| Alliance Property Services (Derbyshire) Limited | Construction | 2025 |
| Allied Brand Packaging Limited | Manufacturing | 2017 |
| Alloga UK Limited | Wholesale & retail | 1996 |
| Allroads Training Ltd | Education | 2013 |
| Allsop and SON Ltd | Manufacturing | 2014 |
| Alpha Pump Limited | Construction | 2018 |
| Alter EGO Brewing Co Ltd | Manufacturing | 2018 |
| Altriva 360 Advisory Limited | Professional & scientific | 2026 |
| Alunet Systems Limited | Professional & scientific | 2016 |
| Aman Lounge Ltd | Hospitality | 2023 |
| Amber Autos (Alfreton) Ltd | Wholesale & retail | 2019 |
| Amber Barn Assets Ltd | Administrative & support | 1991 |
| Amber Langis Limited | Administrative & support | 2008 |
| Amber Taxis (Alfreton) Limited | Transport & logistics | 1993 |
| Amber Valley Driveways Ltd | Construction | 2013 |
| Amber Valley Locksmiths Ltd | Other services | 2024 |
| Amber Valley Podiatry Ltd | Health & social care | 2026 |
| Amber Valley Practice Limited | Health & social care | 2022 |
| Amber Valley Scaffolding Limited | Construction | 2002 |
| Amber Valley Stone Limited | Manufacturing | 2019 |
| Amber Valley Training Ltd | Arts & recreation | 2009 |
| Amber Valley Wines Ltd | Manufacturing | 2011 |
| AMD Construct Ltd | Construction | 2018 |
| American Nails North 2 Limited | Other services | 2013 |
| AMH Services Ltd | Transport & logistics | 2026 |
| Ammachi Ltd | Wholesale & retail | 2025 |
| Ampco 113 Limited | Extraterritorial | 2015 |
| Amplify Audio Ltd | Information & communication | 2013 |
| AN Extra Hand Ltd | Construction | 2020 |
| Andrews the Builders Ltd | Construction | 2014 |
| Andy Stevens Plumbing Ltd | Construction | 2013 |
| Anlp International CIC | Education | 2005 |
| ANN Finance Ltd | Financial services | 2013 |
| Anna Pumer Photography Ltd. | Professional & scientific | 2012 |
| Annesley Park Limited | Real estate | 2015 |
| Annsco Care Solutions Ltd | Professional & scientific | 2018 |
| AP Flooring Specialists Ltd | Construction | 2021 |
| AP Installations (Alfreton) Ltd | Construction | 2023 |
| Apex Building Contractors Midlands Ltd | Construction | 2025 |
| Apexez Limited | Wholesale & retail | 2020 |
| Appliance Team (Midlands) Ltd | Wholesale & retail | 2013 |
| Aquacover Limited | Manufacturing | 2002 |
| AR Bridges Property Ltd | Real estate | 2026 |
| AR Recycling (Somercotes) Ltd | Water & waste | 2022 |
| Archie’S HOP in Bar Ltd | Hospitality | 2025 |
| Architectural Building Design Services Ltd | Professional & scientific | 2020 |
| Ariane Medical Systems Limited | Manufacturing | 2004 |
| Arise Christian Tutor Centre Ltd | Education | 2022 |
| ARK Landscapes Ltd | Construction | 2026 |
| ARK Training and Recruitment Limited | Administrative & support | 2024 |
| Arpyl Ltd | Professional & scientific | 2023 |
| Arro GOC Ltd | Wholesale & retail | 2012 |
| Arrow Homes (East Midlands) Limited | Hospitality | 2000 |
| Art of Metal Ltd | Manufacturing | 2016 |
| Artemis Asset Finance Limited | Financial services | 2026 |
| Artisan Home & Garden Ltd | Other services | 2017 |
| Artliff Design and Print Limited | Professional & scientific | 2019 |
| Aruba Island Stables Limited | Agriculture & fishing | 2020 |
| Asak Limited | Wholesale & retail | 2025 |
| Ascend Mountaineering Ltd | Arts & recreation | 2023 |
| Ashdale Flooring Limited | Construction | 2018 |
| Asher Lane Service Centre Limited | Wholesale & retail | 2010 |
| Ashfield Access Ltd | Construction | 2023 |
| Ashfield Nominees (2000) Limited | Extraterritorial | 2000 |
| Ashley Mark Catering Ltd | Hospitality | 2023 |
| Ashmere Derbyshire Limited | Health & social care | 2016 |
| Askmark Ltd | Professional & scientific | 2024 |
| ASL Pubs Ltd | Hospitality | 2023 |
| Aspyre Growth Ltd | Construction | 2016 |
| Asset Finance Compared Limited | Administrative & support | 2016 |
| Aston Roofing Services Ltd | Construction | 2021 |
| Astro & Dill Limited | Wholesale & retail | 2024 |
| AT Creative Ltd | Information & communication | 2011 |
| ATE Electrical Ltd | Extraterritorial | 2024 |
| Atlas Leather Limited | Manufacturing | 2025 |
| Attollo Consultants Ltd | Real estate | 2025 |
| Aurora Engineering Midlands Ltd | Professional & scientific | 2022 |
| Autoburkon Limited | Water & waste | 2025 |
| Autochair Limited | Manufacturing | 1990 |
| Autograph World Ltd | Wholesale & retail | 2004 |
| Autoreglaze Ltd | Wholesale & retail | 2018 |
| Autumn Group Limited | Professional & scientific | 2022 |
| Autumn Topco Limited | Professional & scientific | 2022 |
| Available Castles Ltd | Administrative & support | 2026 |
| B Insured Ltd | Financial services | 2005 |
| B.P. Caravan Storage Ltd | Transport & logistics | 2018 |
| Baldwin & Wiser Limited | Manufacturing | 1974 |
| Baler Options Ltd | Wholesale & retail | 2007 |
| Baltass Ltd | Wholesale & retail | 2026 |
| Banbury Bricks Limited | Extraterritorial | 2007 |
| Barat Alte UK Limited | Manufacturing | 2024 |
| Barlow Properties(Midlands) Limited | Real estate | 2021 |
| Bartram's Events Derbyshire Limited | Arts & recreation | 2024 |
| Bartram's Events LLP | | 2013 |
| Barudan UK Limited | Administrative & support | 2000 |
| BB Lighting Limited | Manufacturing | 2014 |
| BB Reformer Studio Ltd | Arts & recreation | 2026 |
| BE Optimised Ltd | Information & communication | 2021 |
| Beach's Bakery Limited | Manufacturing | 2020 |
| Beats ALL Transport Limited | Transport & logistics | 2020 |