| DRY Ice (GB) Ltd | Wholesale & retail | 2014 |
| DWS Windows Limited | Construction | 2017 |
| E&H Enterprises Ltd | Professional & scientific | 2019 |
| EA Irving Ventures Ltd | Hospitality | 2022 |
| Earlfields Property & Lettings Ltd | Hospitality | 2023 |
| Ecclefechan Hotel Ltd | Hospitality | 2024 |
| Eco Workspace Limited | Administrative & support | 2018 |
| Eco-Axess Limited | Financial services | 2020 |
| Eco-Genics (Holdings) Ltd | Financial services | 2014 |
| Eco-Genics Ltd | Manufacturing | 2009 |
| Eco-Process Solutions Limited | Other services | 2019 |
| Electrical Install & Commissioning (Ei&C) Ltd | Manufacturing | 2025 |
| Ellsdenn Ltd | Hospitality | 2025 |
| Enhance Aesthetics by Paula Ltd | Health & social care | 2021 |
| Enkey Limited | Extraterritorial | 2023 |
| Euro Mini Market (Annan) Limited | Wholesale & retail | 2024 |
| Evans Hobby and Tech Limited | Wholesale & retail | 2021 |
| Fabrication & Maintenance Solutions Ltd | Manufacturing | 2018 |
| Fechan Naturals Ltd | Wholesale & retail | 2025 |
| Feel Safe North Limited | Administrative & support | 2011 |
| Fiona Smart Coaching & Consultancy Limited | Education | 2022 |
| Firth Veterinary Centre Limited | Professional & scientific | 2013 |
| Flooring Scotland Ltd | Wholesale & retail | 2013 |
| Fornieco (Scotland) Ltd | Wholesale & retail | 2019 |
| G & a Macdonald Limited | Construction | 2006 |
| G Cairns Properties Ltd | Real estate | 2024 |
| Gavin Bell Mechanical Engineering Limited | Professional & scientific | 2018 |
| GBG Environmental Ltd | Construction | 2019 |
| Georg LOU N Ltd | Agriculture & fishing | 2005 |
| George Stewart Construction Limited | Construction | 2020 |
| Glenbrae Limited | Administrative & support | 2019 |
| Glencartholm Holidays Ltd | Hospitality | 2025 |
| Glentarras Distillery Limited | Extraterritorial | 2021 |
| GLW Enterprises Limited | Hospitality | 2019 |
| Gough Engineering Limited | Professional & scientific | 2026 |
| Graeme Anderson Consulting Limited | Professional & scientific | 2022 |
| Graham Mechanical and Fabrication Limited | Professional & scientific | 2023 |
| Greenfield (Annan) Limited | Agriculture & fishing | 2009 |
| Gretna Green Brides Limited | Professional & scientific | 2006 |
| GS Hogg Ltd | Construction | 2017 |
| GSM Transport (Scotland) Limited | Transport & logistics | 2025 |
| Gunning Properties Limited | Real estate | 2021 |
| Gurbax Limited | Wholesale & retail | 2002 |
| GUY Kirkpatrick Drilling & Grouting Ltd | Professional & scientific | 2026 |
| H & I Engineering Limited | Manufacturing | 1973 |
| H. K. Thorburn Design and Build Ltd | Construction | 2022 |
| Hann & Co Solicitors Ltd. | Professional & scientific | 2007 |
| Hann and Co Estate Agents Ltd | Professional & scientific | 2011 |
| Hawes Forestry Ltd | Agriculture & fishing | 2021 |
| Hayley Barnes Beauty Studio Ltd | Other services | 2024 |
| Highway Holidays Limited | Administrative & support | 2022 |
| HKA Homes Ltd | Real estate | 2025 |
| Holly Edge Limited | Administrative & support | 2016 |
| Horizon Health & Safety Ltd | Professional & scientific | 2021 |
| HV Electrical Ltd | Energy | 2018 |
| Iain Carruthers Construction Limited | Construction | 2004 |
| ICM Developments Limited | Construction | 2007 |
| Irish Republican Art Ltd | Professional & scientific | 2025 |
| Irving Engineering Services Ltd | Transport & logistics | 2019 |
| Irvwood Ltd | Real estate | 2019 |
| J N Heating Ltd | Construction | 2020 |
| J N Home Investments Ltd | Real estate | 2022 |
| J W Logistics Scotland Ltd | Transport & logistics | 2021 |
| J&R Manufacturing Ltd | Wholesale & retail | 2024 |
| J.W. Galloway Limited | Manufacturing | 1935 |
| James M Stuart Haulage Limited | Transport & logistics | 2019 |
| Jamieson Brothers Online Ltd | Wholesale & retail | 2020 |
| Jandera Properties Limited | Real estate | 2026 |
| Jaym Property Ltd | Real estate | 2025 |
| JC Parcels Ltd | Transport & logistics | 2026 |
| Jftass Ltd | Administrative & support | 2026 |
| JKB Property and Holiday Lets Ltd | Real estate | 2023 |
| JMT Engineering (Scotland) Limited | Manufacturing | 2017 |
| Joe's Newsagents Ltd | Hospitality | 2019 |
| John Roddick & SON (Solicitors) Ltd. | Professional & scientific | 2009 |
| Johnstoneleas Properties Limited | Construction | 2004 |
| Jukes Cars Ltd | Transport & logistics | 2026 |
| Karizma Turkish Barber Shop Ltd | Other services | 2022 |
| Karma Delivery Ltd | Transport & logistics | 2025 |
| KC Invesco Limited | Professional & scientific | 2019 |
| Kddd Enterprises Ltd | Other services | 2025 |
| Keith Earl Joinery Limited | Construction | 2017 |
| Kelbray Ventures Ltd | Hospitality | 2022 |
| Kelmar Contracting Ltd | Agriculture & fishing | 2021 |
| Kev's Automotive Ltd | Wholesale & retail | 2025 |
| Key Traders Ltd | Wholesale & retail | 2026 |
| Kippford Consulting Limited | Administrative & support | 2018 |
| KMD Contracting Limited | Administrative & support | 2023 |
| KTS Racing Limited | Professional & scientific | 2022 |
| KY Building Ltd | Construction | 2020 |
| L Murray Joinery Ltd | Manufacturing | 2025 |
| L Murray Property Limited | Real estate | 2020 |
| L'Atelier Laser Products Limited | Wholesale & retail | 2021 |
| L.Clark Engineering Ltd | Professional & scientific | 2026 |
| Lactive Limited | Arts & recreation | 2025 |
| Lakemast Limited | Real estate | 2001 |
| Langholm Scarf Company Limited | Extraterritorial | 2020 |
| Laser Juice Limited | Wholesale & retail | 2019 |
| Latimer Financial Services Limited | Financial services | 2012 |
| LEE Dixon Groundworks Ltd | Construction | 2022 |