| Simply Skilled Ltd | Information & communication | 2024 |
| Simpson Software Limited | Information & communication | 2012 |
| Site Track Ltd | Wholesale & retail | 2022 |
| Sizzas Barbers Limited | Other services | 2026 |
| SJ Autos (Wessington) Ltd | Wholesale & retail | 2024 |
| SJ Shaw Limited | Financial services | 2011 |
| SJF Costing Limited | Professional & scientific | 2023 |
| SJH Double Glazing Repairs Limited | Construction | 2025 |
| SJH Property Service & Maintenance Limited | Administrative & support | 2024 |
| SJP Pristine Developments Ltd | Manufacturing | 2024 |
| Skoli Ltd | Transport & logistics | 2012 |
| Skyeland Soft Play Limited | Arts & recreation | 2016 |
| Skyline Cinema C.I.C. | Arts & recreation | 2025 |
| Slater and Myles Renewables Ltd | Construction | 2026 |
| Slater and Myles Roofing Ltd | Construction | 2022 |
| Sleetmoor Holdings Ltd | Real estate | 2025 |
| Sleetmoor United | Arts & recreation | 2001 |
| Slimming World Field Area "a" Limited | Health & social care | 1995 |
| Slimming World Field Area "B" Limited | Health & social care | 1995 |
| Slimming World Field Area "C" Limited | Health & social care | 1995 |
| Slimming World Field Area "D" Limited | Health & social care | 1995 |
| Slimming World Limited | Real estate | 1981 |
| SLS Mobile Services Ltd | Manufacturing | 2026 |
| Smart Energy Contractors Limited | Construction | 2026 |
| Smart Landscapes and Building Services Ltd | Administrative & support | 2015 |
| Smarter Impressions Limited | Administrative & support | 2016 |
| Smgas Ltd | Construction | 2022 |
| Smithurst Electrical Ltd | Construction | 2019 |
| Smpmtt Ltd | Construction | 2024 |
| Smuk Limited | Real estate | 1999 |
| SNA Food and Wine Limited | Wholesale & retail | 2025 |
| Snecks and Jumpers Limited | Construction | 2021 |
| Social Sparks Live Ltd | Professional & scientific | 2025 |
| Solid Ground Estates Ltd | Real estate | 2023 |
| Solutions 4 Tax Ltd | Professional & scientific | 2010 |
| Solutions Mobility Parts Limited | Wholesale & retail | 2025 |
| Somercotes Leisure Development Group Community Interest Company | Arts & recreation | 2007 |
| Somercotes Office Furniture Limited | Wholesale & retail | 2015 |
| Somerware Ltd | Professional & scientific | 2024 |
| Sonic Real Estate Ltd | Real estate | 2023 |
| SOR Trans Ltd | Transport & logistics | 2019 |
| Soraia Rodrigues Coaching Ltd | Administrative & support | 2023 |
| SOS Haircare Limited | Health & social care | 2018 |
| Soulcraft Services Ltd | Health & social care | 2026 |
| Soundgas Auctions Limited | Wholesale & retail | 2023 |
| Soundgas Tech Limited | Wholesale & retail | 2017 |
| Soundlab Discotheques Limited | Arts & recreation | 2003 |
| South Dakota Ltd | Other services | 2025 |
| South Normanton Bakehouse Ltd | Manufacturing | 2014 |
| South Normanton Community Church | Other services | 2000 |
| South Normanton Pre-School CIC | Education | 2009 |
| South Normanton Pubs Ltd | Hospitality | 2019 |
| South Wingfield Miners Welfare Club Limited | Hospitality | 2008 |
| Southcliffe Foods Limited | Hospitality | 2015 |
| Southcliffe Holdings Limited | Financial services | 2021 |
| Southnormanton FB Ltd | Hospitality | 2024 |
| Southside PUB Co Limited | Hospitality | 2022 |
| Southside Realty Co Ltd | Real estate | 2024 |
| Space Owls Ltd | Real estate | 2016 |
| SPC Group Holdings (UK) Limited | Financial services | 2025 |
| Specialis Limited | Administrative & support | 2026 |
| Spencer Homecare Limited | Health & social care | 2026 |
| Spencer Transport Services Ltd | Transport & logistics | 2015 |
| SPH Builders Ltd | Construction | 2016 |
| SPH Property Maintenance Limited | Administrative & support | 2018 |
| Spirit Club Ltd | Transport & logistics | 2017 |
| SPL Property Limited | Construction | 2017 |
| Spraysaway Limited | Construction | 2023 |
| Squeaky Clean Cleaning Derbyshire Limited | Administrative & support | 2023 |
| SS Safety Limited | Professional & scientific | 2018 |
| Staffi Trading Ltd | Professional & scientific | 2020 |
| Starr & Co Accounting & Bookkeeping Limited | Professional & scientific | 2025 |
| Stickers and That Limited | Wholesale & retail | 2018 |
| Stiln Holdings Ltd | Other services | 2021 |
| Stiln Ltd | Other services | 2021 |
| Stoad Limited | Other services | 2022 |
| Stone Arch Estates Ltd | Real estate | 2025 |
| Stone Planning Services Limited | Professional & scientific | 2016 |
| Stone Plant & Hydraulic Services Ltd | Manufacturing | 2019 |
| Stonebroom Community Hub (for Outdoor and Occupational Learning) CIC | Education | 2025 |
| Stonebroom Storage Solutions Ltd | Administrative & support | 2022 |
| Storm Care Solutions Limited | Manufacturing | 2004 |
| Straight Record Ltd | Information & communication | 2011 |
| Stratacore Developments Ltd | Construction | 2026 |
| Strategic Corporate Finance Limited | Extraterritorial | 2014 |
| Strategic Corporate Finance Transactions Limited | Professional & scientific | 2004 |
| Strategic Land Europe (Land Solutions for the Distribution Industry) Ltd | Transport & logistics | 2008 |
| Stretton House Limited | Real estate | 2015 |
| Stretton Scientific Limited | Wholesale & retail | 2010 |
| Stringer Surveying Services Ltd | Construction | 2003 |
| Stripe Developments Ltd | Construction | 2021 |
| Studco Limited | Information & communication | 2026 |
| Studio Peter John Ltd | Manufacturing | 2021 |
| Studymate Ltd | Professional & scientific | 2025 |
| Subframes (Holdings) Limited | Manufacturing | 2025 |
| Subframes UK Limited | Manufacturing | 2003 |
| Subtleaesthetics Ltd | Health & social care | 2025 |
| Summit Creative Ltd | Information & communication | 2021 |
| Sunny B’S Derbyshire Ltd | Other services | 2025 |
| Sunny Day Investments Ltd | Real estate | 2020 |