| Empowering U Healthcare Limited | Health & social care | 2002 |
| Empowering U Holdings Limited | Financial services | 2026 |
| Empowering U Limited | Hospitality | 2015 |
| Empress DA LUZ Ltd | Wholesale & retail | 2025 |
| Empressed C.I.C. | Information & communication | 2025 |
| EMR Enterprises Limited | Hospitality | 2023 |
| EMR Metal Recycling Ltd | Water & waste | 2023 |
| EN Enterprise Ltd | Information & communication | 2025 |
| Enable Carers Limited | Health & social care | 2020 |
| Enable-Ed CIC | Education | 2015 |
| Enabling Asset Management Services Limited | Real estate | 2016 |
| Enabling Consultancy Services Limited | Administrative & support | 2014 |
| Enchris Digital Media Ltd | Information & communication | 2026 |
| Encomore Ltd | Wholesale & retail | 2026 |
| Encore Networks Ltd | Wholesale & retail | 2024 |
| Endear Solutions Ltd | Administrative & support | 2024 |
| Endeavour Multi Academy Trust | Education | 2017 |
| Endies UK Limited | Transport & logistics | 2018 |
| Endurable Fasteners Limited | Manufacturing | 2025 |
| Endwood Court RTM Company Limited | Households | 2020 |
| Energize Electrical, Heating & Plumbing Services Limited | Construction | 2011 |
| Energizer Marketing Ltd | Professional & scientific | 2009 |
| Energy Angels Group Ltd | Administrative & support | 2016 |
| Energy Bolting Limited | Manufacturing | 2018 |
| Energy Works Plus Ltd | Construction | 2026 |
| Energyseal Windows & Doors Ltd | Construction | 2022 |
| Enerst Health Ltd | Health & social care | 2014 |
| Enerstogeorge Ltd | Professional & scientific | 2019 |
| Enforce Construction Services Ltd | Construction | 2025 |
| Engage Back Office Ltd | Administrative & support | 2023 |
| Engage First Page Ltd | Professional & scientific | 2021 |
| Engage Health Ltd | Health & social care | 2022 |
| Engineered Plastic Solutions Ltd | Wholesale & retail | 2016 |
| Engineering Real Results Limited | Education | 2012 |
| Engrave X Limited | Manufacturing | 2022 |
| Engserve (Mech) Limited | Professional & scientific | 2007 |
| Engwer Ltd | Transport & logistics | 2012 |
| Enhanced Fire & Security Solutions Ltd | Administrative & support | 2020 |
| Enich LOG Ltd | Transport & logistics | 2016 |
| Enisma Ltd | Construction | 2025 |
| Enjoyable C.I.C. | Health & social care | 2024 |
| Enliven Academy Ltd. | Wholesale & retail | 2020 |
| Enon Plus Limited | Hospitality | 2012 |
| Enpro Holdings Limited | Other services | 2019 |
| Enpro Maintenance Limited | Other services | 2023 |
| Enpro Services Limited | Other services | 2017 |
| Enpro Training Limited | Professional & scientific | 2023 |
| ENS FIX Ltd | Construction | 2026 |
| Entechpro Limited | Professional & scientific | 2023 |
| Enterprise Development & Education Network | Real estate | 2013 |
| Enterprise Homes Group Limited | Hospitality | 2014 |
| Enterprise MG Ltd | Professional & scientific | 2022 |
| Entire Facility Management Ltd | Administrative & support | 2024 |
| Entire Facility Services Ltd | Administrative & support | 2024 |
| Entra Freeholds Limited | Real estate | 2024 |
| Entra View Ltd | Real estate | 2024 |
| Entrance Hardware Limited | Wholesale & retail | 2024 |
| ENV Domestic Installation Services Limited | Other services | 2020 |
| Enville Properties Ltd | Real estate | 2014 |
| Enviro Sampling Solutions Ltd | Professional & scientific | 2023 |
| Envirogy Power Ltd | Administrative & support | 2018 |
| Environmental Solutions Through Partnership Limited | Professional & scientific | 2004 |
| Envirosafe Testing Limited | Professional & scientific | 2021 |
| Envisage Childrens Care Limited | Extraterritorial | 2020 |
| Envs Transport and Installation Limited | Transport & logistics | 2024 |
| Enzo Group Holdings Limited | Financial services | 2026 |
| Enzo Legal Services Ltd | Professional & scientific | 2023 |
| EP Heating Solutions Ltd | Construction | 2025 |
| Epac Holdings Europe Limited | Professional & scientific | 2019 |
| Epac UK Sheffield Limited | Manufacturing | 2021 |
| Epac UK Silverstone Limited | Manufacturing | 2019 |
| Epharma Limited | Health & social care | 2022 |
| Epie and Sons Limited | Health & social care | 2024 |
| Episode Limited | Real estate | 2020 |
| Eponawise Ltd | Administrative & support | 2017 |
| Equal Route Ltd | Professional & scientific | 2024 |
| Equestrian Property Ltd | Real estate | 2026 |
| Equinox Gifted Thoughts Limited | Wholesale & retail | 2000 |
| Equinox Property Management (Midlands) Limited | Households | 2025 |
| EQUIP2 Limited | Wholesale & retail | 2011 |
| Equity Access Limited | Professional & scientific | 2007 |
| Eric Construct Ltd | Construction | 2025 |
| Ericosen Ltd | Administrative & support | 2026 |
| Erika Auto Commerical Ltd | Wholesale & retail | 2022 |
| Erika Construction Ltd | Construction | 2026 |
| Erika Used Vehicle Ltd | Wholesale & retail | 2025 |
| Eriz Integrated Services Limited | Administrative & support | 2024 |
| Erminia Properties Limited | Real estate | 2025 |
| ERN Construction Limited | Construction | 2023 |
| Erneosa Limited | Wholesale & retail | 2017 |
| Ernie and Arthur Holdings Co Limited | Financial services | 2016 |
| Erno Kids Ltd | Extraterritorial | 2016 |
| Erol Naim Ltd | Transport & logistics | 2017 |
| ES Investments Ltd | Financial services | 2026 |
| ES Studios Ltd | Real estate | 2024 |
| Es&A Properties Limited | Real estate | 2008 |
| Esamni Company Limited | Health & social care | 2018 |
| Escapes and Estates Ltd | Real estate | 2022 |
| Esellers Emporium Ltd | Wholesale & retail | 2024 |
| Esenabla Real Estate Ltd | Real estate | 2019 |