| 3-56 Media Limited | Information & communication | 2011 |
| 6THSENSEUK Ltd | Professional & scientific | 2025 |
| 762 Edin Ltd. | Transport & logistics | 2003 |
| A1 Plumbing & Drainage Scotland Ltd | Construction | 2008 |
| Above and Beyond Cleaning Ltd | Administrative & support | 2011 |
| Accessible Housing Scotland Limited | Real estate | 2024 |
| Acrecoast Limited | Administrative & support | 2002 |
| ACX Software Limited | Real estate | 1996 |
| Adharola Limited | Transport & logistics | 2020 |
| Admos Specialists Ltd | Construction | 2024 |
| Aksi Medicolegal Limited | Administrative & support | 2016 |
| Alderside Property Ltd | Real estate | 2018 |
| Alexec Taxis Limited | Professional & scientific | 2000 |
| Alistaire Patterson Haulage Limited | Transport & logistics | 2011 |
| Alister and Kathleen Laird Limited | Administrative & support | 1990 |
| Allways Travel Group Ltd | Administrative & support | 2025 |
| Amtec Electrical Systems Ltd | Manufacturing | 2024 |
| AN Torr Consulting Ltd | Other services | 2019 |
| Aspitech Limited | Professional & scientific | 2019 |
| ATA North Ltd | Transport & logistics | 2025 |
| Bead Properties Scotland Limited | Real estate | 2022 |
| Bonting Ltd | Professional & scientific | 2025 |
| Border MIX Ltd | Construction | 2006 |
| Bordlands Ltd | Information & communication | 2024 |
| Cairnburn Limited | Real estate | 2000 |
| Cajb Consultant Ltd | Professional & scientific | 2022 |
| CAL People Limited | Administrative & support | 2017 |
| Calm Hair and Beauty Limited | Other services | 2013 |
| Carriden Boatyard Limited | Manufacturing | 1992 |
| Castle Craig Hospital Limited | Health & social care | 1988 |
| Cauldstane Ltd | Professional & scientific | 2016 |
| Cavalry Garage Services Limited | Wholesale & retail | 2019 |
| Cavalry Transport Limited | Transport & logistics | 2012 |
| Chalkmark Management Consulting Ltd. | Professional & scientific | 2007 |
| Change Works FH Limited | Professional & scientific | 2009 |
| Circuit 29 Ltd | Professional & scientific | 2025 |
| Citi Pro Ltd. | Real estate | 2016 |
| Clockwork Transformation Limited | Information & communication | 2011 |
| Craft Butchers Ltd | Wholesale & retail | 2026 |
| Credit Connect Group Limited | Real estate | 2023 |
| D & a Leisure (Scotland) Ltd | Hospitality | 2004 |
| Derek Alexander Limited | Construction | 2003 |
| Descartes Consulting Limited | Professional & scientific | 2023 |
| Dickson Mcgregor Ltd | Real estate | 2019 |
| Distilled Experience Ltd | Manufacturing | 2018 |
| DMC Web Services Limited | Information & communication | 2005 |
| Duncan Davidson Welding & Fabrication Ltd | Manufacturing | 2024 |
| Ecospark Energy Solutions Ltd | Construction | 2023 |
| Edinburgh Business Coaching Limited | Professional & scientific | 2005 |
| Edinburgh Micro Diggers Ltd | Professional & scientific | 2022 |
| Edusy Limited | Extraterritorial | 2022 |
| Erlend Milne and Company Limited | Other services | 2023 |
| Evergrow Ltd | Agriculture & fishing | 2016 |
| Fairview Leisure (Scotland) Ltd | Hospitality | 2022 |
| Family History Scotland Ltd | Other services | 2019 |
| Faodail Landscapes Ltd | Administrative & support | 2021 |
| Farmers Arms (Thornhill) Limited | Hospitality | 2023 |
| Ferliehald Ltd | Professional & scientific | 2023 |
| Ferniehaugh Farms | | 1991 |
| Fiona Harvey Consulting Limited | Professional & scientific | 2025 |
| FLY Mobile Apps Limited | Information & communication | 2013 |
| Flyingscool Ltd | Education | 2019 |
| Forever Timber Ltd | Other services | 2026 |
| Forr Data Ltd | Information & communication | 2025 |
| Fusion Futsal Ltd | Arts & recreation | 2024 |
| G S Chapman Ltd | Manufacturing | 2012 |
| G S Howieson (Winter Services) Limited | Transport & logistics | 2015 |
| Garvald Home Farm Limited | Agriculture & fishing | 1988 |
| Garvald West Linton Ltd. | Health & social care | 1976 |
| GET My Agent Ltd | Real estate | 2020 |
| Gili Pickle Ltd | Hospitality | 2024 |
| Glasgow Global Therapy Limited | Health & social care | 2016 |
| Glencoe Cottages Management Company Ltd | Hospitality | 2023 |
| Glenrath EGG Products Limited | Manufacturing | 2013 |
| Glenrath Farms Ltd. | Agriculture & fishing | 1965 |
| Green Summit Sustainability Ltd | Professional & scientific | 2025 |
| Gumpa Ltd | Agriculture & fishing | 2021 |
| Gyroscope Ltd | Professional & scientific | 2022 |
| Happy Elephant Candles Ltd | Manufacturing | 2020 |
| Harderwijk Developments Limited | Real estate | 2017 |
| HD Brinck Investments Limited | Administrative & support | 2022 |
| Hot House Sauna Group Ltd | Arts & recreation | 2026 |
| Hulk BIO Ltd | Professional & scientific | 2023 |
| Hyper Baric Oxygen Treatment Trust | Health & social care | 1996 |
| Information Browser Limited | Information & communication | 1995 |
| International Resources and Recycling Institute | Professional & scientific | 2002 |
| J & a Weir Farming Limited | Agriculture & fishing | 2010 |
| John G Moore Engineering Ltd | Construction | 2012 |
| John Swan Garage Services Limited | Wholesale & retail | 2008 |
| Joint Ideas Ltd. | Information & communication | 2002 |
| K L Electric Ltd | Construction | 2008 |
| K777DAC Limited | Professional & scientific | 2009 |
| Kerr Catering Ltd | Hospitality | 2025 |
| Keypro Limited | Professional & scientific | 2001 |
| Ladyurd Steading Ltd | Hospitality | 2018 |
| Lamancha and District Community Association | Other services | 2004 |
| Lamancha Consulting Limited | Professional & scientific | 2016 |
| Landlord Catering Ltd | Hospitality | 2017 |
| Leaders for Leadership Ltd. | Professional & scientific | 2005 |
| Linton Park Property Investments Ltd | Real estate | 2018 |