| Elser Property Developments Limited | Real estate | 2025 |
| Elston HR Ltd | Administrative & support | 2020 |
| Elsy & Gibbons,Limited | Construction | 1919 |
| EM & I (Derby) Limited | Construction | 2009 |
| EM10 Express Ltd | Wholesale & retail | 2025 |
| Emanuela Sanseverino Ltd | Information & communication | 2025 |
| Ember & Oak Ltd | Wholesale & retail | 2024 |
| Ember Construction Ltd | Construction | 2023 |
| Embrace Property Investments Limited | Real estate | 2019 |
| EMC2 Talent Solutions Ltd | Administrative & support | 2020 |
| Emdhdh Ltd | Other services | 2026 |
| Emediately Ltd | Information & communication | 2022 |
| Emergency Vehicle Products Limited | Wholesale & retail | 2014 |
| Emerix Global Limited | Financial services | 2021 |
| Emir Ozan Ltd | Hospitality | 2020 |
| EMJ Winter Investments Ltd | Real estate | 2025 |
| Emma Thomas Eventing Ltd | Agriculture & fishing | 2024 |
| Emmaljunga Limited | Manufacturing | 2000 |
| Emmaso Ltd | Wholesale & retail | 2024 |
| Emmbrook Court Limited | Health & social care | 1989 |
| Emmi Technologies Limited | Wholesale & retail | 2023 |
| Emobility Holdings Ltd | Manufacturing | 2026 |
| Emobility Technology Ltd | Manufacturing | 2026 |
| Empac Limited | Professional & scientific | 1992 |
| Emperors Mobile Delivery Limited | Transport & logistics | 2025 |
| Employment Matters Consulting Limited | Administrative & support | 2004 |
| Emscote Power Services Ltd | Professional & scientific | 2024 |
| Emscote Pre-School Ltd | Education | 2008 |
| Emscote Properties Limited | Real estate | 2025 |
| EN Route Design & Engineering Ltd | Professional & scientific | 2024 |
| Enabling HR Ltd | Professional & scientific | 2010 |
| Enamel Copenhagen Limited | Manufacturing | 2024 |
| Enera Limited | Information & communication | 2000 |
| Energold Drilling (Emea) Limited | Mining & quarrying | 2012 |
| Energy Metrics Monitoring and Management UK Limited | Professional & scientific | 2022 |
| Enesbe Limited | Wholesale & retail | 2025 |
| Enfuce UK Ltd | Information & communication | 2022 |
| Engel U.K. Limited | Manufacturing | 1964 |
| Engicorp LLP | | 2012 |
| Engineered Innovation Limited | Manufacturing | 2018 |
| Engineered Insanity Limited | Professional & scientific | 2018 |
| Engineered Limited | Professional & scientific | 2020 |
| Ensco 1772 Limited | Professional & scientific | 2021 |
| Ensco 807 Limited | Real estate | 2010 |
| Ensco 835 Limited | Real estate | 2011 |
| Ensoaura Limited | Hospitality | 2023 |
| Enterprise Accounts Ltd | Professional & scientific | 2010 |
| Enterprise Integration Experts Ltd | Information & communication | 2024 |
| Ento Energy Limited | Information & communication | 2023 |
| Entrepreneural Limited | Other services | 2024 |
| Environmental Refrigerants Ltd | Professional & scientific | 2023 |
| Envisage Advisory Ltd | Professional & scientific | 2026 |
| EOS Worldwide UK, Ltd | Education | 2019 |
| Eotr Solutions Limited | Professional & scientific | 2004 |
| EPG Land Limited | Real estate | 2025 |
| Epic Dens Limited | Administrative & support | 2022 |
| Epoch Protect Limited | Real estate | 2023 |
| Equestrian Pro Media Ltd | Information & communication | 2014 |
| Equinell Sports Massage Ltd | Agriculture & fishing | 2025 |
| Equinox Business Solutions Limited | Administrative & support | 2025 |
| Equipt Consulting Ltd | Professional & scientific | 2022 |
| Erampo Limited | Other services | 2023 |
| Eranovus Ltd | Information & communication | 2020 |
| Erebor Holdings Ltd | Information & communication | 2021 |
| Erin Byrne Photography Limited | Professional & scientific | 2016 |
| Errington Springall Defence Limited | Public administration | 2024 |
| ESL Midlands Limited | Administrative & support | 2020 |
| Espada Sports Limited | Education | 2025 |
| Essential Property Group Ltd | Real estate | 2023 |
| Essex Auto Group Ltd. | Financial services | 1989 |
| Essoldo Court Management Company Limited | Extraterritorial | 2016 |
| Estateaide Consulting Ltd | Professional & scientific | 2024 |
| Estefan's Design Limited | Professional & scientific | 2018 |
| Etelligence Consulting Limited | Information & communication | 2010 |
| ETG Bridgeport UK Limited | Wholesale & retail | 2013 |
| Etools Limited | Information & communication | 2002 |
| Eunus Limited | Professional & scientific | 2005 |
| Euro Motaparts LLP | | 2010 |
| Eurocres Consulting Limited | Professional & scientific | 2005 |
| European Automation Intelligence Limited | Information & communication | 2019 |
| European Business Solutions Ltd | Professional & scientific | 2002 |
| European Veterinary Supplies Limited | Wholesale & retail | 1995 |
| Eurotech Industries Limited | Manufacturing | 1994 |
| EV Insights Ltd | Information & communication | 2024 |
| Ev-Aaa Consulting Limited | Professional & scientific | 2026 |
| Eventia Ltd | Other services | 1994 |
| Events EUK Limited | Administrative & support | 2016 |
| Everamour Global Ltd | Wholesale & retail | 2025 |
| Everamour Ltd | Wholesale & retail | 2025 |
| Evia Tech Limited | Transport & logistics | 2024 |
| Evoluter Holdings Limited | Financial services | 2025 |
| Evolve Growing Solutions Ltd | Professional & scientific | 2012 |
| Evondos Ltd | Manufacturing | 2024 |
| Evoque Security Limited | Administrative & support | 2018 |
| Evriholder Limited | Wholesale & retail | 2025 |
| Ewhitethorn Ltd | Professional & scientific | 2026 |
| EX Enterprises Ltd | Wholesale & retail | 2023 |
| Exam Support Services Limited | Education | 2017 |
| Exams Officer Training Limited | Education | 2016 |
| Excalibur Project Management Ltd | Extraterritorial | 2013 |