| Supreme Shutters Sussex Ltd | Construction | 2025 |
| Suprendo Group Limited | Financial services | 2025 |
| Suprendo Technologies Limited | Information & communication | 2025 |
| Surefix Group 24/7 Ltd | Construction | 2025 |
| Surfbus UK Limited | Wholesale & retail | 2014 |
| Surge Solar Ltd | Construction | 2024 |
| Surgical Sense Ltd | Health & social care | 2016 |
| Surrey and Sussex Urology Services Ltd | Health & social care | 2016 |
| Surrey Dental Spa Limited | Health & social care | 2016 |
| Surrey Green Management Ltd | Construction | 2016 |
| Surrey Hills Families Eco Initiative CIC | Other services | 2022 |
| Surrey Lime and Building Limited | Construction | 2023 |
| Surrey M&E Limited | Construction | 2018 |
| Surrey Oaks Limited | Hospitality | 2014 |
| Surrey Road Haulage Limited | Extraterritorial | 2010 |
| Surya Medical Ltd | Health & social care | 2023 |
| Susie Holmes Consulting Limited | Administrative & support | 2010 |
| Sussex Accommodation Professionals Ltd | Real estate | 2022 |
| Sussex Advanced Glazing Limited | Construction | 2022 |
| Sussex Aerials and Satellites Limited | Information & communication | 2026 |
| Sussex Aluminium Systems Ltd | Manufacturing | 2016 |
| Sussex Auto Sales Limited | Wholesale & retail | 2018 |
| Sussex Bathroom and Kitchen Installations Ltd | Construction | 2018 |
| Sussex Bespoke Interiors Ltd | Construction | 2026 |
| Sussex Boat Fishing and Boat Deliveries Ltd | Agriculture & fishing | 2017 |
| Sussex Boiler Services Limited | Construction | 2019 |
| Sussex Build Ltd | Construction | 2025 |
| Sussex Building Specialists Ltd | Construction | 2024 |
| Sussex Building Surveyors Limited | Professional & scientific | 2010 |
| Sussex Bulk Limited | Transport & logistics | 2014 |
| Sussex Car Centre Limited | Wholesale & retail | 2016 |
| Sussex Carpentry Contractors Limited | Construction | 1994 |
| Sussex Catering Equipment (SCE) Ltd | Manufacturing | 2016 |
| Sussex Clinic Limited | Health & social care | 1995 |
| Sussex Coast Construction Ltd | Construction | 2020 |
| Sussex Coastal Property Ltd | Real estate | 2024 |
| Sussex Commercial Spares Ltd | Wholesale & retail | 2018 |
| Sussex Community Enterprises Ltd | Other services | 2015 |
| Sussex Construct Ltd | Construction | 2023 |
| Sussex County Construction Limited | Construction | 2017 |
| Sussex County Dog Training Limited | Other services | 2012 |
| Sussex County Driveways Limited | Construction | 2020 |
| Sussex County Investments Limited | Real estate | 2020 |
| Sussex County Windows Limited | Manufacturing | 2011 |
| Sussex Craftworks Ltd | Construction | 2025 |
| Sussex Creative Club Limited | Arts & recreation | 2025 |
| Sussex Data Ltd | Construction | 2020 |
| Sussex Design & Build Ltd | | 2012 |
| Sussex Design and Development - Bellacre, Storrington Ltd | Construction | 2018 |
| Sussex Design and Development Limited | Construction | 2015 |
| Sussex Electrical Contractors Ltd | Construction | 2019 |
| Sussex Estate Management Limited | Real estate | 2011 |
| Sussex Exterior Services Ltd | Construction | 2026 |
| Sussex F&B Trading (Sussex) Limited | Hospitality | 2026 |
| Sussex FAB Shop Ltd | Extraterritorial | 2016 |
| Sussex Floorings & Interiors Limited | Wholesale & retail | 2004 |
| Sussex Food Products Limited | Hospitality | 2025 |
| Sussex Football Hub Ltd | Information & communication | 2025 |
| Sussex Fuel Oils Limited | Wholesale & retail | 2017 |
| Sussex Function Caterers Ltd | Hospitality | 2017 |
| Sussex GT Vehicle Services Limited | Wholesale & retail | 2026 |
| Sussex Heritage Decorators Ltd | Construction | 2023 |
| Sussex Homes Limited | Real estate | 1988 |
| Sussex Host Ltd | Real estate | 2025 |
| Sussex Kitchen Studios Ltd | Wholesale & retail | 2024 |
| Sussex Knock Throughs Ltd | Construction | 2019 |
| Sussex Luxury Property Services Limited | Households | 2024 |
| Sussex Manures Limited | Manufacturing | 2023 |
| Sussex Markets Ltd | Wholesale & retail | 2018 |
| Sussex Meat Co. Ltd | Wholesale & retail | 2022 |
| Sussex Mechanical Engineering Services Ltd | Professional & scientific | 2011 |
| Sussex Medical Chambers Limited | Health & social care | 2004 |
| Sussex Moon Ltd | Financial services | 2021 |
| Sussex Motor Company Limited | Extraterritorial | 1987 |
| Sussex Optical Glazing Services Limited | Health & social care | 2010 |
| Sussex Oven Cleaning Limited | Administrative & support | 2014 |
| Sussex Palliative Orientated Research Centred Training and Education Ltd | Education | 2026 |
| Sussex Performance Centre Ltd | Arts & recreation | 2016 |
| Sussex Physio Pilates Limited | Health & social care | 2016 |
| Sussex Play Therapy Ltd | Health & social care | 2025 |
| Sussex Pond Doctor Ltd | Administrative & support | 2022 |
| Sussex Prestige Motors Ltd | Wholesale & retail | 2026 |
| Sussex Print Finishers Limited | Manufacturing | 1984 |
| Sussex Properties 2 U Ltd | Real estate | 2015 |
| Sussex Renewable Heating Ltd | Construction | 2023 |
| Sussex Renewable Services Ltd | Manufacturing | 2024 |
| Sussex Renovations Ltd | Construction | 2025 |
| Sussex Retirement Planning Limited | Financial services | 2020 |
| Sussex Road Haulage Limited | Extraterritorial | 2010 |
| Sussex Rolling Road (2001) Limited | Wholesale & retail | 2001 |
| Sussex Security Solutions Limited | Administrative & support | 2008 |
| Sussex Technologies Limited | Other services | 2026 |
| Sussex Therapy Ltd | Health & social care | 2021 |
| Sussex Tiling & Mastic Ltd | Construction | 2019 |
| Sussex Tyre Services Ltd | Wholesale & retail | 2025 |
| Sussex Vending Limited | Hospitality | 2014 |
| Sussex Violins Ltd | Manufacturing | 2023 |
| Sussex Vision Ltd | Health & social care | 2016 |
| Sussex Water Mains Limited | Water & waste | 2015 |
| Sussex Water Mains Services Limited | Water & waste | 2016 |