| 1:M Data Services Ltd | Information & communication | 2025 |
| 23 Charledown Close Limited | Households | 2021 |
| 29 Bell Street Management Company (Whitchurch) Limited | Real estate | 1989 |
| 2BAPPY Ltd | Wholesale & retail | 2025 |
| 2TANNED Ltd | Other services | 2026 |
| 3 D Technical Services Limited | Professional & scientific | 2009 |
| 3DCLEVER Ltd | Administrative & support | 2019 |
| 3DWOOL Ltd | Wholesale & retail | 2024 |
| 3FD Ltd | Professional & scientific | 2019 |
| 4 Jays Limited | Real estate | 2003 |
| 4IR Technical Services Ltd | Professional & scientific | 2024 |
| 4M Holdings Limited | Financial services | 2019 |
| 4M Portable Buildings Hire Limited | Administrative & support | 1999 |
| 4M Portable Buildings Limited | Extraterritorial | 1992 |
| 4MOST Developments Limited | Real estate | 2012 |
| 4X4 Fabrication Limited | Wholesale & retail | 2018 |
| 5 Point 5 Acre Limited | Real estate | 2015 |
| 52/54 London Street, Whitchurch, Management Company Limited | Real estate | 1999 |
| @29 Café Limited | Wholesale & retail | 2017 |
| A & G Llews Limited | Financial services | 2026 |
| A & HF Construction Ltd | Construction | 2022 |
| A & R Hair Ltd | Arts & recreation | 2019 |
| A Bate Building Contractor Limited | Construction | 2010 |
| A C Owen Agri Contracting Ltd | Agriculture & fishing | 2025 |
| A G H Contract Services Limited | Construction | 2004 |
| A M Pavier Plumbing and Heating Limited | Construction | 2011 |
| A M Systems (Engineering) Limited | Construction | 1997 |
| A Plus Contracts Ltd | Construction | 2018 |
| A Precious Life (First AID) Limited | Education | 2016 |
| A VON Dinther Farrier Service Limited | Agriculture & fishing | 2011 |
| A&R Cocis Ltd | Health & social care | 2021 |
| A.K.L. Bearing & Power Transmission Company Limited | Wholesale & retail | 1988 |
| A.O. Plastering and Rendering Limited | Construction | 2025 |
| A2B Motorhome Services Ltd | Wholesale & retail | 2026 |
| Aardvark Cleaning Services Ltd | Administrative & support | 2019 |
| Aardvark Heat & Water Ltd | Construction | 2012 |
| AB Powder Products Limited | Manufacturing | 2019 |
| Abacus Plumbing & General Builders Ltd | Construction | 2007 |
| Abbie's Pets & Exotics Ltd | Other services | 2019 |
| ABD2 Limited | Real estate | 2015 |
| Access Drone Ltd | Agriculture & fishing | 2024 |
| Accm Ltd | Real estate | 2016 |
| Ace Locks & Security Ltd | Construction | 2021 |
| Acorn Wood Care Limited | Health & social care | 2025 |
| Acorn Wood Education Limited | Education | 2025 |
| ACS Timber Haulage Ltd | Transport & logistics | 2022 |
| Activecare Limited | Health & social care | 1995 |
| Acumen Eight Limited | Transport & logistics | 2019 |
| AD Frameworks Ltd | Administrative & support | 2022 |
| Adaptra Ltd | Professional & scientific | 2025 |
| ADD Design Limited | Information & communication | 2024 |
| Adept Precision Sheetmetal Limited | Manufacturing | 1986 |
| Adnoise Limited | Information & communication | 2017 |
| Adorn Aesthetics Hampshire Ltd | Other services | 2020 |
| Advantage Media Group Ltd | Professional & scientific | 2020 |
| Advitel Limited | Information & communication | 1994 |
| Adviweb Limited | Other services | 2002 |
| AEB BI Limited | Professional & scientific | 2023 |
| Aecer Ltd | Professional & scientific | 2015 |
| Aegir Engineering & Consultancy Services Limited | Manufacturing | 2006 |
| Agden Hall Limited | Extraterritorial | 2023 |
| Agden House Farm Ltd | Agriculture & fishing | 2025 |
| Agri-Puls Whitchurch LLP | | 2010 |
| Agriponics Global Research Industries Limited | Agriculture & fishing | 2021 |
| Agsafe Ltd | Professional & scientific | 2020 |
| AI Future Technologies Limited | Information & communication | 2025 |
| Air Accounting Limited | Professional & scientific | 2016 |
| AJ Global Enterprises Limited | Wholesale & retail | 2026 |
| AJD Cooper Limited | Administrative & support | 2005 |
| Albright (Engineers) Limited | Manufacturing | 1963 |
| Albright Dene Ltd | Construction | 2011 |
| Albright International Limited | Manufacturing | 1976 |
| Albrighton Properties Ltd | Real estate | 2004 |
| Alchemette Chester Ltd | Real estate | 2021 |
| Alchemette Chester TC Ltd | Real estate | 2026 |
| Alchemette Properties Ltd | Professional & scientific | 2012 |
| Alderford Lake Ltd | Arts & recreation | 2022 |
| Alfresco Kitchen at Hadley Park Limited | Hospitality | 2026 |
| Alimenta Limited | Information & communication | 2000 |
| ALL Interactive Ltd | Wholesale & retail | 2008 |
| ALL Tail Lifts Limited | Manufacturing | 2017 |
| ALL4DAIRY Ltd | Information & communication | 2022 |
| ALL4FEET Ltd | Information & communication | 2016 |
| Allen Family Farm Limited | Agriculture & fishing | 2014 |
| Allnutt Properties Ltd | Real estate | 2023 |
| Allorc Consulting Ltd | Information & communication | 2024 |
| ALM Logistics Ltd | Transport & logistics | 2025 |
| Almar Construction Ltd | Construction | 2015 |
| ALT Space Studio Limited | Arts & recreation | 2022 |
| Alternative Meats Limited | Wholesale & retail | 2001 |
| AM Taxation Limited | Professional & scientific | 2010 |
| Amanda June Jewellery Ltd | Manufacturing | 2024 |
| Ambrasana Limited | Other services | 2021 |
| Americold Whitchurch Ltd | Transport & logistics | 1981 |
| Amersham GAS Services Limited | Construction | 2019 |
| AMW Consultants Limited | Professional & scientific | 2022 |
| Anchorage Properties U.K. Limited | Real estate | 2023 |
| Andistan Consulting Ltd | Health & social care | 2022 |
| Andistan Limited | Construction | 2019 |
| Andre Hartford Limited | Manufacturing | 1975 |