| DR H Downward Limited | Health & social care | 2023 |
| DR H Khan Limited | Health & social care | 2015 |
| DR K Manoj Limited | Health & social care | 2022 |
| DR K'S Clinic Ltd | Health & social care | 2018 |
| DR Laura Aesthetics Ltd | Health & social care | 2024 |
| DR NM Leaver Ltd | Health & social care | 2012 |
| DR Rachelle Walker Aesthetics Ltd | Health & social care | 2025 |
| Dragon Beauty Limited | Hospitality | 2014 |
| Dragon Blood Diagnostics Ltd | Health & social care | 2026 |
| Dragon Cars Ltd | Transport & logistics | 2008 |
| Dragon City Cleaning Ltd | Administrative & support | 2018 |
| Dragon City RL Limited | Hospitality | 2025 |
| Dragon Desserts Limited | Hospitality | 2025 |
| Dragon Field Sports Limited | Wholesale & retail | 2003 |
| Dragon Hydraulics (North Wales) Limited | Manufacturing | 2004 |
| Dragon Pharma Consultancy Ltd | Professional & scientific | 2017 |
| Dragon Trak Ltd | Extraterritorial | 2025 |
| Dragonflight Drones Ltd | Information & communication | 2020 |
| Dragons Nest Gaming Ltd | Arts & recreation | 2021 |
| Draig Supported Living Limited | Education | 2021 |
| Draycott Automotive Limited | Wholesale & retail | 2019 |
| Drayk Limited | Professional & scientific | 2022 |
| Dream and DO Limited | Financial services | 2025 |
| Dream Contracting Limited | Construction | 2024 |
| DRH Fabrications Ltd | Manufacturing | 2021 |
| Driven by Q Ltd | Transport & logistics | 2007 |
| Driven Plus Ltd | Information & communication | 2023 |
| Driving Adviser Ltd | Information & communication | 2018 |
| DRJ Projects Ltd | Construction | 2023 |
| DRP Estates Ltd | Construction | 2017 |
| DRP Joinery Ltd | Construction | 2007 |
| DRT Electrical Contracting Ltd | Construction | 2025 |
| Drumreed Limited | Construction | 1981 |
| Dryaras Ltd | Health & social care | 2023 |
| DS & DK Properties Ltd | Real estate | 2020 |
| DS Electrical (North West) Limited | Construction | 2026 |
| DS Promotional Space Ltd | Professional & scientific | 2023 |
| DS Property Rentals Ltd | Real estate | 2019 |
| DS Steel Services Ltd | Manufacturing | 2021 |
| DSD (Agri) Limited | Professional & scientific | 2013 |
| DSG Ec&I Services Ltd | Professional & scientific | 2025 |
| DSG Joinery Contractors Ltd | Construction | 2015 |
| DT Furniture Ltd | Manufacturing | 2019 |
| DT Leisure Limited | Construction | 2019 |
| Duane Williams Productions Ltd | Information & communication | 2019 |
| Duckett Rentals Limited | Real estate | 2026 |
| Duct Hygiene Limited | Administrative & support | 2004 |
| Duffkirk Holdings Limited | Financial services | 2023 |
| Dulson Developments Limited | Construction | 2026 |
| Dunbrin Limited | Professional & scientific | 2020 |
| Duncan Jones Motor Services Ltd | Wholesale & retail | 2014 |
| Dunlugas Limited | Administrative & support | 2000 |
| Duttons Fine Foods Limited | Manufacturing | 2021 |
| Duty MET Limited | Arts & recreation | 2014 |
| Duynie Ingredients Limited | Manufacturing | 2007 |
| DW Care Solutions Ltd | Health & social care | 2019 |
| DW Electrical Ltd | Construction | 2026 |
| Dyfan Evans Industrial Design Ltd | Professional & scientific | 2023 |
| Dyfrdwy Solutions Limited | Transport & logistics | 2018 |
| Dynamic Centre for Children and Young People With Disabilities | Health & social care | 2003 |
| Dynamic Security Systems Ltd | Administrative & support | 2024 |
| DYS Franchise Consulting Ltd | Professional & scientific | 2022 |
| E & B Construction Limited | Construction | 2010 |
| E Imaging Limited | Health & social care | 2019 |
| E Jones Coaches Limited | Transport & logistics | 2025 |
| E&D Building and Maintenance (North West) Limited | Construction | 2025 |
| E&F Retrofit Services (NW) Ltd | Professional & scientific | 2024 |
| E. Cunnah & SON Limited | Wholesale & retail | 1978 |
| Eardley Property Co. Limited | Construction | 1982 |
| Earth Matters Building Limited | Construction | 2003 |
| Earwell Ltd | Health & social care | 2022 |
| Easows Spicespedia UK Limited | Wholesale & retail | 2024 |
| East Exped Ltd | Arts & recreation | 2022 |
| East Midlands Civils Limited | Construction | 2015 |
| Eastern Touch Takeaway Ltd | Hospitality | 2019 |
| Eastgate Commercial Finance Ltd | Financial services | 2020 |
| Eastwick Plant Centre Limited | Agriculture & fishing | 2013 |
| Eastwind Trade Consultant Ltd | Wholesale & retail | 2025 |
| Easy Home Repairs 247 Ltd | Financial services | 2022 |
| Eaton Business Services Ltd | Real estate | 2006 |
| Ebonie Unlimited Ltd | Administrative & support | 2020 |
| Ebonies Voice CIC | Education | 2025 |
| ECM Education Consultants Ltd | Professional & scientific | 2010 |
| Eco BEE Taxis Ltd | Transport & logistics | 2026 |
| Ecofied Limited | Extraterritorial | 2010 |
| Ecological Land Management Limited | Professional & scientific | 2006 |
| Ecology Consulting Ltd | Professional & scientific | 2022 |
| Ecommerce Digital Export Services Limited | Wholesale & retail | 2016 |
| Ecommerce Export (China) Limited | Professional & scientific | 2016 |
| Ecommerce Export Limited | Information & communication | 2017 |
| Ecommerce With Umair Ltd | Wholesale & retail | 2025 |
| Economic Intelligence Wales Limited | Professional & scientific | 2017 |
| Economic Wellbeing Group Limited | Professional & scientific | 2026 |
| Edcas Ltd | Extraterritorial | 2024 |
| Eden Construction (North West) Limited | Construction | 2012 |
| Eden Estates (North West) Limited | Construction | 2023 |
| Edenhouseuk Ltd | Hospitality | 2017 |
| Edgar DE Poray Visual Arts Ltd | Manufacturing | 2025 |
| Edge Scaffolding Services Ltd | Construction | 2003 |
| Ediarc Limited | Other services | 2024 |