| ALL Malvern Project Ltd | Construction | 2025 |
| Amlwch Community Festival | Other services | 2018 |
| Amlwch Cycles Ltd | Other services | 2023 |
| Amlwch Dog Grooming Ltd | Other services | 2019 |
| Amlwch Limited | Hospitality | 2018 |
| Amlwch Windows Ltd | Construction | 2007 |
| Anglesey Game Farm Limited | Agriculture & fishing | 2002 |
| Anglesey Marine Services Limited | Agriculture & fishing | 2010 |
| Anglesey Mining PLC | Mining & quarrying | 1984 |
| Anglesey Turf Company Limited | Agriculture & fishing | 2008 |
| Anja Pownell Ltd | Administrative & support | 2021 |
| Arms Environmental Ltd | Water & waste | 2025 |
| Avibek Ltd | Manufacturing | 2024 |
| Axtion Ltd | Water & waste | 2020 |
| Barodtec Limited | Professional & scientific | 2021 |
| Barrett Book Keeping & Accountancy Limited | Professional & scientific | 2020 |
| BKB Fire Risk Consultants Ltd | Public administration | 2019 |
| Bluebell Nexus Ltd | Administrative & support | 2025 |
| Brothers Window Cleaning Ltd | Administrative & support | 2022 |
| Bull Bay Community Boat House CIC | Hospitality | 2024 |
| Business To-Go Limited | Information & communication | 2019 |
| Bywyd Llawn Limited | Health & social care | 2010 |
| Caemon Ltd | Construction | 2016 |
| Capel Bach Leisure Limited | Hospitality | 2021 |
| Central Garages (Amlwch) Ltd | Wholesale & retail | 2008 |
| Circuit Breaker Specialists Limited | Other services | 2019 |
| CKS Commercial Fishing Ltd | Agriculture & fishing | 2013 |
| Clare & Jones Limited | Construction | 2015 |
| Claudine Chapman Bookkeeping Services Limited | Professional & scientific | 2024 |
| Clwb Fitness Ltd | Other services | 2023 |
| Copper Kingdom Enterprises Ltd | Other services | 2025 |
| Copper MON Electrical Ltd | Construction | 2022 |
| Cure Solutions Limited | Information & communication | 2019 |
| Cwmni Cymunedol Amlwch CYF | Professional & scientific | 2016 |
| Cyfrif CYF | Professional & scientific | 2026 |
| Cysgod Y Gwynt CYF | Agriculture & fishing | 2020 |
| D. E. Griffiths Construction Limited | Construction | 2021 |
| David Peters Farms Limited | Agriculture & fishing | 1972 |
| Davies Property Investors Ltd | Construction | 2026 |
| Deepscreen Ltd | Administrative & support | 2025 |
| DLB Ltd | Administrative & support | 2025 |
| Dominic Richardson's Window Cleaning Services Limited | Administrative & support | 2022 |
| DTM Technologies Limited | Information & communication | 2014 |
| Duet Solutions Limited | Professional & scientific | 2022 |
| Ewyllys DA Amlwch | Health & social care | 2018 |
| Farley Electrical Ltd | Manufacturing | 2015 |
| First Lift Scaffolding Ltd | Construction | 2018 |
| Foodsafe Training Ltd | Professional & scientific | 2020 |
| Gardner Properties Ltd | Construction | 2004 |
| Genesis Fitness Ltd | Wholesale & retail | 2025 |
| Glyn Jones Building Contractors Ltd | Construction | 2012 |
| Godshed Productions Ltd | Education | 2025 |
| Golygfa'R Moelrhoniaid Management Ltd | Households | 2019 |
| Gretel Creates CYF | Wholesale & retail | 2018 |
| Grogan NW Ltd | Hospitality | 2021 |
| Grwp Cymunedol #Caruamlwch C.I.C. | Administrative & support | 2017 |
| H & L Skid Steer Hire Services Limited | Construction | 2016 |
| Haidd Ltd | Hospitality | 2022 |
| Halo Properties (Anglesey) Ltd | Households | 2023 |
| Hannah College Limited | Education | 2025 |
| Hannah Education and Training Limited | Education | 2025 |
| Happer-Hughes Audio Cables Ltd | Manufacturing | 2024 |
| Herbie Cranston Removals & Storage Limited | Transport & logistics | 2017 |
| Heulog Glow Limited | Wholesale & retail | 2026 |
| Hufen Y Gogledd Ltd | Manufacturing | 2020 |
| Hughes Anglesey Developments Ltd | Construction | 2025 |
| Hughes Bros. Limited | Construction | 1990 |
| Hutchinson Joinery & Building Services Ltd | Construction | 2021 |
| HUW Eurig Jones CYF | Agriculture & fishing | 2012 |
| Hyfforddiant Parys Training Limited | Education | 2003 |
| Ieybrook Limited | Professional & scientific | 1998 |
| Island Printed Products Ltd | Manufacturing | 2025 |
| J Mccann Projects Limited | Construction | 2026 |
| J P Glynn Property Development Ltd | Construction | 2024 |
| Jane Ashman Limited | Education | 2017 |
| JJ Surveys Ltd | Professional & scientific | 2026 |
| JM Building Projects Ltd | Construction | 2026 |
| Joburry Limited | Hospitality | 2008 |
| Junk Car Hire Ltd | Transport & logistics | 2026 |
| K. L Heating NW Limited | Construction | 2026 |
| KD Construction Consultancy Ltd | Professional & scientific | 2025 |
| Kinetic Experience Limited | Information & communication | 1996 |
| Korwyn Homes Limited | Real estate | 2023 |
| L & P Properties PVT Limited | Real estate | 2023 |
| Laughing Banana Enterprises Ltd | Information & communication | 2008 |
| LEE Caravan Park Limited | Hospitality | 2026 |
| Lhnm Limited | Wholesale & retail | 2018 |
| Llysdulas Estate Company Limited | Agriculture & fishing | 1954 |
| Lunar Heating & GAS Services Ltd | Professional & scientific | 2025 |
| M&G Plumbing Heating Ltd | Construction | 2026 |
| MAD Sound & Lighting Limited | Administrative & support | 2017 |
| Make IT Clean Limited | Extraterritorial | 2020 |
| Melliworks Limited | Professional & scientific | 2019 |
| Mind on Music CIC | Health & social care | 2020 |
| MON Garage Ltd | Wholesale & retail | 2021 |
| MON Power-Gen CYF. | Energy | 2025 |
| Monas Kebab Ltd | Hospitality | 2020 |
| MT Portfolio One Limited | Real estate | 2015 |
| Newall Heating & Plumbing Ltd | Construction | 2008 |
| North Haverbrook Property Services Ltd | Real estate | 2025 |