| A & J Fiskin Joinery Limited | Real estate | 2006 |
| Alphamax Systems Limited | Information & communication | 2006 |
| Ardwell Estates Limited | Real estate | 1934 |
| Auchengallie Farm | | 1992 |
| Baltier Farming Company Limited | Agriculture & fishing | 2006 |
| Caird Leisure Limited | Hospitality | 2005 |
| Calgow Transport Limited | Transport & logistics | 2000 |
| Dourie Holdings Limited | Financial services | 2015 |
| Drummoral Farmers | | 2002 |
| Eric Hyslop, Limited | Construction | 1964 |
| Fred Macpherson & Co Meikle Laight Farms | | 1997 |
| Galdenoch Farmers | | 1995 |
| Glenhead Developments Limited | Agriculture & fishing | 2006 |
| Home & Gardens Limited | Wholesale & retail | 2005 |
| Hugh Ramsay Farms Limited | Agriculture & fishing | 1984 |
| Isle of Whithorn Leisure Limited | Real estate | 1974 |
| J & W Cowan Limited | Agriculture & fishing | 2006 |
| James R Ferguson Limited | Agriculture & fishing | 2005 |
| John Mccolm Ltd | Agriculture & fishing | 2014 |
| Kinnera Homes Limited | Construction | 2003 |
| Knock Farmers | | 1995 |
| M L Wilson Ltd | Education | 2012 |
| Macss Ltd | Administrative & support | 2025 |
| Mahaar Farm | | 1992 |
| Marr Financial Services Ltd | Financial services | 2004 |
| Martin Jolly Truck Hire Limited | Transport & logistics | 1989 |
| Monreith Estate (Overseas) Company | Construction | 1974 |
| Portequip Limited | Manufacturing | 1995 |
| Rowan Glen Dairy Products Limited | Wholesale & retail | 2001 |
| Ryan Construction (Stranraer) Ltd | Construction | 2006 |
| S.W. Plant (Property) Limited | Real estate | 2000 |
| SDS Spares LLP | | 2006 |
| Steam Packet Hotel Co Limited(The) | Hospitality | 1983 |
| THE Dourie Farming Company Limited | Agriculture & fishing | 1954 |
| Torhousemuir Farming Company Limited | Agriculture & fishing | 2007 |
| Upper Barr | Agriculture & fishing | 2013 |
| Vogue Precision Windows Limited | Construction | 1987 |
| W. J. Salfenmoser Limited | Construction | 2006 |
| W.M. Hutchison (LOW Mains) Limited | Agriculture & fishing | 1983 |
| West END Building Services Limited | Wholesale & retail | 1993 |
| Wild Foods of Scotland Limited | Manufacturing | 1991 |