| 888 Detailing & Valeting Ltd | Administrative & support | 2022 |
| Aceo Limited | Manufacturing | 1999 |
| Adrian Wintle Racing Limited | Arts & recreation | 2015 |
| Apex Gloucester Ltd | Hospitality | 2017 |
| Carringtonlime Heritage Skills Training C.I.C. | Construction | 2020 |
| Chaxhill Developments Limited | Real estate | 2004 |
| Coastline Property Ventures Ltd | Real estate | 2025 |
| Compressed Air Consultants Limited | Wholesale & retail | 1984 |
| Cool Hire Limited | Administrative & support | 2018 |
| D&R Catering Limited | Hospitality | 2026 |
| Digital Draught Limited | Wholesale & retail | 2024 |
| Elton Racing Ltd | Arts & recreation | 2014 |
| Gemcc Properties Ltd | Real estate | 2022 |
| Gloucester Car Boot Limited | Wholesale & retail | 2016 |
| Gooburg Enterprises Limited | Manufacturing | 2022 |
| Ironside Trading Ltd | Wholesale & retail | 2026 |
| JAH Consultancy Services Ltd | Professional & scientific | 2019 |
| Jred Limited | Hospitality | 2022 |
| JWP Cotswold Plasterwork Limited | Manufacturing | 2024 |
| Keenanclean Limited | Water & waste | 2016 |
| Killean Estate Ltd | Hospitality | 2012 |
| Kingsman Equine LLP | | 2017 |
| Laguna's Spitfire Legacy C.I.C. | Education | 2019 |
| Ledbury Car Boot Limited | Wholesale & retail | 2021 |
| Marshall Andrews Limited | Information & communication | 2007 |
| Mews Auction Rooms Limited | Wholesale & retail | 2017 |
| MJW Catering Services Limited | Hospitality | 2024 |
| Murray Mcdavid Whisky Limited | Extraterritorial | 2013 |
| Nipper Designs Ltd | Information & communication | 2011 |
| OFT Training Solutions Limited | Other services | 2010 |
| Paydays Bureau Limited | Professional & scientific | 2010 |
| Perry Orchard (Gloucester) Management Company Limited | Households | 1976 |
| Pinktoucanco Ltd | Wholesale & retail | 2021 |
| Pjbees Enterprises Ltd | Manufacturing | 2025 |
| Postance Ltd | Professional & scientific | 2021 |
| Premier Lane Vehicle Services Limited | Wholesale & retail | 2025 |
| PSK Plumbing and Heating Ltd | Energy | 2016 |
| PSW Metals Limited | Water & waste | 2005 |
| Pump and Cover Apparel Limited | Wholesale & retail | 2025 |
| Pure Panelling Ltd | Manufacturing | 2024 |
| R & J Hewlett Limited | Agriculture & fishing | 1991 |
| River Wood Cottage Limited | Real estate | 2025 |
| Roberts Construction & Landscape Limited | Construction | 2019 |
| Sinobus Ltd | Real estate | 2013 |
| Skhc Properties Ltd | Professional & scientific | 2007 |
| SNR Services (Glos) Limited | Wholesale & retail | 2009 |
| SNR Services (Ross) Limited | Wholesale & retail | 2023 |
| SNR Services (UK) Limited | Wholesale & retail | 2008 |
| Squid Bookkeeping Ltd | Professional & scientific | 2023 |
| THE Cooks' Fish & Game Limited | Extraterritorial | 2007 |
| THE Four Wall Club Ltd | Hospitality | 2024 |
| THE Heat Pump Centre Limited | Manufacturing | 2007 |