| Drumrauch Hall Management Company Limited | Households | 2001 |
| DSN Connect Ltd | Wholesale & retail | 2025 |
| DSW Building Ltd | Construction | 2017 |
| DSW Renovations Ltd | Construction | 2020 |
| DT Barbering Ltd | Other services | 2018 |
| Dynamite Entertainment Ltd | Arts & recreation | 2019 |
| Eamc Ltd | Information & communication | 2019 |
| Ebor Agronomy Limited | Wholesale & retail | 2009 |
| Edmond Manor Ltd | Financial services | 2026 |
| Edwards Electrical Solutions Ltd | Administrative & support | 2015 |
| Ee&R Ltd | Construction | 2019 |
| Eksakt AEC Ltd | Professional & scientific | 2024 |
| Elder Lester Limited | Professional & scientific | 2003 |
| Electrical Technician Services Limited | Construction | 2008 |
| Elevate Hair Enhancers Ltd | Other services | 2025 |
| Elevate NE Limited | Professional & scientific | 2021 |
| Elif 2 Ltd | Hospitality | 2025 |
| Elizabeth Rose Aesthetics Ltd | Other services | 2021 |
| ELJ Metcalfe Ltd | Real estate | 2025 |
| Ellesfield Investments Limited | Real estate | 2021 |
| Ellesfield Limited | Manufacturing | 2014 |
| Eltech Services Ltd | Professional & scientific | 2024 |
| Elvet Structures Limited | Manufacturing | 1986 |
| Emanuel Spence Maintenance Services Limited | Construction | 2009 |
| Emkas Engineering Ltd | Professional & scientific | 2024 |
| Emral Limited | Professional & scientific | 2000 |
| Enable Civil Engineering Ltd | Construction | 2015 |
| Endurotrek Ltd | Wholesale & retail | 2024 |
| Engicad Limited | Professional & scientific | 2002 |
| Engineering Fusion Ltd | Information & communication | 2026 |
| Engineering Recruitment & Services Ltd | Administrative & support | 2018 |
| Enhance Dental Care Limited | Health & social care | 2007 |
| Enterpen (Hutton Rudby) Management Company Limited | Households | 2001 |
| Equitvain Limited | Real estate | 1985 |
| ESA Consult Ltd | Professional & scientific | 2021 |
| ESA Europe Ltd | Real estate | 2016 |
| Essandarr Ltd | Other services | 2003 |
| Events Durham and Teesside Limited | Other services | 2012 |
| Evolve Children Limited | Arts & recreation | 2016 |
| Evolve Fitness Yarm Ltd | Arts & recreation | 2025 |
| Excelera Limited | Professional & scientific | 2009 |
| Exchange Tech Ltd | Wholesale & retail | 2017 |
| Executive Promise Ltd | Hospitality | 2024 |
| Exited Blockchain Industries Ltd | Information & communication | 2023 |
| Fabricore Limited | Construction | 2012 |
| Famous Flyers Ltd. | Transport & logistics | 2023 |
| Farming Footprints Limited | Administrative & support | 2008 |
| Feyzoglu (NE) Limited | Other services | 2025 |
| Finch Event Group Ltd | Information & communication | 2019 |
| Finding ME Play Therapy Ltd | Education | 2025 |
| Fine & Performance Chemicals Limited | Manufacturing | 2009 |
| Fitzadlem DE Burgo Limited | Arts & recreation | 2011 |
| Flaming Farmers Ltd | Wholesale & retail | 2023 |
| Foden Construction Ltd | Professional & scientific | 2004 |
| Foden Spence Construction Limited | Construction | 1985 |
| Forest Engineering Solutions Ltd | Construction | 2025 |
| Forest Lane Properties Limited | Real estate | 2024 |
| Fort Lettings Limited | Real estate | 2019 |
| FOX Covert Inn Ltd | Hospitality | 2018 |
| FRAMEWORK7 Ltd | Information & communication | 2026 |
| Frazer Summers Consultancy Ltd | Professional & scientific | 2025 |
| Freeman Jewellers Ltd | Wholesale & retail | 2001 |
| FTF Property Ltd | Real estate | 2019 |
| Fulmine Mercantile Company Limited | Wholesale & retail | 2022 |
| Function 2.0 Ltd | Arts & recreation | 2025 |
| Function 3.0 Ltd | Other services | 2026 |
| Function 4.0 Ltd | Other services | 2026 |
| Function Hub Teesside Ltd | Other services | 2025 |
| Futurum (19) Limited | Energy | 2012 |
| G Bailey Mechanical Services Ltd | Manufacturing | 2026 |
| Gallagher Advisory Ltd | Financial services | 2024 |
| GEM Heating and Electrical Ltd | Construction | 2024 |
| GEROS2 Limited | Health & social care | 2017 |
| Gillian Griffiths Limited | Real estate | 1985 |
| Ginger Investment Holdings Ltd | Financial services | 2021 |
| GJ Groundworks Limited | Construction | 2020 |
| Glass Tint Limited | Professional & scientific | 2009 |
| GLD Solutions Limited | Information & communication | 2015 |
| Global Advantage International Limited | Wholesale & retail | 2016 |
| Global Inspection Services Ltd | Professional & scientific | 2025 |
| GLS Driver Training Limited | Education | 2020 |
| Gm&I Holings Ltd | Construction | 2022 |
| Gowland White (Surveyors & Estate Agents) Limited | Real estate | 1988 |
| GP Rigging and Rail Services Ltd | Manufacturing | 2019 |
| Graham Sorrell Limited | Extraterritorial | 2008 |
| Grammatology Group Limited | Professional & scientific | 2024 |
| Grant`S Technology & Engineering Limited | Professional & scientific | 2008 |
| Green Bricks Building Solutions Ltd | Construction | 2017 |
| Green Bricks Property Solutions Ltd | Households | 2020 |
| Green Vets Limited | Professional & scientific | 2023 |
| Greenacres Architecture Ltd | Professional & scientific | 2023 |
| Gregerson Estates Ltd | Real estate | 2018 |
| GS Doble Enterprise Limited | Construction | 2020 |
| GTG Financial Planning Ltd | Financial services | 2013 |
| Guardian Angels Counselling Ltd | Health & social care | 2020 |
| Guardian Gates Limited | Professional & scientific | 2022 |
| Gunn Services Ltd | Professional & scientific | 2022 |
| H Teasdale Consultancy Limited | Education | 2018 |
| H&H Housing Ltd | Households | 2021 |
| H. & J. Forbes Limited | Manufacturing | 1986 |