| Duffy Regan Ltd | Professional & scientific | 2010 |
| Duncan Fearnley Cricket Sales Limited | Manufacturing | 1975 |
| Duncan Willoughby Event Services Limited | Administrative & support | 2012 |
| Dunns Lane Management Company Limited | Hospitality | 1977 |
| Dunton House Management Limited | Extraterritorial | 1997 |
| Dunwell Electrical Limited | Construction | 2023 |
| Dustilie Day Nursery Ltd | Education | 2025 |
| Dusturz Ltd | Manufacturing | 2025 |
| Dusty Mouse Pubs Limited | Hospitality | 2016 |
| Duxfortis Limited | Hospitality | 2018 |
| DVS Heating & Plumbing Limited | Construction | 2020 |
| DW Systest Limited | Information & communication | 2012 |
| Dwhvac Ltd | Construction | 2022 |
| DX Commander Ltd | Professional & scientific | 2017 |
| Dymaxion Limited | Information & communication | 2018 |
| Dympsees Ltd | Information & communication | 2026 |
| Dynamechs Limited | Professional & scientific | 2009 |
| Dynamic Tension Recordings Limited | Arts & recreation | 2009 |
| Dynamiq Safety & Health Ltd | Professional & scientific | 2020 |
| Dynamometer Services Limited | Manufacturing | 1978 |
| E & E Hand Car Wash Ltd | Administrative & support | 2024 |
| E G Groundworks Ltd | Construction | 2021 |
| E U BIZ Ltd | Extraterritorial | 2003 |
| E U Properties Ltd | Extraterritorial | 2003 |
| E&A Care Ltd | Health & social care | 2018 |
| E&C Leisure Ltd | Hospitality | 2019 |
| E&G Holdings Ltd | Manufacturing | 2019 |
| E&S Property Solutions Limited | Real estate | 2020 |
| E&S Property Sourcing Limited | Real estate | 2020 |
| E-Bike City Racing Ltd | Arts & recreation | 2021 |
| E-Build Site Services Ltd | Construction | 2021 |
| E-Hops Engineering Ltd | Professional & scientific | 2025 |
| E-Retailmarkets Ltd. | Extraterritorial | 2000 |
| E-Tail 24/7 Limited | Wholesale & retail | 2015 |
| E-Web Corporation Limited | Extraterritorial | 2000 |
| E.A.M. Electrical and Mechanical Services Ltd | Manufacturing | 2011 |
| E.B Surgery Limited | Health & social care | 2019 |
| EAC Global Services Ltd | Hospitality | 2023 |
| Eagle Luxury Car Hire Ltd | Administrative & support | 2025 |
| Eagle Resources Ltd | Other services | 2019 |
| Earls Grange Management Company Limited | Households | 1995 |
| Earlywin Motors Limited | Wholesale & retail | 2024 |
| Earthen Tea Limited | Wholesale & retail | 2020 |
| Easier Ltd | Information & communication | 2010 |
| Easing Hill Vineyard LLP | | 2019 |
| Eastend Resources Ltd | Other services | 2023 |
| Eastmere Property Investments 2 Ltd | Real estate | 2026 |
| Eastmere Property Investments Ltd | Real estate | 2021 |
| EASY2SWITCH UK Limited | Information & communication | 2005 |
| Easywayforgoods Limited | Transport & logistics | 2024 |
| EAT Dome Ltd | Financial services | 2024 |
| EAT Greek Limited | Hospitality | 2020 |
| Eaton Timber Solutions Limited | Manufacturing | 1977 |
| Eazycapture Ltd | Information & communication | 2025 |
| Eazyrex Limited | Wholesale & retail | 2026 |
| EB Business Ltd | Administrative & support | 2024 |
| Ebowness Consulting Ltd | Agriculture & fishing | 2016 |
| Ecentury Limited | Information & communication | 2007 |
| Echo Business Solutions Limited | Financial services | 2014 |
| Eclat Property Limited | Real estate | 2020 |
| Eclectric Limited | Professional & scientific | 2017 |
| Eco Facilities Management Limited | Administrative & support | 2021 |
| Eco Stoves Limited | Extraterritorial | 2007 |
| ECO2PASSIVE Ltd | Construction | 2025 |
| Econ Express Ltd | Wholesale & retail | 2025 |
| Economaics Group Ltd | Information & communication | 2026 |
| Econova Waste Solutions Ltd | Water & waste | 2026 |
| Ecoteric Limited | Professional & scientific | 2007 |
| Ecrconsulting Ltd | Professional & scientific | 2025 |
| ED & Lou's Vintage World Limited | Other services | 2006 |
| Eden Eden Ltd | Other services | 2026 |
| Eden Group (Worcester) Limited | Construction | 2019 |
| Eden Property Assets Limited | Financial services | 2025 |
| Eden Rose Yoga Ltd | Other services | 2019 |
| Eden Wellbeing Limited | Other services | 2022 |
| Edge Adventure Limited | Wholesale & retail | 2025 |
| Edgeley Limited | Construction | 2001 |
| Edie Stays Ltd | Real estate | 2026 |
| Edit Global IP Limited | Wholesale & retail | 2026 |
| Edit Global Limited | Wholesale & retail | 2026 |
| Edit Ventures Limited | Financial services | 2026 |
| Education & Clinical Research & Innovation Group Limited | Professional & scientific | 2021 |
| Edugill Ltd | Education | 2025 |
| Eduprotech Ltd | Education | 2025 |
| Edvanced Ltd | Administrative & support | 2024 |
| Edwards Equine Ltd | Agriculture & fishing | 2022 |
| Edwards Locksmiths & Security Limited | Administrative & support | 2013 |
| Edwards Manufacturing Solutions Ltd | Professional & scientific | 2023 |
| Edwards of Worcester Ltd | Wholesale & retail | 2026 |
| Edwardson Loughlin Limited | Professional & scientific | 2020 |
| Eeden Group Limited | Health & social care | 2026 |
| Eempower CIC | Education | 2024 |
| EFL Health Ltd | Wholesale & retail | 2018 |
| Efre Limited | Real estate | 2026 |
| Egdon Group Limited | Extraterritorial | 2023 |
| Egdon Service Station Ltd | Wholesale & retail | 2002 |
| Eggcones Ltd | Administrative & support | 2019 |
| EHG Properties Ltd | Real estate | 2024 |
| EHL Marine Services Ltd | Professional & scientific | 2024 |
| Eighth House Limited | Information & communication | 2006 |