| Alex Dury & Co Limited | Construction | 2022 |
| Anything Direct Europe Limited | Wholesale & retail | 2002 |
| Apex Strategy Management Limited | Professional & scientific | 2022 |
| Art VS Business Limited | Information & communication | 2010 |
| ASB Kitchens Limited | Wholesale & retail | 2005 |
| B & S Felt Roofing Limited | Construction | 2015 |
| Beach Baby Ltd | Other services | 2025 |
| Bewell Athletic CIC | Education | 2024 |
| Break Point Coaching Ltd | Wholesale & retail | 2023 |
| Bruno & Maxwell Limited | Professional & scientific | 2010 |
| Cerebellum Group Ltd | Wholesale & retail | 2021 |
| Chardonnay RYE Limited | Hospitality | 2017 |
| Cherry House Properties Limited | Real estate | 2018 |
| Cinque Ports Sweep Limited | Other services | 2014 |
| Customs and Classics LLP | | 2016 |
| Devonshire Hotels (London) Limited | Hospitality | 2004 |
| E.C.T. (Conservation) Limited | Agriculture & fishing | 1987 |
| E.C.T. (Conyer Conservation) Limited | Professional & scientific | 2010 |
| Electron Electrical Contractors Ltd | Construction | 2013 |
| Elite Timber Solutions Ltd | Construction | 2016 |
| Energetical Consultancy Limited | Professional & scientific | 2014 |
| FAB Architecture Limited | Construction | 2008 |
| Flossie Flowers Ltd | Wholesale & retail | 2024 |
| Forests Philanthropy Action Network | Professional & scientific | 2009 |
| George on High Limited | Real estate | 2009 |
| George on RYE Limited | Hospitality | 2004 |
| Greyfriars Events Ltd | Hospitality | 2023 |
| Griffins Electrical Services Ltd | Construction | 2017 |
| Happy Mallows Ltd. | Manufacturing | 2018 |
| House Rental North West Ltd | Hospitality | 2024 |
| Hurisktic Limited | Information & communication | 2011 |
| Ivywyn Limited | Real estate | 2025 |
| J J S Maintenance Solutions Limited | Other services | 2013 |
| Jack Merricks Farms (RYE) Limited | Agriculture & fishing | 1960 |
| Jake S Bryant Ltd | Construction | 2022 |
| Jules Property Solutions Ltd | Real estate | 2025 |
| K Select Security Consultants Ltd | Administrative & support | 2021 |
| Kameo Hotel Limited | Hospitality | 2023 |
| Lead Assure Ltd | Professional & scientific | 2023 |
| Lisa Mcgee Ltd | Wholesale & retail | 2025 |
| Maclife Ltd | Construction | 2025 |
| Marasby Holdings Ltd | Wholesale & retail | 2026 |
| Matthew Halsall Design Studio Ltd | Professional & scientific | 2018 |
| Morgan Buckley Properties Ltd | Real estate | 2025 |
| Morpar Limited | Real estate | 2002 |
| ON the Ball Plumbing & Heating Ltd | Construction | 2024 |
| Purvis Family Estates Ltd | Real estate | 2024 |
| Redrock Reputation Limited | Professional & scientific | 2025 |
| Ruby RYE Limited | Professional & scientific | 2006 |
| RYE Auction Galleries Ltd | Wholesale & retail | 2013 |
| Samantha Burchett Ltd | Wholesale & retail | 2025 |
| SDB Consulting Services Ltd | Professional & scientific | 2007 |
| Sea Breeze Accounting Ltd | Professional & scientific | 2019 |
| Stylemind Technology Ltd | Information & communication | 2025 |
| Summit Construction Group Ltd | Construction | 2025 |
| Sutton Industrial Park Limited | Real estate | 1989 |
| Tecbiome Limited | Professional & scientific | 2016 |
| THE COT HL Ltd | Hospitality | 2023 |
| THE Vault Retail Ltd | Wholesale & retail | 2025 |
| THE Vault Warehouse Ltd | Wholesale & retail | 2026 |
| Trainmaster Limited | Arts & recreation | 2019 |
| Truebuild Contractors Ltd | Construction | 2020 |
| Vital CD Limited | Professional & scientific | 2026 |
| Voltline Network Limited | Information & communication | 2025 |
| Wardvail Limited | Financial services | 2017 |
| Weald Fencing Products Limited | Professional & scientific | 2009 |
| Weblam Property Ltd | Real estate | 2025 |
| Westgate Hotel Limited | Hospitality | 2009 |
| Westgate Towers Limited | Real estate | 2009 |
| Whiskers & Withers Ltd | Other services | 2025 |
| Winchelsea Beach Angling Limited | Wholesale & retail | 2025 |
| Winchelsea Farm Kitchen Limited | Hospitality | 2024 |
| Winchelsea Lodge Ltd | Hospitality | 2023 |
| Xtreme EMS UK Ltd | Health & social care | 2024 |
| Yuchai UK Ltd | Construction | 2021 |
| Zeus Maintenance Limited | Construction | 2024 |