| A.J. Mcalpine Construction Ltd. | Construction | 2012 |
| Abelresources Ltd | Construction | 2017 |
| Ace.I Integration Limited | Information & communication | 2026 |
| Alex Adam & SON Limited | Construction | 2014 |
| Alligator Roofing Ltd | Construction | 2026 |
| Am-Ex (American Exports) Limited | Hospitality | 1981 |
| Amethyst Global Ltd | Professional & scientific | 2020 |
| Angus Cochran Patrick Ltd | Construction | 2012 |
| ANM Caravan Services Ltd | Manufacturing | 2023 |
| Ardneil Farm | | 1984 |
| Ashrona Power Systems Limited | Energy | 2007 |
| Avondale Estates Ayrshire Ltd | Real estate | 2025 |
| Ayrshire Intelligence Ltd | Professional & scientific | 2025 |
| Ayrshire Letting and Sales Ltd | Real estate | 2014 |
| Ayrshire Property Solutions Ltd | Real estate | 2008 |
| Bamber Property Services Limited | Real estate | 2024 |
| Barbour Structures Ltd | Construction | 2019 |
| Barony Retail Ltd | Wholesale & retail | 2024 |
| BE Seen Local Ltd | Professional & scientific | 2019 |
| Blackshaw Barns Ltd | Manufacturing | 2021 |
| Brickhouse Vintage Limited | Other services | 2020 |
| Bridge of Weir Hydro Ltd | Energy | 2026 |
| Brisbane Glen Estate Company, Limited | Agriculture & fishing | 1933 |
| BRM Construction Management Limited | Construction | 2014 |
| Buzcart Limited | Energy | 2014 |
| C. M. International Ltd | Wholesale & retail | 2022 |
| Cafe Willow Ltd | Hospitality | 2025 |
| Carbon Technologies Limited | Professional & scientific | 2024 |
| Carlung Estate Company Limited | Agriculture & fishing | 1906 |
| CGR Energy Ltd. | Professional & scientific | 2015 |
| Chapelhill Developments Limited | Construction | 2021 |
| Coast Estate Agents Ltd | Real estate | 2026 |
| Columba Retail Limited | Wholesale & retail | 2016 |
| Core Property Solutions (Ayrshire) Limited | Real estate | 2014 |
| Custom Capital Consulting Ltd | Financial services | 2026 |
| D B Forestry Limited | Agriculture & fishing | 2020 |
| Daniel Smith Sailing Ltd | Arts & recreation | 2019 |
| Day JOB Studios Limited | Information & communication | 2023 |
| DMD Labour Ltd | Construction | 2018 |
| Doura Electric Ltd | Construction | 2008 |
| DVM Management in Construction Ltd | Construction | 2018 |
| Earth Source Clyde Copper Limited | Professional & scientific | 2021 |
| ECG Home Improvements Limited | Construction | 2016 |
| Ecomm-Workstreams Limited | Professional & scientific | 2023 |
| Elevated Consulting Ltd | Professional & scientific | 2023 |
| EPM Mechanical Limited | Construction | 2026 |
| Event Ace Limited | Information & communication | 2019 |
| Forth Vision Limited | Administrative & support | 2017 |
| Forthside Consulting Limited | Professional & scientific | 2013 |
| Forthside Images Limited | Professional & scientific | 2014 |
| Fraser Allan Sports Management Limited | Professional & scientific | 2022 |
| Friends of Portencross Castle | Education | 1998 |
| Fritz and Heidi Properties Limited | Real estate | 2016 |
| G2EM Property Limited | Construction | 2015 |
| Gaign Strategic Fundraising Ltd | Professional & scientific | 2025 |
| GGH8 Ltd | Professional & scientific | 2025 |
| Glenbryde Limited | Wholesale & retail | 2015 |
| Gmth Solutions Ltd | Households | 2023 |
| Growth Venture Club Ltd | Financial services | 2022 |
| Haakon Composites and Coatings Limited | Manufacturing | 2018 |
| Hackcasts Ltd | Information & communication | 2026 |
| Happy Developments & Refurbishments Ltd | Real estate | 2025 |
| Hawthorn & Horten Ltd | Information & communication | 2024 |
| HBA Rigging Ltd | Construction | 2026 |
| Helix North Ltd | Information & communication | 2015 |
| Hodge Solutions Limited | Other services | 2020 |
| Iain Plimbley Ltd | Professional & scientific | 2017 |
| Identified Ltd | Information & communication | 2019 |
| James Gray Electrical Ltd | Extraterritorial | 2023 |
| Janine Marriott Ltd | Information & communication | 2024 |
| JB Padel Ltd | Education | 2022 |
| Jennifer Reekie Locum Services Ltd | Professional & scientific | 2018 |
| JMC Financial Services Limited | Financial services | 2003 |
| JMC Holdings (Scotland) Ltd | Financial services | 2024 |
| K N Technical Services ( Scotland ) Limited | Professional & scientific | 2012 |
| Kamboh 1 Ltd | Hospitality | 2019 |
| L.A.H. Property Solutions Limited | Real estate | 2017 |
| L.A.H. Travel Limited | Administrative & support | 2004 |
| Ladybird Designs CIC | Professional & scientific | 2026 |
| Lead Alert Ltd | Construction | 2014 |
| LML Removals & Logistics Ltd | Transport & logistics | 2026 |
| Lochwinnoch Developments Ltd | Energy | 2020 |
| Loretta Doyle Sports Management Limited | Professional & scientific | 2024 |
| M.M.S. Scotland Limited | Professional & scientific | 1999 |
| Macdonald Highland Ponies Limited | Agriculture & fishing | 2023 |
| MAE Rose Ltd | Construction | 2022 |
| Makir Associates Ltd | Health & social care | 2023 |
| Mcatee Properties Limited | Households | 2012 |
| Mccrindle Group Limited | Real estate | 1966 |
| Mccrindle Tooling Limited | Extraterritorial | 1975 |
| Mcgav X Ltd | Real estate | 2024 |
| Meadowfoot Ecom Ltd | Manufacturing | 2022 |
| Media World Limited | Information & communication | 1989 |
| MGN Retail Largs Limited | Wholesale & retail | 2026 |
| Mind Health Hypnotherapy Limited | Health & social care | 2018 |
| Mirapicado Group Limited | Professional & scientific | 2025 |
| Mirapicado Ltd | Information & communication | 2025 |
| Modes Emporium Limited | Wholesale & retail | 2023 |
| Molly Leisure Ltd | Hospitality | 2021 |
| Move 2 Lettings and Sales Limited | Administrative & support | 2012 |