| DTE Active Limited | Wholesale & retail | 2023 |
| Dual Meter Systems Limited | Energy | 2018 |
| Dunraven D Residents Limited | Households | 2000 |
| Durham Drums Ltd. | Arts & recreation | 2018 |
| DW and MW Property Management Ltd | Real estate | 2025 |
| Dycademy Limited | Professional & scientific | 2015 |
| Dyers Developments (NO.1) Ltd | Construction | 2025 |
| Dyers Developments Ltd | Construction | 2025 |
| Dyers Group Ltd | Construction | 2025 |
| Dyers Home Build Ltd | Construction | 2025 |
| Dynamic Network Systems Ltd | Construction | 2023 |
| Dzined Ltd | Information & communication | 2006 |
| E B C Plumbing and Heating Limited | Construction | 2014 |
| E Constance Ltd | Wholesale & retail | 2024 |
| E14 Consultants Ltd | Professional & scientific | 2016 |
| Eacs Limited | Information & communication | 1994 |
| Eagle Electrical Solutions Limited | Construction | 2018 |
| Eardley Advisory Limited | Professional & scientific | 2022 |
| Early Bird and Night OWL Childcare Ltd | Health & social care | 2022 |
| Early Dawn Limited | Financial services | 2011 |
| Earlylinks Limited | Administrative & support | 2014 |
| East Hertfordshire Football Ltd | Arts & recreation | 2024 |
| East Herts Catering Equipment Ltd | Manufacturing | 2021 |
| East Herts Heating Ltd | Construction | 2019 |
| East Herts PA & DJ Hire Ltd | Arts & recreation | 2025 |
| Easytruck Ltd | Agriculture & fishing | 2011 |
| EAT & More London Liverpool ST. Ltd | Manufacturing | 2021 |
| EAT Snapp Ltd | Manufacturing | 2025 |
| Eco Capital Limited | Arts & recreation | 2025 |
| Eco Pro Construction Ltd | Construction | 2025 |
| Eco Project Long Lane Limited | Arts & recreation | 2026 |
| Eco Tree Care & Conservation Limited | Agriculture & fishing | 2007 |
| Eco Worldwide Ltd | Water & waste | 2011 |
| Eco-Smartgas Heating & Plumbing Ltd | Construction | 2006 |
| Ecoheat Solutions Limited | Construction | 2013 |
| Edem Design and Build Ltd | Construction | 2022 |
| Edgecoms Consulting Ltd | Information & communication | 2023 |
| Educan International Limited | Education | 2016 |
| Edviron Environmental Limited | Water & waste | 2011 |
| Edviron Limited | Water & waste | 2011 |
| Edviron Plant Hire Limited | Water & waste | 2011 |
| EG Build Ltd | Construction | 2010 |
| EGE Furniture Limited | Wholesale & retail | 2025 |
| Eggenton Removals & Storage Ltd | Transport & logistics | 2021 |
| Eintegrity E-Learning Community Interest Company | Information & communication | 2007 |
| Elecspec Limited | Construction | 2009 |
| Electrical Certification Ltd | Construction | 2025 |
| Electro Tech Installations Limited | Construction | 2006 |
| Electroculture Limited | Extraterritorial | 2024 |
| Electronic & General Services Limited | Wholesale & retail | 1976 |
| Electronic & General Systems Limited | Real estate | 1991 |
| Elegance Dental Limited | Health & social care | 2018 |
| Elevate Media Management Ltd | Professional & scientific | 2024 |
| Elite Installation Services Limited | Energy | 2001 |
| Elite Movers Ltd | Transport & logistics | 2025 |
| Elizabeth ANN Hair Studio Limited | Other services | 2019 |
| Elvirio AI Ltd | Information & communication | 2026 |
| Elysee Wellness Ltd | Extraterritorial | 2015 |
| Emat Trading Limited | Wholesale & retail | 2003 |
| Embrace Lives Limited | Administrative & support | 2020 |
| Emean Ltd | Construction | 2021 |
| EMG Investigations Limited | Administrative & support | 2009 |
| Emi-Tech Limited | Construction | 2017 |
| Emice 27 Ltd | Hospitality | 2025 |
| Emma Herring Ltd | Information & communication | 2003 |
| Emma Siddons Limited | Real estate | 2025 |
| Emotech Design Limited | Administrative & support | 2013 |
| Emotion Smart Mobility Limited | Administrative & support | 2025 |
| Emotivo Limited | Professional & scientific | 2004 |
| Empire 1 Ltd | Real estate | 2019 |
| EN8 Construction Ltd | Construction | 2015 |
| EN8 Homes Ltd | Construction | 2015 |
| Enable Housing Group Limited | Financial services | 2026 |
| Encasa Property Management Limited | Households | 2020 |
| Encore Media Limited | Information & communication | 2013 |
| Enfield Group Holdings Limited | Financial services | 2024 |
| Enfield Safety Limited | Wholesale & retail | 2021 |
| Engineshed Limited | Professional & scientific | 2011 |
| Engiprop Group Ltd | Construction | 2014 |
| Enigma Build Limited | Construction | 2022 |
| Enigma Escape Rooms Ltd | Arts & recreation | 2024 |
| Enigma Mechanical Ltd | Construction | 2020 |
| Enviro EV Ltd | Professional & scientific | 2025 |
| Enzonic Studios Ltd | Information & communication | 2023 |
| Epistle of Joy Ltd | Information & communication | 2022 |
| EPJ Consulting Ltd | Professional & scientific | 2024 |
| Epoints Rewards Limited | Information & communication | 2010 |
| Epping Green Properties Ltd | Construction | 2024 |
| Eri-An Consulting Ltd | Professional & scientific | 2019 |
| Ermines Cafe & Stores Ltd | Hospitality | 2020 |
| Esencia Coaching Ltd | Professional & scientific | 2020 |
| Eskdale Freehold Limited | Households | 2004 |
| Espria Limited | Information & communication | 1995 |
| Essem Holdings Ltd | Real estate | 2020 |
| Etoile Lake Limited | Agriculture & fishing | 2001 |
| Euro IT Horizon Ltd | Information & communication | 2016 |
| European Flavours & Fragrances PLC | Manufacturing | 1981 |
| European Fragrances Limited | Extraterritorial | 1990 |
| Evan Joyce (Consulting) Limited | Information & communication | 1991 |
| Evented Limited | Information & communication | 2026 |