| Roberts Electrical Commissioning Limited | Construction | 1998 |
| Rock & Booze Limited | Hospitality | 2007 |
| Rockcentral Ltd | Professional & scientific | 2020 |
| Rockfall North America Limited | Manufacturing | 2020 |
| Rockfall UK Limited | Manufacturing | 1997 |
| Rodway Properties (Gloucs) Ltd | Real estate | 2024 |
| Roman Ridge Roofing & Building Supplies Ltd | Wholesale & retail | 2016 |
| Roofing East Midlands Limited | Construction | 2024 |
| Rooksby Roofing Group Holdings Limited | Financial services | 2018 |
| Roostridge Holdings Limited | Financial services | 2024 |
| Rose Property (Nottinghamshire) Ltd | Real estate | 2023 |
| Ross Andrew Ltd | Wholesale & retail | 2015 |
| Rotations Limited | Wholesale & retail | 2025 |
| Rotrex Group Limited | Administrative & support | 1973 |
| Royce Wood Tiles Limited | Manufacturing | 2003 |
| Rozel Health Ltd | Administrative & support | 2025 |
| RP3 Rowing Ltd | Wholesale & retail | 2017 |
| RTS Lifts Ltd | Manufacturing | 2025 |
| Rudolf Chemicals Limited | Manufacturing | 1979 |
| Ruhi TJ Ltd | Wholesale & retail | 2017 |
| Ruk-Bikes Ltd | Wholesale & retail | 2024 |
| Russ Jacques Tiling Ltd | Construction | 2019 |
| RWP Transport Ltd | Transport & logistics | 2016 |
| RYB Group Ltd | Professional & scientific | 2023 |
| Ryde Sidecar Limited | Manufacturing | 2019 |
| S + AS Limited | Wholesale & retail | 1990 |
| S Atkins Properties Ltd | Real estate | 2025 |
| S Byard Contracting Ltd | Construction | 2017 |
| S Millington Investments Ltd | Real estate | 2026 |
| S&M Retail Derby Limited | Wholesale & retail | 2022 |
| S-Tech Auto Diagnostics Ltd | Wholesale & retail | 2026 |
| S. and S. Plastics Limited | Extraterritorial | 1986 |
| S.J.Lum WAI Limited | Manufacturing | 2016 |
| S.T.M. Derby Ltd | Wholesale & retail | 2014 |
| Sabb Logistics Ltd | Transport & logistics | 2022 |
| Sabre Analytics Ltd | Information & communication | 2026 |
| Safe Edge Consulting Ltd | Professional & scientific | 2025 |
| Safety Vehicle Hire & Lease Ltd | Administrative & support | 2005 |
| Saleserve Limited | Hospitality | 1999 |
| Salience Group Ltd | Information & communication | 2026 |
| SAM Noon Limited | Wholesale & retail | 2004 |
| SAN and Lima Limited | Other services | 2026 |
| Sanam Spices Ltd | Hospitality | 2026 |
| Sandhills Leisure Vans Ltd | Manufacturing | 2025 |
| Sarah Davies OLY Ltd | Education | 2018 |
| Sarb Courier Services Limited | Transport & logistics | 2026 |
| Saunders Traffic Services Ltd | Construction | 2020 |
| Saving and Spending Savvy With Sylvie Limited | Professional & scientific | 2025 |
| Sayers Social Ltd | Professional & scientific | 2024 |
| SC Automotive Solutions Ltd | Wholesale & retail | 2026 |
| Scend (UK) Ltd | Transport & logistics | 2018 |
| Scott's Pantry Ltd | Hospitality | 2014 |
| Scotts Heavy Haulage (Ireland) Limited | Transport & logistics | 1994 |
| Sea of Spice Ltd | Hospitality | 2020 |
| Seaski UK Ltd | Wholesale & retail | 2014 |
| Seb’S Fish Room Ltd | Wholesale & retail | 2022 |
| Second Life Jewellery Ltd | Wholesale & retail | 2026 |
| Secur-It Integrated Services Ltd | Administrative & support | 2017 |
| Secur-It Litigation Funding Limited | Administrative & support | 2015 |
| Securit Group Limited | Administrative & support | 2015 |
| Securit(East Midlands) Ltd | Administrative & support | 2012 |
| Security Staffing and Events Ltd | Administrative & support | 2017 |
| Seen on Tiktok Limited | Wholesale & retail | 2024 |
| Semlocal Limited | Wholesale & retail | 2020 |
| Sends Support C.I.C. | Education | 2018 |
| Sentinel Sales & Marketing Limited | Administrative & support | 2005 |
| Sentor Services Limited | Hospitality | 2017 |
| Sequa Limited | Extraterritorial | 1994 |
| Serecena Fourteen Limited | Administrative & support | 2005 |
| SF Pharma Consulting Ltd | Professional & scientific | 2019 |
| Shadows of a Silhouette Ltd | Information & communication | 2025 |
| Shagana's Ltd | Wholesale & retail | 2022 |
| Shane Kinnear Property Limited | Real estate | 2022 |
| Shaun Matthews Joinery Ltd | Construction | 2022 |
| Shaw Snacks Ltd | Hospitality | 2024 |
| Shaw Wood Farm Holiday Lets Limited | Hospitality | 2019 |
| Shaznabi Limited | Hospitality | 2025 |
| Sheffield Trauma Centre Ltd | Health & social care | 2020 |
| Shepherd Hydraulics & Pneumatics Limited | Manufacturing | 1995 |
| Shermbuild Ltd | Construction | 2017 |
| Sherwood Forresters Longbow Archery Club | Arts & recreation | 2008 |
| Shine on Valeting Limited | Wholesale & retail | 2006 |
| Shirland Country Club Ltd | Arts & recreation | 2020 |
| Shirland Golf Club Members | Arts & recreation | 2011 |
| Shirley HR Limited | Administrative & support | 2013 |
| Shiva G Ltd | Hospitality | 2025 |
| Shopifit Ltd | Wholesale & retail | 2024 |
| Short ROW Holdings Limited | Financial services | 2024 |
| Shri Supermarket Limited | Wholesale & retail | 2012 |
| SID Hunt Golf Consultancy Ltd | Education | 2026 |
| Signal Fire Limited | Other services | 2011 |
| Signal Fire Protection Limited | Professional & scientific | 2001 |
| Signal House Group Limited | Professional & scientific | 2003 |
| Signal House Limited | Manufacturing | 1988 |
| Signature Hair Alfreton Ltd | Other services | 2023 |
| Silomarg Ltd | Administrative & support | 2014 |
| Silverhill Farm Ltd | Agriculture & fishing | 2023 |
| Simbulis Ltd | Arts & recreation | 2025 |
| Simon Carlin Limited | Construction | 2004 |
| Simona SY Ltd | Professional & scientific | 2021 |