| Sbrn Global Trade Limited | Manufacturing | 2024 |
| SC Accounting Limited | Professional & scientific | 2023 |
| SC Dental Studio Ltd | Health & social care | 2017 |
| SC Events Ltd | Arts & recreation | 2022 |
| SCA Building Contractors Limited | Construction | 2020 |
| Scaffold City Ltd | Construction | 2020 |
| Scaffolding Design Services Limited | Construction | 2014 |
| Scallywag Farm Ltd | Real estate | 2020 |
| Scand Invest Ltd | Professional & scientific | 2018 |
| Sced Medical Services Ltd | Health & social care | 2017 |
| Scent Galerie Ltd | Wholesale & retail | 2026 |
| School Green Road Limited | Real estate | 2014 |
| School Resource Management Limited | Education | 2024 |
| Scissorworx Ltd | Administrative & support | 2017 |
| Scitech PMO Ltd | Professional & scientific | 2025 |
| SCM Same Day Logistics Ltd | Transport & logistics | 2024 |
| Scope Innovations Limited | Water & waste | 2022 |
| Scotland Farm (Hambledon) Limited | Professional & scientific | 1964 |
| Scott Kimber Bloodstock Limited | Agriculture & fishing | 2015 |
| Scott-Drummond Holdings Limited | Financial services | 2016 |
| Scour Protection Limited | Construction | 2005 |
| Scour Protection Mats Limited | Manufacturing | 2018 |
| Scrap My Car Hampshire Limited | Wholesale & retail | 2019 |
| Scratch 72 Ltd | Wholesale & retail | 2017 |
| Scribespire Limited | Information & communication | 2023 |
| Scrum Operations Solutions Ltd | Professional & scientific | 2020 |
| Scrybe Limited | Information & communication | 2023 |
| SCT (Leopold) Ltd | Hospitality | 2022 |
| SD Clean Ltd | Administrative & support | 2026 |
| SDB (Southern) Ltd | Professional & scientific | 2014 |
| SDB South Coast Ltd | Construction | 2024 |
| Sdhac Limited | Energy | 2018 |
| SDJ YFB Limited | Construction | 2022 |
| SDL Aesthetics Ltd | Other services | 2018 |
| SDL South Coast Drylining Limited | Construction | 2024 |
| Sea Sonography Ltd | Health & social care | 2022 |
| Seabourne Solutions Limited | Professional & scientific | 2008 |
| Seal Associates (CIM) Limited | Construction | 1994 |
| Seal Sustainability Ltd | Information & communication | 2026 |
| Sealls Groundwork Services Limited | Construction | 2016 |
| Sealtek Limited | Financial services | 2025 |
| Seamless Build Solutions Ltd | Construction | 2025 |
| Seamless Media Limited | Professional & scientific | 2008 |
| Sean Boyett Limited | Professional & scientific | 2015 |
| Sean William Hoskins Ltd | Transport & logistics | 2025 |
| Seaview Car Storage Limited | Transport & logistics | 2021 |
| Seaview Cars Limited | Wholesale & retail | 2008 |
| Seaview Rental Limited | Real estate | 2021 |
| Sebastian Robert Afshar Limited | Extraterritorial | 2022 |
| SEC Property Care Ltd | Construction | 2025 |
| Sechel Therapeutics Limited | Health & social care | 2015 |
| Second Verse A&R Consultancy Ltd | Information & communication | 2025 |
| Secure Glazing & Installations Ltd | Construction | 2021 |
| Secure Scaffolding Services Limited | Construction | 2017 |
| Securechange Services Limited | Real estate | 1993 |
| Securit Locks & Keys Limited | Other services | 2018 |
| SEE Change Happen Ltd | Other services | 2021 |
| SEE Facades Fabrication Ltd | Professional & scientific | 2023 |
| SEE Surveying Limited | Professional & scientific | 2016 |
| Seismic Sewage Systems Limited | Other services | 2009 |
| Seitis Ltd | Information & communication | 2017 |
| Selad Investments Limited | Real estate | 2019 |
| Selfcharge Limited | Construction | 2019 |
| Sellingeverythang Ltd | Wholesale & retail | 2026 |
| Semicast Research Limited | Professional & scientific | 2009 |
| Sempre Property Ltd | Construction | 2022 |
| Sensibly Made Ltd | Wholesale & retail | 2022 |
| Sentha Property Ltd | Real estate | 2023 |
| Sentient Care Group Limited | Health & social care | 2022 |
| Seren Slimmers Limited | Health & social care | 2021 |
| Serenity Homes & Property Services Limited | Real estate | 2025 |
| Sero Aesthetics Ltd | Professional & scientific | 2018 |
| Servisa Limited | Financial services | 2023 |
| SET in Stone (Southern) Ltd | Construction | 2015 |
| Sets Developments Ltd | Construction | 2021 |
| Setsquare Creative Limited | Administrative & support | 2020 |
| Setsquare Global Limited | Administrative & support | 2023 |
| SEV E&I Ltd | Construction | 2018 |
| SF Facilities Management Limited | Manufacturing | 2017 |
| SFR Enterprises Limited | Financial services | 2017 |
| SFX Trade Ltd | Construction | 2006 |
| SG Hellyer Ltd | Construction | 2021 |
| SGC Plastering Southern Ltd | Construction | 2026 |
| SGE Phoenix Ltd | Construction | 2015 |
| SGL Keystone Ltd | Real estate | 2025 |
| Shades Extensions Limited | Other services | 2025 |
| Shado Tech Limited | Professional & scientific | 2019 |
| Shaikh Plumbing Limited | Construction | 2025 |
| Shalford Consultants Ltd | Professional & scientific | 2024 |
| Shambles Removals Limited | Transport & logistics | 2022 |
| Shanay Technologies Limited | Information & communication | 2019 |
| Sharpe-Stone Property Group Ltd | Real estate | 2023 |
| Shawline Investments Limited | Real estate | 2024 |
| Shca Limited | Professional & scientific | 2018 |
| Shearwater Mortgages Ltd | Financial services | 2019 |
| Shedsmiths Ltd | Manufacturing | 2024 |
| Sheepwash Farm Ltd | Agriculture & fishing | 2023 |
| Shelagh Ryan Limited | Hospitality | 2007 |
| Shellard Services Ltd | Construction | 2025 |
| Sherborne Double Glazing Repairs Limited | Construction | 2023 |