| 54LABS Ltd | Information & communication | 2025 |
| Academic Tutors Limited | Administrative & support | 2021 |
| Aide Associates Ltd | Professional & scientific | 2021 |
| Artsylaser Limited | Manufacturing | 2019 |
| Aspire Learning Trust (Whittlesey) | Education | 2012 |
| Bell OPS Ltd | Professional & scientific | 2017 |
| BHB Building Supplies Limited | Wholesale & retail | 2018 |
| Bradstock Limited | Wholesale & retail | 2024 |
| Bridges Fire & Security Limited | Administrative & support | 2014 |
| C.J. Fountain & SON Ltd | Agriculture & fishing | 1979 |
| Carbon Property Investments Limited | Real estate | 2015 |
| Creative Construction Projects Limited | Construction | 2016 |
| Curry Cottage Z H Ltd | Hospitality | 2025 |
| D7 Nail and Beauty Lounge Limited | Other services | 2013 |
| Dive in Limited | Wholesale & retail | 1991 |
| DP Direct Services Ltd | Construction | 2023 |
| Dynamic Consultancy Solutions Limited | Information & communication | 2024 |
| EA Drywall Ltd | Construction | 2024 |
| Easeway Medicare UK Limited | Health & social care | 2025 |
| Edan Enterprises Limited | Wholesale & retail | 2011 |
| Electric Cable Co. (Great Britain) Limited(The) | Wholesale & retail | 1982 |
| Elisa Cafe Ltd | Hospitality | 2025 |
| Fenland First AID Services Limited | Education | 2010 |
| Fenlec Limited | Construction | 2000 |
| Finance Intelligence Ltd | Wholesale & retail | 2023 |
| Fresh Software Limited | Information & communication | 2004 |
| GRH Security Ltd | Administrative & support | 2022 |
| GS Attah Limited | Health & social care | 2021 |
| Gymkit Limited | Extraterritorial | 2008 |
| Gymkit UK Limited | Extraterritorial | 2008 |
| IN the Future Limited | Wholesale & retail | 2015 |
| J and T Thory Holdings Ltd | Financial services | 2025 |
| J.C.Green & Sons (Coates)Limited | Agriculture & fishing | 1958 |
| Jake Electrical Services Ltd | Construction | 2018 |
| Jesmani Limited | Wholesale & retail | 2021 |
| Kebz Ltd | Hospitality | 2011 |
| Lopin Limited | Hospitality | 2020 |
| Marit Limited | Other services | 2017 |
| Market 12 Limited | Hospitality | 2024 |
| Market Kebab Limited | Hospitality | 2019 |
| Meridian Farms Limited | Agriculture & fishing | 2012 |
| MHZ DEV Ltd | Information & communication | 2026 |
| Michael LHC Locum Limited | Health & social care | 2025 |
| Nesw Logistics Ltd | Transport & logistics | 2024 |
| Newell Scaffolding Limited | Construction | 2011 |
| Olly's Kitchens Limited | Construction | 2004 |
| Orchard House (Whittlesey) Limited | Hospitality | 2002 |
| OUT of Afrika Private Ltd | Hospitality | 2025 |
| P J Thory Holdings Limited | Real estate | 2014 |
| Parmatma Company Limited | Hospitality | 2024 |
| Peterborough Design Limited | Professional & scientific | 2023 |
| Peterborough Headspa Limited | Other services | 2025 |
| RAI and RAI General Builders Limited | Construction | 2020 |
| RF Electrical Services Limited | Construction | 2024 |
| RJ Community CIC | Education | 2026 |
| Robert German Carpenter & Joiner Limited | Manufacturing | 2022 |
| Royston Farms Holdings Limited | Financial services | 2024 |
| SKF Developments Ltd | Construction | 2019 |
| SRG (Whittlesey) Ltd | Wholesale & retail | 2023 |
| Storeys Accident Repair Centre Ltd | Wholesale & retail | 2014 |
| Tazmanian Limited | Transport & logistics | 2019 |
| THE Boat Inn (Whittlesea) Limited | Hospitality | 2002 |
| THE Redeemed Christian Church of GOD Restoration Court Peterborough | Other services | 2023 |
| THE Straw Bear B&B Limited | Extraterritorial | 2018 |
| Tyche Bliss Ltd | Information & communication | 2023 |
| Vakarian Vehicle Services Ltd | Wholesale & retail | 2020 |
| Violet Transport Ltd | Transport & logistics | 2015 |