| Twiddy Simmons Limited | Information & communication | 2024 |
| Twin Scales Ltd | Hospitality | 2022 |
| Twisted Recruitment Ltd | Administrative & support | 2025 |
| Two SIX Heave Ltd | Professional & scientific | 2020 |
| Twss Ltd | Information & communication | 2025 |
| Tyche Business Services Limited | Professional & scientific | 2019 |
| Tyger Limited | Real estate | 2008 |
| U Lobster Limited | Hospitality | 2026 |
| UD & Sons Limited | Real estate | 2020 |
| UD (Tottenham Lane) Limited | Construction | 2015 |
| Ujima Ltd | Wholesale & retail | 2012 |
| UK Dental Design Group Limited | Financial services | 2018 |
| UK Dental Pro Ltd | Education | 2023 |
| UK Eagle Properties Limited | Real estate | 2022 |
| UK Epic Services Limited | Wholesale & retail | 2019 |
| UK Fire Door Services Ltd | Construction | 2025 |
| UK Glazing Solutions Limited | Construction | 2017 |
| UK Guild for Integrated,Functional, Quantum, Holistic, Homeopathic, Psychological, Herbal,Naturopathic, Lifestyle, Natural and Innovative Medicine Limited | Health & social care | 2025 |
| UK Hot Tubs Ltd | Construction | 2009 |
| UK Hygienist Ltd | Health & social care | 2020 |
| UK Malayali News Ltd | Information & communication | 2025 |
| Ukamhs Ltd | Arts & recreation | 2021 |
| Ulstel Limited | Information & communication | 2009 |
| Ultimate Filtration Solutions Limited | Manufacturing | 2024 |
| Ultimate Posh Wash Ltd | Administrative & support | 2020 |
| Umberry Limited | Manufacturing | 2024 |
| Umbhost Limited | Information & communication | 2021 |
| Umbrella Audio Limited | Manufacturing | 2024 |
| UMS Construction Ltd | Construction | 2021 |
| Unaegbu Ltd | Administrative & support | 2024 |
| Unaeon Limited | Information & communication | 2023 |
| Uniaccess (UK) Limited | Manufacturing | 2012 |
| Unique Crown Care Limited | Health & social care | 2023 |
| Unique Property Group (UPG) Ltd | Real estate | 2009 |
| Unique Tattoos Limited | Arts & recreation | 2016 |
| Unit 4 North Weald Limited | Other services | 1984 |
| Unite Marketing & Promotions Limited | Administrative & support | 2008 |
| Unity Boutique Living Limited | Construction | 2018 |
| Unity Fire Ltd | Construction | 2025 |
| Unity Foreign Exchange Limited | Financial services | 2022 |
| Unity Rail Limited | Professional & scientific | 2018 |
| Unity Support Ltd | Real estate | 2026 |
| Unitypmc Ltd | Professional & scientific | 2024 |
| Universal Builders (Portsmouth) Limited | Construction | 2015 |
| Universal Care Agency Ltd | Health & social care | 2009 |
| Universal Packing Specialists Limited | Administrative & support | 1985 |
| Universal Vehicle Services (Horndean) Limited | Wholesale & retail | 2007 |
| Unstoppable Learning Ltd | Education | 2024 |
| Untapped Services (Europe) Limited | Information & communication | 2022 |
| Untapped Services Limited | Information & communication | 2020 |
| UP Track Limited | Construction | 2018 |
| UPC Holdings Ltd | Financial services | 2016 |
| Upfront Property Ltd | Real estate | 2021 |
| UPG Aldershot Ltd | Construction | 2025 |
| UPG Construction Ltd | Construction | 2025 |
| UPG Westminster Ltd | Construction | 2023 |
| Upholstered by Hewett Ltd | Manufacturing | 2026 |
| Uppercutz Barbers Ltd | Other services | 2024 |
| UPS Holdco Limited | Financial services | 2019 |
| Urban Front Enterprises Limited | Administrative & support | 2025 |
| Urban GAS Plumbing & Heating Ltd | Construction | 2025 |
| Urban Locker (Paterson Court) Ltd | Real estate | 2010 |
| Urban Locker (Skinner Street) Ltd | Real estate | 2025 |
| Urban Locker Theseus Ltd | Real estate | 2014 |
| Urban Oasis Rentals Limited | Real estate | 2023 |
| Urban OG Ltd | Real estate | 2026 |
| Urban Paws Collective Limited | Wholesale & retail | 2025 |
| Urban Print Pals Ltd | Wholesale & retail | 2025 |
| Urfa Consulting Ltd | Professional & scientific | 2024 |
| Us.Man.Dere Ltd | Other services | 2023 |
| UTS Plastering Ltd | Construction | 2025 |
| Uzima Care Limited | Health & social care | 2020 |
| V & R Cooling Services Ltd | Administrative & support | 2022 |
| V & R Investments Ltd | Real estate | 2019 |
| V a Education Services Ltd | Information & communication | 2025 |
| V P R Construction Ltd | Construction | 2016 |
| V&V Property Ltd | Construction | 2023 |
| Vaeta Limited | Professional & scientific | 2006 |
| Valente Insights Limited | Professional & scientific | 2025 |
| Valeo Business Solutions Ltd | Professional & scientific | 2020 |
| Validcare (PVT) Limited | Health & social care | 2016 |
| Valley Jack Carpentry Limited | Construction | 2006 |
| Valo Optimise Ltd | Information & communication | 2026 |
| Valqual Ltd | Other services | 2025 |
| Valtry Limited | Information & communication | 2025 |
| VAM Homes Ltd | Construction | 2023 |
| Vamos Property Ltd | Real estate | 2015 |
| Van Wijk Associates Limited | Professional & scientific | 2008 |
| Vanalytical Consultants Limited | Administrative & support | 2019 |
| Vanarb Limited | Construction | 2020 |
| Vanguard Construction & Developments Ltd | Construction | 2024 |
| Vanilla Beauty Lounge Limited | Other services | 2013 |
| Vanilla Hairdressing Lounge Limited | Other services | 2014 |
| Vannos Ltd | Professional & scientific | 2018 |
| Vantech Mercedes Limited | Wholesale & retail | 2024 |
| Vanverso Conversions Limited | Manufacturing | 2023 |
| Varatek Limited | Construction | 2014 |
| Variclad Systems Ltd | Construction | 2017 |
| Varleydigital Ltd | Information & communication | 2021 |
| Vass Venn Ltd | Construction | 2021 |