| Sparkling Clean York Ltd | Administrative & support | 2025 |
| Sparkwright Electrics Ltd | Other services | 2017 |
| Sparro Limited | Information & communication | 2021 |
| Spartan Associates Ltd | Professional & scientific | 2020 |
| Spatial Projects Ltd | Professional & scientific | 2026 |
| Spawnpoiint Ltd | Information & communication | 2021 |
| SPB Contracting Ltd | Professional & scientific | 2024 |
| Speak Carbon Collective CIC | Education | 2021 |
| Speartooth Studios Ltd | Information & communication | 2019 |
| Special Director Services Limited | Professional & scientific | 2006 |
| Special Friends Animal Medical Centre Limited | Professional & scientific | 2005 |
| Special Voli Catering Ltd | Manufacturing | 2024 |
| Specialised Drone Services Limited | Agriculture & fishing | 2024 |
| Specialist Cars Transport Services Limited | Transport & logistics | 2003 |
| Specialist Construction Supplies Limited | Construction | 1989 |
| Specialist Courseware Limited | Information & communication | 2022 |
| Specialist Drinks Ltd | Wholesale & retail | 2020 |
| Specialist Healthcare Property Services Ltd | Real estate | 2016 |
| Specialist Maritime Ltd | Transport & logistics | 2016 |
| Specialist Property Advisers UK LLP | | 2010 |
| Speckled Wood (Carlisle) Management Company Limited | Households | 2016 |
| Spectacle Planning Limited | Information & communication | 2008 |
| Spectaculars International Limited | Arts & recreation | 2023 |
| Spectre Capital Consultancy Ltd | Professional & scientific | 2026 |
| Spectrum Consultancy (UK) Limited | Professional & scientific | 2014 |
| Spectrum First Education Limited | Other services | 2019 |
| Spectrum First Limited | Other services | 2020 |
| Spectrum First Professional Limited | Other services | 2022 |
| Speedbird Properties Ltd | Real estate | 2016 |
| Speedkix Ltd | Arts & recreation | 2018 |
| Speedquizzing Limited | Information & communication | 2011 |
| Speedy Street Solutions Ltd | Manufacturing | 2018 |
| Speight Engineering Ltd | Manufacturing | 2010 |
| Spellzone Limited | Education | 2009 |
| Spen View (Congleton) Management Company Limited | Real estate | 2018 |
| Spence Lettings Limited | Real estate | 2023 |
| Spencer Elliott Ltd | Professional & scientific | 2021 |
| Spencer Elliott Property Lawyers LLP | | 2016 |
| Spencer Woods (North East) Limited | Professional & scientific | 2016 |
| Spencer Woods (York) Limited | Professional & scientific | 2015 |
| Spencer Woods Limited | Professional & scientific | 2011 |
| Spero Property Developments Limited | Real estate | 2016 |
| Speros Solutions Limited | Real estate | 2019 |
| SPG Haulage and Logistics Support Ltd | Transport & logistics | 2026 |
| SPG HGV Ltd | Transport & logistics | 2026 |
| SPG Land Assets Ltd | Real estate | 2026 |
| SPG UK Group Ltd | Real estate | 2023 |
| Sphere-Alfa Limited | Mining & quarrying | 2004 |
| Spice 21 Ltd | Hospitality | 2023 |
| Spice 4 U Helmsley Ltd | Hospitality | 2025 |
| Spice Hub Copmanthorpe Ltd | Hospitality | 2025 |
| Spicebox York Ltd | Wholesale & retail | 2026 |
| Spicevillage Haxby Limited | Hospitality | 2021 |
| Spink Farming Ltd | Agriculture & fishing | 2013 |
| Spinners Brook Management Company Limited | Households | 2015 |
| Spinning a Yarn Ltd | Administrative & support | 2018 |
| Spira Management Limited | Professional & scientific | 2006 |
| Spirit of Japan Limited | Administrative & support | 2020 |
| Splash Pools Limited | Manufacturing | 2018 |
| Splash Swim Academy Ltd | Education | 2011 |
| Splash Swim LLP | | 2023 |
| Splashing COW Limited | Wholesale & retail | 2024 |
| SPM Production Engineering Limited | Professional & scientific | 2017 |
| Spns Investments Limited | Real estate | 2018 |
| Spoonfish Ltd | Information & communication | 2025 |
| Sport Acuity Limited | Professional & scientific | 2010 |
| Sport and Beyond Limited | Education | 2015 |
| Sporting Connexions Limited | Arts & recreation | 2008 |
| Sports Media Consulting Limited | Professional & scientific | 2008 |
| Sports Punk Limited | Wholesale & retail | 2014 |
| Sports Studio UK Ltd | Information & communication | 2024 |
| Sports Turf Services (York) Ltd | Construction | 2010 |
| Sportsbroker Holdings Limited | Financial services | 2022 |
| Sportselect Limited | Agriculture & fishing | 1977 |
| Spotlight Dance Academy Ltd | Arts & recreation | 2023 |
| Spotlight Investments Ltd | Households | 2023 |
| SPP Film Ltd | Information & communication | 2022 |
| Spray-Trac Systems Limited | Manufacturing | 1994 |
| Spraykraft Limited | Wholesale & retail | 2005 |
| Sprayplast UK Limited | Construction | 2023 |
| Spring Chicken Catering Ltd | Hospitality | 2023 |
| Spring Childcare Agency Ltd. | Administrative & support | 2023 |
| Spring Fields Advisors Limited | Professional & scientific | 2024 |
| Spring House Farm Estate Limited | Other services | 2018 |
| Spring House Investments Limited | Real estate | 2024 |
| Springbank Health Limited | Health & social care | 2013 |
| Springboard Collective Limited | Professional & scientific | 2025 |
| Springfield Close Management Company Ltd | Households | 2016 |
| Springfield Properties NE LLP | | 2020 |
| Springfir Estates Limited | Construction | 1969 |
| Springfir Holdings Limited | Extraterritorial | 1971 |
| Springs Estates (Lincolnshire) Ltd | Other services | 1974 |
| Springs Finance Limited | Financial services | 2014 |
| Spruce & Hawe Limited | Agriculture & fishing | 2005 |
| Spruce & Hawe Properties Limited | Real estate | 2014 |
| Spycatcher Limited | Manufacturing | 2000 |
| SQL Datascout Limited | Information & communication | 2010 |
| Sqlwatch Ltd | Information & communication | 2024 |
| Square Deals Limited | Construction | 2004 |
| Square PI Limited | Information & communication | 2002 |