| Quinn's Jewellery Ltd | Manufacturing | 2026 |
| R & J Homes (Gloucester) Limited | Construction | 2021 |
| R.T.F. Limited | Professional & scientific | 2001 |
| R.Wilkins Flooring Ltd | Construction | 2023 |
| R4JOB Group Ltd | Real estate | 2023 |
| Rago & DIX Quality Care Ltd | Health & social care | 2017 |
| Ramla Properties Limited | Real estate | 2025 |
| Raymond & Carol Consultancy Limited | Financial services | 2024 |
| Rcmg Services Ltd | Information & communication | 2024 |
| RDP Advertising & Marketing Limited | Professional & scientific | 2005 |
| Re:Nu Well-Being Ltd | Professional & scientific | 2017 |
| Reach R C Limited | Professional & scientific | 2008 |
| React Contracting Ltd | Information & communication | 2021 |
| Real Marketing Limited | Information & communication | 2016 |
| Real Retreats Limited | Other services | 2018 |
| Reconfort Ltd | Information & communication | 2020 |
| Red Security Ltd | Construction | 2024 |
| Reece Willmott Carpentry & Building Limited | Manufacturing | 2026 |
| Reefine Ltd | Other services | 2022 |
| Regenesis Management Ltd | Wholesale & retail | 2013 |
| REM49 Ltd | Water & waste | 2001 |
| Renishaw Medical AM Solutions Limited | Extraterritorial | 2017 |
| Renishaw Medical Limited | Extraterritorial | 2017 |
| Renishaw Neuro Solutions Ltd | Manufacturing | 2016 |
| Repsycho Ltd | Wholesale & retail | 2016 |
| Revtek Solutions Limited | Wholesale & retail | 2025 |
| Rhodes Effective Intelligence Trust | Education | 2000 |
| RIO Pools Limited | Extraterritorial | 2019 |
| Rise HR Ltd | Administrative & support | 2009 |
| Rite DOM Ltd | Real estate | 2019 |
| Riverpoint Bath Ltd | Real estate | 2009 |
| Robert Derrick Limited | Manufacturing | 1987 |
| Robin Reid (Reading) Limited | Administrative & support | 1995 |
| Roda Limited | Information & communication | 2022 |
| Roger Mcdermott Consulting Ltd | Professional & scientific | 2025 |
| ROM Partners Ltd | Hospitality | 2018 |
| Romany Engineering Services Ltd | Professional & scientific | 2014 |
| Romcro Partners Ltd | Hospitality | 2026 |
| Roneda Limited | Financial services | 2025 |
| Rooted Scaffolding Ltd | Construction | 2026 |
| Rosevelo.Cc Limited | Professional & scientific | 2016 |
| Rousham Medical Limited | Health & social care | 2016 |
| Rubiq Design Ltd | Professional & scientific | 2026 |
| RYE and Seaview Property Investments Ltd | Real estate | 2024 |
| S a Jacobs & Co Ltd | Professional & scientific | 2026 |
| S W Bennett Construction Ltd | Construction | 2021 |
| Safer Fostering CIC | Health & social care | 2020 |
| Sailing Cinema Ltd | Wholesale & retail | 2019 |
| SAS Consulting Services Ltd | Professional & scientific | 2025 |
| Saunders Roofing Group Ltd | Construction | 2024 |
| Scrl Ltd | Professional & scientific | 2025 |
| Sdjb Business Consultancy Ltd | Information & communication | 2018 |
| Secret Garden Cinema Ltd | Wholesale & retail | 2019 |
| Sennitt Limited | Real estate | 2019 |
| Shedlight Limited | Manufacturing | 2015 |
| Shield Healthcare Limited | Health & social care | 2015 |
| Shipton and Sons Construction Ltd | Construction | 2014 |
| Shirley Agricultural Contractors Limited | Agriculture & fishing | 2024 |
| Short Steps Ltd | Other services | 2018 |
| Shumbauk Ltd | Information & communication | 2016 |
| Silicon Edge Limited | Professional & scientific | 2014 |
| Simro Business Advisory Services Limited | Professional & scientific | 2023 |
| Simstec Ltd | Information & communication | 2004 |
| Sirius IP Limited | Professional & scientific | 2016 |
| Sixpence Core Ltd | Information & communication | 2025 |
| Snailshell Limited | Information & communication | 2006 |
| Sniper Limited | Information & communication | 2016 |
| Soils (HS) Ltd | Wholesale & retail | 1998 |
| Solum (SW) Ltd | Construction | 2006 |
| Solum SW Holdings Limited | Construction | 2022 |
| South West Construction Academy Limited | Education | 2013 |
| South West Contracting Ltd | Construction | 2025 |
| South West Maritime Academy Technical Services Ltd | Manufacturing | 2018 |
| SP Aviation Limited | Other services | 2018 |
| Speedway Flooring Limited | Construction | 2003 |
| Spheric (Plant Hire) Limited | Professional & scientific | 1976 |
| Sport Wotton Limited | Arts & recreation | 2014 |
| Spotless Domestic Cleaning Limited | Other services | 2018 |
| Sptf Limited | Professional & scientific | 2007 |
| Squirrel Solutions Ltd | Information & communication | 2021 |
| Srhnetworks Ltd | Information & communication | 2018 |
| SS4 Architects Ltd | Professional & scientific | 2012 |
| ST Albans Sporting Estates LLP | | 2021 |
| Stag and Lion Ltd | Hospitality | 2024 |
| Starling Kitchen Management Ltd | Professional & scientific | 2016 |
| Storkey Automotive Limited | Wholesale & retail | 2021 |
| Storkey Automotive Services Limited | Wholesale & retail | 2022 |
| Stormglass Publishing Limited | Information & communication | 2025 |
| Strategic Insurance Solutions Mena Limited | Financial services | 2011 |
| Strategic Land Projects Limited | Real estate | 2007 |
| Strategic Properties Limited | Real estate | 2015 |
| Strawberry Coaching Limited | Professional & scientific | 2007 |
| Studio Speed Ltd | Professional & scientific | 2026 |
| Stwr Catering Ltd | Hospitality | 2023 |
| Suki Engineering Limited | Professional & scientific | 2026 |
| Sunargha Ltd | Hospitality | 2025 |
| Sundayshill Limited | Professional & scientific | 2026 |
| Swan Hotel Market ST Limited | Hospitality | 2014 |
| T F Smith Limited | Information & communication | 2000 |
| T Greenstreet Building Ltd | Construction | 2021 |