| Duruna Limited | Construction | 2016 |
| Dutch Masters Limited | Wholesale & retail | 2006 |
| Dwellhouse Ltd | Manufacturing | 2024 |
| Dyad Clothing Ltd | Wholesale & retail | 2023 |
| Dybles Limited | Real estate | 2011 |
| Dylamic Limited | Wholesale & retail | 2023 |
| D’Oyleys Farm Limited | Wholesale & retail | 2021 |
| E P L S Design Limited | Administrative & support | 2002 |
| E Sproson Ltd | Health & social care | 2026 |
| E&J Ground Rents NO7 LP | | 2013 |
| E-Mobility Finance Limited | Financial services | 2022 |
| E-Pilot Ltd | Administrative & support | 2013 |
| E.D. and P.J. Whieldon Limited | Wholesale & retail | 2005 |
| E.J Hanson Group Ltd | Financial services | 2023 |
| Eagle Helm Consulting Ltd | Professional & scientific | 2023 |
| Eagle International (Europe) Limited | Professional & scientific | 2015 |
| Eagle International Capital Limited | Information & communication | 2022 |
| Eagle International Group Limited | Professional & scientific | 2015 |
| Eagle International Limited | Administrative & support | 2004 |
| Ealing Park Gardens Management Company Limited | Professional & scientific | 2005 |
| Eaph Holdings Ltd | Financial services | 2025 |
| Earth Below Films Limited | Information & communication | 2013 |
| Eastgate Court Residents Company Limited | Households | 1975 |
| Eastgate House (Freehold) Limited | Households | 2019 |
| Eastgate International Limited | Transport & logistics | 1988 |
| Eastgate Street Management Company Limited | Households | 2003 |
| Eastleigh Swim Club Limited | Arts & recreation | 2011 |
| Easton Garage Limited | Wholesale & retail | 2017 |
| Eaststern Limited | Financial services | 1982 |
| Easy BIN Ltd | Administrative & support | 2016 |
| Easytesters Limited | Professional & scientific | 2007 |
| Eats Trading Ltd | Hospitality | 2021 |
| EAZY2SHIP Benelux Ltd | Professional & scientific | 2025 |
| EB Airside Limited | Administrative & support | 2018 |
| EB Therapy Limited | Health & social care | 2013 |
| EBG Screening Limited | Financial services | 2016 |
| EC Hants EOT Ltd | Professional & scientific | 2024 |
| EC Winchester Ltd | Other services | 2019 |
| Ecco Marketing Limited | Professional & scientific | 2025 |
| Eclipse Car Sales Limited | Wholesale & retail | 2008 |
| Eclogite Limited | Information & communication | 2024 |
| Eco Interiors Winchester Limited | Wholesale & retail | 2017 |
| Eco Montessori Limited | Education | 2021 |
| Eco Washroom Supplies Ltd | Wholesale & retail | 2025 |
| Eco-Freight Limited | Transport & logistics | 2018 |
| Eco-Screen Limited | Professional & scientific | 2016 |
| ECO4UM Ltd | Information & communication | 2015 |
| Ecodale Limited | Information & communication | 2006 |
| Ecollective Travel Ltd | Professional & scientific | 2019 |
| Ecological Planning & Research Limited | Professional & scientific | 2013 |
| Ecosorba Ltd | Manufacturing | 2010 |
| Edan Haus Limited | Other services | 2025 |
| Edeb Limited | Professional & scientific | 2008 |
| Eden NMP Services Ltd | Health & social care | 2025 |
| Eden Property Letting Limited | Real estate | 1936 |
| Eden Summer Profits Ltd | Real estate | 2024 |
| Edge Demolition Ltd | Construction | 2019 |
| Edge Southern Contracts Limited | Construction | 2024 |
| Edge TS Developments Limited | Construction | 2023 |
| Editorial Office Limited | Information & communication | 2003 |
| Edney Trustees Limited | Extraterritorial | 2012 |
| EDW Data Limited | Information & communication | 2015 |
| Edwards Capital Ltd | Professional & scientific | 2025 |
| Efiwon Holding Limited | Financial services | 2021 |
| Egan & Bayliss Limited | Education | 2015 |
| Egbert Management Limited | Households | 1976 |
| Eggnest Childcare Ltd | Education | 2024 |
| EGH Lighting Limited | Manufacturing | 2008 |
| Eglantier Ltd | Wholesale & retail | 2011 |
| Ehtesham Properties Ltd | Real estate | 2017 |
| EHW Consultancy Limited | Professional & scientific | 2024 |
| Eighteen Wealth Planning Limited | Financial services | 2018 |
| Eighty Three Property Ltd | Real estate | 2026 |
| Eighty Two Construction Ltd | Construction | 2022 |
| EJ Warman Developments Ltd | Construction | 2020 |
| EJM Official Ltd | Wholesale & retail | 2025 |
| Elanse Cleaning Limited | Administrative & support | 2020 |
| Elatum Limited | Construction | 2016 |
| Elcress Properties & Furnishings Limited | Real estate | 2002 |
| Elecinno UK Ltd | Wholesale & retail | 2025 |
| Electric IT Ltd | Information & communication | 2019 |
| Electric Kids Limited | Administrative & support | 2024 |
| Electric Vehicle Association England CIC | Other services | 2020 |
| Elegance Bespoke Tailor Ltd | Manufacturing | 2024 |
| Elemental Automotive Consulting Ltd | Professional & scientific | 2020 |
| Elemental Automotive Group Limited | Manufacturing | 2012 |
| Elemental Cars Ltd | Extraterritorial | 2016 |
| Elemental Motor Company Limited | Manufacturing | 2012 |
| Elemental Racing Limited | Extraterritorial | 2012 |
| Elemental Wellness Hampshire Ltd | Health & social care | 2024 |
| Elementel (Installations & Maintenance) Ltd | Information & communication | 2013 |
| Elementel Ltd | Information & communication | 2012 |
| Elephant EAR Ltd | Manufacturing | 2023 |
| Elevate AI Tech Limited | Professional & scientific | 2025 |
| Elevate Software Solutions Limited | Construction | 2004 |
| Elevate SS Limited | Construction | 2019 |
| Elevate Training Group Ltd | Arts & recreation | 2023 |
| Elevatedelivery Limited | Administrative & support | 2024 |
| Elgin Court Management Limited | Households | 1996 |
| Elite FPS Limited | Construction | 2000 |