
| Annerley Properties Limited | Real estate | 2017 |
| Asbestos Advice, Consultancy and Training Ltd | Professional & scientific | 2018 |
| Auld and Cairn Ltd | Professional & scientific | 2025 |
| Barrasgate Farm | | 1987 |
| Barry's Butchers Limited | Wholesale & retail | 2023 |
| Bc&V Nicholson Ltd | Agriculture & fishing | 2018 |
| Beacom Farms Limited | Agriculture & fishing | 1993 |
| Beattie & Wilson Ltd | Professional & scientific | 2025 |
| Beattie Land Limited | Real estate | 2026 |
| Bespoke Resorts Ltd. | Professional & scientific | 2005 |
| Blackburn Farm | | 1986 |
| BNR Motorworx Ltd | Wholesale & retail | 2021 |
| Border Hospitality Ltd | Hospitality | 2010 |
| Border Reivers Tweed Company Limited | Extraterritorial | 2020 |
| Brandedleys Country Park Limited | Hospitality | 2025 |
| Brickwrx Limited | Construction | 2020 |
| Broadmeadow Caravans Ltd | Transport & logistics | 2019 |
| Broom Leisure Limited | Agriculture & fishing | 2005 |
| Bryson Meats Limited | Professional & scientific | 1977 |
| Butler Land Management Ltd | Real estate | 2014 |
| C & K Property Rentals Ltd | Real estate | 2025 |
| C & KW Holdings Limited | Financial services | 2021 |
| C Bell Property Management Limited | Real estate | 2017 |
| C Woodman Engineering Limited | Professional & scientific | 2009 |
| Calcomms Limited | Information & communication | 2015 |
| Caledonia Ice Cream Ltd | Hospitality | 2023 |
| Cameron Grieve Transport Limited | Transport & logistics | 2017 |
| Candy Crush Co Ltd | Wholesale & retail | 2025 |
| Captured by Carrie Limited | Professional & scientific | 2013 |
| Care 4 YOU (D&G) Limited | Health & social care | 2023 |
| Carpetbay Ltd | Other services | 2017 |
| Caspian Annan Ltd | Hospitality | 2026 |
| CB Shellfish Ltd | Agriculture & fishing | 2013 |
| CC Nicholson Ltd | Agriculture & fishing | 2023 |
| Charisma Properties Scotland Ltd | Real estate | 2023 |
| China House (Gretna Green) Limited | Hospitality | 2022 |
| CJJ Rascal Ltd | Financial services | 2022 |
| Clampuk Ltd | Professional & scientific | 2022 |
| CMP2 Energy Limited | Professional & scientific | 2026 |
| CN Investments Ltd | Real estate | 2024 |
| Cochran Group Ltd | Professional & scientific | 2010 |
| Cochran Ltd | Manufacturing | 2010 |
| Cochran Services Ltd | Manufacturing | 2010 |
| Comlongon Castle Ltd | Hospitality | 2021 |
| Comlongon Estate Limited | Arts & recreation | 2010 |
| Comlongon Ltd | Hospitality | 2021 |
| Comm Couragous Ltd | Agriculture & fishing | 2016 |
| Cordley Investments Ltd | Financial services | 2021 |
| CPC Electrical Contractors Limited | Construction | 2003 |
| Craig Conroy Ltd | Transport & logistics | 2025 |
| Crossaig Estate LLP | | 2021 |
| Cutting Edge Joinery (Annan) Ltd | Construction | 2022 |
| D & G GAS Services Ltd | Construction | 2020 |
| D and G Fish Sales Ltd | Wholesale & retail | 2021 |
| D I Ventures Limited | Hospitality | 2005 |
| D R W Ltd. | Administrative & support | 2005 |
| D W Drennan Building Services Limited | Construction | 2013 |
| D. G. Heuchan Contracts Ltd | Construction | 2020 |
| D.G. Heuchan Construction Ltd. | Construction | 1998 |
| Derek Carruthers Construction Limited | Construction | 2006 |
| DFS Caskets Limited | Manufacturing | 2004 |
| Digamat Ltd | Other services | 2024 |
| Digital Solway Ltd | Information & communication | 2023 |
| Douglas Swan & Sons Limited | Other services | 2005 |
| Drove Weavers Limited | Manufacturing | 2020 |
| Drummuir Caledonia Ltd | Hospitality | 2025 |