| 10MEG Consulting Ltd | Information & communication | 2015 |
| A23 Education Ltd | Arts & recreation | 2013 |
| A23 Football Group Ltd | Arts & recreation | 2019 |
| Aberlady Enterprises Ltd | Real estate | 2012 |
| Adam Windmill Ltd | Information & communication | 2007 |
| Adpulsum Consulting Ltd | Professional & scientific | 2017 |
| Agile HR Services Ltd | Professional & scientific | 2023 |
| AGN Corporate Law Limited | Professional & scientific | 2011 |
| Air+D Artificial Intelligence Retail & Design Ltd | Information & communication | 2017 |
| Align Left Limited | Professional & scientific | 2008 |
| ALL Being Well Nutrition Limited | Health & social care | 2023 |
| Amanda Statham Coaching and Development Ltd | Professional & scientific | 2014 |
| Anglameric Properties (Aylesbury) Limited | Administrative & support | 1975 |
| APM Internet Limited | Information & communication | 2002 |
| AS WE Please Ltd | Professional & scientific | 2017 |
| Autism Teaching Company Limited | Education | 2018 |
| Avgen Capital Partners Limited | Construction | 2020 |
| Ayerst Consulting Limited | Professional & scientific | 2021 |
| Back Street Courtyard Limited | Real estate | 2022 |
| Bakewell Holdings Ltd | Construction | 2022 |
| Bambamsound Limited | Information & communication | 2010 |
| Batts Building and Decorating Ltd | Construction | 2018 |
| Beacons TDP Limited | Professional & scientific | 2012 |
| Bessant Business Support Ltd | Information & communication | 2014 |
| Best Business Software Limited | Professional & scientific | 2007 |
| Better Reuse Limited | Water & waste | 2019 |
| Bgae Ltd | Administrative & support | 2014 |
| Bucks Academy of Sport & Exercise Ltd | Arts & recreation | 2018 |
| Bucks Sport Academy Ltd | Arts & recreation | 1997 |
| Castle Park Solutions Limited | Professional & scientific | 2009 |
| Cesl Holdings Ltd | Financial services | 2023 |
| CHB (Milton Keynes) Ltd | Wholesale & retail | 2011 |
| Checkify Ltd | Information & communication | 2021 |
| Chiltern Artisan Limited | Manufacturing | 2016 |
| Chiltern EYE Surgery Ltd | Health & social care | 2016 |
| Chiltern Stores Limited | Wholesale & retail | 2005 |
| Chiltern Veterinary Centre Ltd | Professional & scientific | 2025 |
| Cloud Top Consulting Ltd | Information & communication | 2019 |
| CMS Catalyst Ltd | Real estate | 2021 |
| Corixus Ltd | Administrative & support | 2011 |
| CR Builders (Wendover) Limited | Construction | 2014 |
| Dani Smallbone Consulting Limited | Professional & scientific | 2019 |
| DB5 Solutions Ltd | Professional & scientific | 2013 |
| Dbwaanders Group Limited | Financial services | 2018 |
| Domino Equipping Solutions | Other services | 1998 |
| Duke Road Freehold Limited | Households | 2019 |
| Eagleton Haematologist Ltd | Health & social care | 2016 |
| Easy Balloons Limited | Other services | 2010 |
| Elite Property Management Team Ltd | Real estate | 2025 |
| Ellesborough Investments Limited | Real estate | 2006 |
| EP Trans Ltd | Transport & logistics | 2018 |
| EVO Industrial Ltd | Construction | 2020 |
| Exclusive Equestrian Limited | Wholesale & retail | 2001 |
| F T B Building Solutions Ltd | Wholesale & retail | 2021 |
| Flyte Creative Media Limited | Information & communication | 2018 |
| Forgham Investments Limited | Construction | 2016 |
| Fullerene Ltd | Administrative & support | 2018 |
| G Williams Builders Ltd | Construction | 2017 |
| G.R.E.P.H. Engineering Ltd | Manufacturing | 2023 |
| G2H Consulting Limited | Professional & scientific | 2022 |
| Garden Soul Ltd | Administrative & support | 2024 |
| Granger Plant Hire Ltd | Administrative & support | 2025 |
| Greenfingers Charity | Administrative & support | 1999 |
| Helen Boardman Limited | Professional & scientific | 2018 |
| HOH Oilfield Services Limited | Mining & quarrying | 1998 |
| Impresario Partners International Ltd | Information & communication | 2022 |
| Individual Consulting Limited | Professional & scientific | 2011 |
| Institute of Carpenters (the) | Other services | 1903 |
| Intuitav Limited | Information & communication | 2018 |
| Iris Gallery Ltd | Professional & scientific | 2024 |
| Jenova Trading Limited | Wholesale & retail | 2017 |
| JFS Property and Project Management Limited | Professional & scientific | 2019 |
| JFS Property Services Limited | Construction | 2016 |
| John Colet School | Education | 2011 |
| K2H Limited | Administrative & support | 2004 |
| Kenali Design Ltd | Wholesale & retail | 2021 |
| KIM Huggins Limited | Professional & scientific | 2014 |
| Kirsten Fraser Limited | Hospitality | 2016 |
| L Barwick Ltd | Professional & scientific | 2017 |
| Labc Acoustics Ltd | Extraterritorial | 2010 |
| Laura Croker PA Ltd | Administrative & support | 2018 |
| Lawnhurst Services Limited | Manufacturing | 1998 |
| LE4D Limited | Health & social care | 2008 |
| Lean Business Excellence Limited | Professional & scientific | 2017 |
| Leaps & Bounds (Hertfordshire) Limited | Real estate | 1992 |
| Lesingham Limited | Real estate | 2005 |
| LLD Consulting Ltd | Information & communication | 2012 |
| Manor Drive Developments Limited | Real estate | 2018 |
| Match Mortgages Limited | Financial services | 2022 |
| Matmar Investments Limited | Professional & scientific | 2024 |
| MG Wendover Investments Limited | Real estate | 2019 |
| Moheli Shores Limited | Construction | 2019 |
| MRF Consulting Services Limited | Professional & scientific | 2017 |
| MSP Business Consulting Ltd | Information & communication | 2010 |
| Nb:Bn Ltd. | Professional & scientific | 2018 |
| New House Hale Lane Management Company Limited | Households | 2015 |
| Nicecreation Limited | Information & communication | 2010 |
| Niels Ltd | Information & communication | 2026 |
| Nigel Grace Limited | Professional & scientific | 2016 |
| NKG Homes Limited | Construction | 2019 |