| EJB Group Ltd | Financial services | 2019 |
| EJB UK Developments Ltd. | Professional & scientific | 2019 |
| EL WIN Motorsport Ltd | Wholesale & retail | 2023 |
| El-Masri Associates Limited | Professional & scientific | 2007 |
| Elastrin Therapeutics Limited | Professional & scientific | 2022 |
| Elbir Constructions Ltd | Construction | 2021 |
| Elite Cloud Host Ltd | Information & communication | 2020 |
| ELO Media Ltd. | Professional & scientific | 2012 |
| EM Money Ltd | Education | 2022 |
| Emma Courtney Consulting Limited | Professional & scientific | 2024 |
| Enderby Property Management Ltd | Real estate | 2003 |
| Energy and Empathy LLP | | 2022 |
| Engine House Fitness Limited | Arts & recreation | 2018 |
| ENQUIR3 Ltd | Professional & scientific | 2014 |
| Epic Cabins Development Management Limited | Construction | 2026 |
| Epic Cabins Limited | Professional & scientific | 2025 |
| Epicurus Holdings Ltd | Hospitality | 2023 |
| Epona Equine Limited | Professional & scientific | 2014 |
| Equus Works Design Limited | Extraterritorial | 2015 |
| Equus Works Limited | Construction | 2015 |
| Espina Chiropractic Ltd. | Health & social care | 2014 |
| Essence Interiors Ltd | Professional & scientific | 2025 |
| Esskae Properties Ltd | Hospitality | 2018 |
| Estatesprings Ltd | Administrative & support | 2026 |
| ESU1 Ltd | Professional & scientific | 2011 |
| Eternity Media Limited | Information & communication | 2023 |
| Ethica Resolve Limited | Financial services | 2019 |
| Eudaimonia Psychotherapy & Counselling Ltd | Health & social care | 2026 |
| Evenstone Limited | Professional & scientific | 2023 |
| Everyday Bunny Ltd | Wholesale & retail | 2026 |
| Evolve Brand Sport Limited | Wholesale & retail | 2022 |
| Examinex Limited | Administrative & support | 2021 |
| Execute Marketing Limited | Other services | 2007 |
| Executive Advice Limited | Professional & scientific | 2007 |
| Experience Sport UK Limited | Wholesale & retail | 2024 |
| Expert Networking Group Limited | Information & communication | 1999 |
| Exphand Prosthetics Limited | Manufacturing | 2018 |
| Ezeitgeist Ltd | Information & communication | 2018 |
| Fahamu Limited | Information & communication | 2001 |
| Fahamu Trust | Information & communication | 2010 |
| Fair Trade Matters Limited | Professional & scientific | 1998 |
| Fairmile Accounting Ltd | Professional & scientific | 2018 |
| Falcon Transport Group Ltd | Transport & logistics | 2025 |
| Farmcrop Consultants Limited | Agriculture & fishing | 1979 |
| Farquharson Grant Limited | Administrative & support | 2009 |
| Farsho Consultancy Ltd | Other services | 2025 |
| Fast 1ONE LLP | | 2010 |
| Faulty Towers the Dining Experience Limited | Arts & recreation | 2011 |
| Fawley House Stud Limited | Agriculture & fishing | 2006 |
| FCL Fire Ltd | Extraterritorial | 2024 |
| Ferrante Global Limited | Professional & scientific | 2025 |
| Field & Co Interior Design Ltd | Professional & scientific | 2015 |
| Field Barn Holdings Limited | Financial services | 2025 |
| Fieldview (Oxfordshire) Ltd | Professional & scientific | 2018 |
| Figurati Collective Ltd | Administrative & support | 2021 |
| Fijian BBQ UK Ltd | Extraterritorial | 2022 |
| Finally Flooring Limited | Construction | 2024 |
| Finchale Blue Holdings Limited | Professional & scientific | 2018 |
| Finn Equestrian LLP | | 2021 |
| Finn Global Healthcare Recruitment Ltd | Health & social care | 2022 |
| Finn Home Care Ltd | Health & social care | 2019 |
| Fire for Effect Media Limited | Information & communication | 2017 |
| Fireclass Ltd | Public administration | 2020 |
| First Rate Logistics Limited | Transport & logistics | 2022 |
| Fishing MAN Ltd | Hospitality | 2026 |
| FIX & Clear Ltd | Construction | 2025 |
| Flec Elec Ltd | Construction | 2018 |
| Flint Change Limited | Professional & scientific | 2014 |
| Flon Bells Ltd | Information & communication | 2017 |
| Flon Properties Limited | Real estate | 2025 |
| Florence Legacy LLP | | 2024 |
| FLY Throw Ltd | Wholesale & retail | 2025 |
| FM Hair Design Limited | Other services | 2006 |
| Ford and Kent Gardens Ltd | Administrative & support | 2023 |
| Forensic Access Limited | Professional & scientific | 2004 |
| Forensic Testing Service Ltd | Health & social care | 2009 |
| Fortune Real Estate Investments Limited | Real estate | 2007 |
| Four Derbies Limited | Agriculture & fishing | 2024 |
| Fourtech UK Solutions Limited | Manufacturing | 2024 |
| Fowell Engineering Ltd | Manufacturing | 2022 |
| FOX & Co. Financial Management Limited | Financial services | 2001 |
| FOX & Furb Ltd | Manufacturing | 2021 |
| Foxwell Consult & Design Ltd | Professional & scientific | 2015 |
| Fragrance and Gifts Limited | Wholesale & retail | 1997 |
| Fran Physio and Beauty Ltd | Health & social care | 2024 |
| Frazer's of Oxford Limited | Households | 2009 |
| Freelearners Community Interest Company | Education | 2018 |
| Fresh Property Developments Ltd | Real estate | 2015 |
| Freshblue Ltd | Hospitality | 2025 |
| Freshview Energy Ltd | Energy | 2022 |
| Freudenfeld Design Ltd | Information & communication | 2019 |
| Fulfilled Investments Limited | Real estate | 1993 |
| Funky Whatnot Limited | Manufacturing | 2014 |
| Furniture for Functions Limited | Manufacturing | 2007 |
| Fusion Pharma Limited | Professional & scientific | 2013 |
| Future Delta Group Ltd | Professional & scientific | 2025 |
| Future Gadget Systems Ltd | Manufacturing | 2024 |
| G & M Building Contractors Limited | Construction | 2014 |
| G C S Carpentry & Joinery Limited | Construction | 2006 |
| G J Campbell Contractors Ltd | Administrative & support | 2024 |