| Eclat Estates Limited | Information & communication | 2023 |
| Eco Branch Ltd | Wholesale & retail | 2022 |
| Eco Redwood Windows Limited | Construction | 2007 |
| Eco Smart Installations Limited | Construction | 2022 |
| Ecomwhole Ltd | Wholesale & retail | 2024 |
| Edamid Ltd | Health & social care | 2024 |
| Edge Internet Limited | Information & communication | 2015 |
| EDM Tewin Ltd | Hospitality | 2024 |
| Educivitas Limited | Information & communication | 2020 |
| EDVIATHIA24/7 Ltd | Health & social care | 2012 |
| Edwin & Harley Property Ltd | Real estate | 2014 |
| EEM Multi Services Ltd | Administrative & support | 2024 |
| Efie Magazine Ltd | Information & communication | 2025 |
| EFS Building Ltd | Construction | 2025 |
| EJ Enterprise 33 Limited | Arts & recreation | 2014 |
| Ekelectrics Limited | Construction | 2015 |
| Elder Engineering (Herts) Limited | Manufacturing | 1980 |
| Eldon Marketing Limited | Information & communication | 2023 |
| Elec Comply Ltd | Construction | 2024 |
| Elevare Health Ltd | Health & social care | 2026 |
| Eleven 92 Lighting Ltd. | Construction | 2017 |
| Elias Empire Ltd | Health & social care | 2019 |
| Elichi Limited | Health & social care | 2016 |
| Elimef Ltd | Administrative & support | 2026 |
| Elis Gift Shop Limited | Wholesale & retail | 2025 |
| Elisa Design Ltd | Professional & scientific | 2021 |
| Elite Architectural Systems Limited | Construction | 2010 |
| Elite Garden Rooms Ltd | Construction | 2024 |
| Elite Management Limited | Real estate | 2026 |
| Ellbee Access Limited | Construction | 2023 |
| Ellingham House Day Nursery Limited | Education | 1995 |
| Elly& Co. Limited | Health & social care | 2014 |
| Elmwood Education Limited | Information & communication | 2013 |
| Elmwood PM Ltd | Construction | 2011 |
| Elmwood Press Limited | Information & communication | 2002 |
| Elmwood RE Ltd | Households | 2012 |
| Elnick UK Ltd | Wholesale & retail | 2008 |
| Elsmin Limited | Professional & scientific | 2025 |
| Elstree Precision Company Limited | Manufacturing | 1961 |
| Emerald Commercial Electrical Ltd | Construction | 2018 |
| Emilio Electric Ltd | Construction | 2014 |
| Emily Williams Design Ltd | Professional & scientific | 2025 |
| Emma Hurley Photography Ltd | Other services | 2014 |
| Empowerment Solutions (Herts) Limited | Education | 2013 |
| Empress Medical Ltd | Health & social care | 2023 |
| ENA Care Group Limited | Health & social care | 2011 |
| ENA Hourly Care Limited | Health & social care | 2009 |
| Enco Products Limited | Hospitality | 1946 |
| Enigma Therapeutics Ltd | Professional & scientific | 2023 |
| Ennex Ltd | Wholesale & retail | 2023 |
| ENP Limited | Health & social care | 2018 |
| Entelechy Enterprises Limited | Wholesale & retail | 2013 |
| Envision Computing Ltd | Information & communication | 2014 |
| Epicurean Hospitality & Event Consultancy Ltd | Hospitality | 2024 |
| Eprint Direct Limited | Information & communication | 1999 |
| Eprint Limited | Financial services | 2024 |
| Equate Advisory Limited | Professional & scientific | 2022 |
| Equinox Clinical Trial Services Ltd | Professional & scientific | 2019 |
| Equity Print Ltd | Manufacturing | 2023 |
| Erom Labs Ltd | Information & communication | 2026 |
| ESA Spread Development Limited | Construction | 2018 |
| Eshelby Strategic Holdings Limited | Financial services | 2025 |
| Esperer Consulting Ltd | Information & communication | 2020 |
| Essancy Limited | Manufacturing | 2009 |
| Essie & Betsy's Ltd | Wholesale & retail | 2022 |
| Essorix Ltd | Information & communication | 2026 |
| Esteem IT Consultancy Ltd | Information & communication | 2020 |
| Estemiki Ltd | Health & social care | 2014 |
| Estilosys Ltd | Information & communication | 2025 |
| Eton Crest Construction Ltd | Construction | 2026 |
| Etrum Healthcare Services Ltd | Health & social care | 2021 |
| Eudemonia Coaching Ltd | Other services | 2020 |
| Eudora Consulting Ltd | Information & communication | 2013 |
| Eudyptes Ltd | Administrative & support | 2017 |
| Eureka UN Ltd | Information & communication | 2012 |
| European Nursing Agency Limited | Administrative & support | 1998 |
| Evangelist Arthur J. Milton Evangelistic Association Limited | Other services | 1967 |
| Evans Tech Solutions Ltd | Administrative & support | 2019 |
| Evara Amore Limited | Other services | 2025 |
| Event Payment Services Limited | Administrative & support | 2021 |
| Event Suite Ltd | Information & communication | 2022 |
| Events Plus Limited | Wholesale & retail | 2024 |
| Ever Berry Events Ltd | Administrative & support | 2025 |
| Everbuild Building Products Limited | Manufacturing | 1994 |
| Evertise Newswire Ltd | Wholesale & retail | 2025 |
| Everwell Labs Limited | Wholesale & retail | 2025 |
| Evoke Tree Houses Ltd | Manufacturing | 2023 |
| Evoke Tree Services Ltd | Agriculture & fishing | 2016 |
| Evolve Fitness Academy Ltd | Other services | 2024 |
| Exact Fire Safety Ltd | Public administration | 2025 |
| Excellence Recruit Limited | Administrative & support | 2017 |
| Executive Grapevine International Limited | Information & communication | 1993 |
| Expo Portable Group Ltd | Financial services | 2024 |
| Exposuisse Ltd | Other services | 2022 |
| Exprocar Ltd | Wholesale & retail | 2014 |
| EYP Store Limited | Wholesale & retail | 2024 |
| Ezenetwork Services Limited | Information & communication | 2007 |
| F44 Investments Limited | Information & communication | 2013 |
| Faber Logic Ltd | Information & communication | 2024 |
| Facial & Oral Surgery Services Ltd | Health & social care | 2014 |