| 121 Transportation Ltd | Transport & logistics | 2025 |
| 1440 Holdings Limited | Financial services | 2020 |
| 15 & 17 Peach Street Limited | Households | 2018 |
| 26 Ltd | Information & communication | 1998 |
| 2K Polymer Systems Limited | Manufacturing | 2007 |
| 3J Consulting Limited | Professional & scientific | 2024 |
| 4 Life Education Ltd | Education | 2025 |
| 47 Lettings Ltd | Real estate | 2020 |
| 4LIFE Explorers Ltd | Education | 2026 |
| 67 Hedgerows Ltd | Professional & scientific | 2025 |
| 808 Create Limited | Arts & recreation | 2013 |
| 80S Video Shop Ltd | Administrative & support | 2024 |
| 8XDOOR Ltd | Manufacturing | 2022 |
| 90 Degrees Coffee FIX Ltd | Manufacturing | 2025 |
| 97 Social Ltd | Professional & scientific | 2023 |
| A & a Motors Limited | Wholesale & retail | 2005 |
| A & P Properties (Coventry) Ltd | Real estate | 2022 |
| A D Entertainment Ltd | Hospitality | 2024 |
| A H Peabody Consulting Limited | Construction | 2022 |
| A H&F Power Services Ltd | Construction | 2025 |
| A L Vehicle Transport Ltd | Transport & logistics | 2020 |
| A M Holiday Cottages Ltd | Hospitality | 2024 |
| A Wardley and SON Ltd | Construction | 2022 |
| A&B Fabrication & Welding Contracting Services Ltd | Manufacturing | 2020 |
| A-Chem Holdings Limited | Financial services | 2026 |
| A-Chem Plant & Equipment Limited | Administrative & support | 2008 |
| A.E.C. Airconditioning and Refrigeration Services Ltd | Construction | 2014 |
| A.S.S.J Limited | Wholesale & retail | 2017 |
| A38 Car Storage Limited | Transport & logistics | 2016 |
| AA Convenience Store Ltd | Wholesale & retail | 2026 |
| Aaavixels Ltd | Wholesale & retail | 2026 |
| Aarcher Limited | Wholesale & retail | 2017 |
| AB Law Limited | Financial services | 2011 |
| ABB Events Ltd | Other services | 2021 |
| Abbey Cookers Ltd | Wholesale & retail | 2023 |
| ABC Mobility Group Ltd | Manufacturing | 2020 |
| ABC Mobility Holdings Ltd | Manufacturing | 2016 |
| ABC Mobility Property Ltd | Real estate | 2020 |
| Abds (Midlands) Ltd | Professional & scientific | 2012 |
| Abstracked Developments Limited | Other services | 2020 |
| Abstracked Solutions Limited | Professional & scientific | 2007 |
| Abveko Limited | Hospitality | 2018 |
| Acanthus Cast Stone Limited | Manufacturing | 2006 |
| Accolade Support and Care Ltd | Health & social care | 2013 |
| Accountancy AD Ltd | Professional & scientific | 2019 |
| Ace Pest Control Services Ltd | Other services | 2019 |
| ACG Projects Ltd | Real estate | 2023 |
| Acorn Exclusive Buildings and Developments Ltd | Construction | 2020 |
| Acorn Exclusive Buildings Limited | Construction | 2026 |
| ACP Homecare Ltd | Health & social care | 2016 |
| Acute Brickwork Ltd | Construction | 2020 |
| AD Miniprints Limited | Manufacturing | 2026 |
| Adevent (Midlands) Limited | Administrative & support | 2011 |
| Adex Services Limited | Administrative & support | 2001 |
| Adore Collections Ltd | Professional & scientific | 2012 |
| Adrob Services Ltd | Transport & logistics | 2019 |
| ADS Testing Ltd | Professional & scientific | 2018 |
| Advanced Mortgage Solutions Derbyshire Ltd | Financial services | 2016 |
| Advanced Performance Engineering Ltd | Administrative & support | 2021 |
| Advanced Transport Energy Solutions Limited | Professional & scientific | 2023 |
| Adventure Bikes Ltd | Education | 2022 |
| AEH Alfreton Ltd | Financial services | 2025 |
| Afish Holdings Ltd | Wholesale & retail | 2017 |
| Afrobrits-Scran Ltd | Hospitality | 2025 |
| Agritastoys Ltd | Manufacturing | 2025 |
| AHM Marketing Ltd | Professional & scientific | 2023 |
| Air Compressor Maintenance Ltd | Manufacturing | 2021 |
| AJ Turner Limited | Financial services | 2011 |
| AJM Group Limited | Real estate | 2015 |
| Ajpk Properties Ltd | Real estate | 2025 |
| AJS Swanwick Ltd | Hospitality | 2023 |
| Ajsrgs Ltd | Wholesale & retail | 2025 |
| AK Installations (Alfreton) Ltd | Construction | 2017 |
| AK Upholstery Ltd | Manufacturing | 2023 |
| AKH Systems Ltd | Construction | 2025 |
| Akorn Solutions Limited | Professional & scientific | 2020 |
| Akramatic Engineering Co. Holdings Limited | Financial services | 2025 |
| Akramatic Engineering Co. Limited | Manufacturing | 1967 |
| Akse Chemicals Limited | Wholesale & retail | 2013 |
| Alcazaba Group Limited | Professional & scientific | 2015 |
| Alchemy Lettings Limited | Real estate | 2024 |
| Alchemy Property and Investments Limited | Real estate | 2024 |
| ALD Transport Consultants Limited | Transport & logistics | 2024 |
| Alfrecell Holdings Limited | Professional & scientific | 2023 |
| Alfrecell Limited | Manufacturing | 1960 |
| Alfreton Accountants Limited | Professional & scientific | 2014 |
| Alfreton Autos Ltd | Wholesale & retail | 2021 |
| Alfreton Barbers Limited | Other services | 2024 |
| Alfreton Electrics Limited | Construction | 2013 |
| Alfreton Insurance Services (Holdings) Limited | Professional & scientific | 2014 |
| Alfreton Insurance Services Limited | Financial services | 1988 |
| Alfreton Investment Limited | Real estate | 2026 |
| Alfreton Metro Bathrooms Limited | Wholesale & retail | 2015 |
| Alfreton Mumbai Masala Limited | Hospitality | 2022 |
| Alfreton Music Tuition Limited | Arts & recreation | 2025 |
| Alfreton Properties (JJ) Limited | Real estate | 2001 |
| Alfreton Properties JJ Holdings Ltd | Households | 2023 |
| ALL About Bricks Limited | Wholesale & retail | 1991 |
| ALL SOX Limited | Manufacturing | 1993 |
| Allen (Derbyshire) Ltd | Administrative & support | 2014 |