| Docular Limited | Information & communication | 2019 |
| Doit Success System International Ltd | Administrative & support | 2014 |
| Dorchester Abbey Trading | Arts & recreation | 2005 |
| Doris & Boris Limited | Wholesale & retail | 2009 |
| Double C Limited | Wholesale & retail | 2021 |
| Douglas White & Sons Ltd | Construction | 2021 |
| Downlands Kennels Limited | Hospitality | 2012 |
| DR Eddy Ltd | Manufacturing | 2023 |
| DR Financial Resources Ltd | Professional & scientific | 2010 |
| DR Georgina Wilson Ltd | Health & social care | 2026 |
| Dragon AV Ltd | Professional & scientific | 2007 |
| Dragon Sky Limited | Other services | 2022 |
| Draingain Ltd | Water & waste | 2013 |
| Drayton ST Leonard Community PUB Ltd | Hospitality | 2017 |
| Dreamset Limited | Information & communication | 2004 |
| Dredging Research Limited | Professional & scientific | 1989 |
| Drey Homes Ltd. | Construction | 2018 |
| DRG Oxford Electrical Limited | Construction | 2020 |
| DRL Software Limited | Information & communication | 1995 |
| Drutopia Limited | Arts & recreation | 2017 |
| Ducker Farming Holding Company Limited | Agriculture & fishing | 2017 |
| Ducker Farming Limited | Agriculture & fishing | 2014 |
| Dustfairycleaningco Ltd | Administrative & support | 2021 |
| Dwem Ltd | Professional & scientific | 2024 |
| E & G Recruitment Ltd | Administrative & support | 2021 |
| E.Golfbusiness.Com Limited | Information & communication | 2000 |
| E3E Ltd. | Professional & scientific | 2013 |
| E5 Acupuncture Limited | Health & social care | 2016 |
| Eagle Lodge Developments Ltd | Construction | 2014 |
| Early Days Nursery School Limited | Education | 2011 |
| Earth Save Products Limited | Manufacturing | 2008 |
| Easton’S Autos Ltd | Wholesale & retail | 2025 |
| EBE Broadcast Services Limited | Information & communication | 2024 |
| ECI Nuclear Applications Consultants Ltd | Other services | 2017 |
| Eco-Developments Ltd | Real estate | 2009 |
| Ecoscape Supplies Ltd | Wholesale & retail | 2025 |
| EEW Consulting Ltd | Professional & scientific | 2023 |
| Ekatwins Limited | Arts & recreation | 2025 |
| Elesa Estates Ltd | Real estate | 1982 |
| Elesa Limited | Professional & scientific | 1983 |
| Elevate Events Ltd | Hospitality | 2018 |
| ELEVATE3 Limited | Other services | 2024 |
| Elite C & G Services Limited | Other services | 2022 |
| Elite Heating Engineers Ltd | Construction | 2023 |
| Ellsey Bellsey Ltd | Manufacturing | 2012 |
| ELM Park Tree Services Limited | Administrative & support | 2022 |
| ELM Tree Joinery Ltd | Manufacturing | 2018 |
| Eltham Developments Ltd | Construction | 2025 |
| Emarek Limited | Construction | 2012 |
| Ember Digital Media Limited | Professional & scientific | 2025 |
| EMC One Ltd | Real estate | 2026 |
| Emma Fenning Ltd | Other services | 2022 |
| Emmett of Drayton Limited | Agriculture & fishing | 1953 |
| Empress Rising Ltd | Information & communication | 2024 |
| Empyrium Digital Limited | Information & communication | 2023 |
| EMV Fisheries Ltd | Agriculture & fishing | 2017 |
| Energon Global Ltd | Administrative & support | 2013 |
| Engdia Ltd | Information & communication | 2019 |
| English Courtyard Association Limited(The) | Administrative & support | 1979 |
| Engolf Ltd | Arts & recreation | 2019 |
| Enigma Collective Ltd | Education | 2023 |
| Ennea Limited | Information & communication | 2025 |
| Environmental Building Services Limited | Construction | 2007 |
| Enzian Services Limited | Administrative & support | 2010 |
| Epic Maths Ltd | Education | 2025 |
| EPS Oxford Limited | Arts & recreation | 2014 |
| Equiniti Equine Ltd | Agriculture & fishing | 2018 |
| Equitable Energy Ltd | Professional & scientific | 2026 |
| ESI Building & Property Services Limited | Construction | 2025 |
| Esme James Group Ltd | Information & communication | 2022 |
| European Compressed Air Services Limited | Extraterritorial | 2012 |
| European Transport Solutions Limited | Wholesale & retail | 2011 |
| Evans Moore Capital Ltd | Real estate | 2025 |
| Evene Limited | Manufacturing | 2024 |
| Evenground (UK) Limited | Construction | 2026 |
| Everyday Parts Ltd | Administrative & support | 2026 |
| Ewelme Dog Training Academy Ltd | Other services | 2026 |
| Ewelme Sporting Limited | Arts & recreation | 2023 |
| Express Timber Ltd | Wholesale & retail | 2024 |
| Ez-Runner Systems Limited | Information & communication | 2009 |
| F.H.Rowse Limited | Professional & scientific | 1919 |
| Fabage Ltd | Hospitality | 2024 |
| Faimosai Ltd | Information & communication | 2026 |
| Fairbank Homes Limited | Real estate | 2021 |
| Fairbank Software Limited | Information & communication | 2015 |
| Fairlight Properties Limited | Real estate | 2002 |
| Falcon Chase International Limited | Administrative & support | 2019 |
| Fallingcow Ltd | Wholesale & retail | 2026 |
| Fanstone Fitness Ltd | Arts & recreation | 2011 |
| Faraday Watts Electrical Limited | Construction | 2024 |
| Farebrother Services Ltd | Information & communication | 2022 |
| Farrant Farming Limited | Agriculture & fishing | 2013 |
| Fathers Construction Ltd | Construction | 2021 |
| Fawley Farms Limited | Agriculture & fishing | 2000 |
| Fawleybridge Investments (Green Road Reading) LLP | | 2011 |
| Fawleybridge Student Living LLP | | 2013 |
| Feet First Shoe Repair Limited | Wholesale & retail | 2002 |
| Felicity Ashley Consulting Ltd | Other services | 2023 |
| Feline Clinical Research Group Ltd | Professional & scientific | 2025 |
| Ferry Way Consultancy Limited | Professional & scientific | 2024 |