| Adventure Gear Co Ltd | Manufacturing | 2024 |
| AF Clothing Limited | Manufacturing | 2014 |
| Airobot Dynamics Limited | Information & communication | 2021 |
| Angus Bremner Ltd | Professional & scientific | 2003 |
| Apex Forestry Ltd | Agriculture & fishing | 2018 |
| Art Stone Concrete Ltd | Manufacturing | 2025 |
| Ballantynes of Walkerburn Limited | Manufacturing | 1990 |
| Big Wild Life Limited | Education | 2020 |
| Borders Elite Limited | Construction | 2024 |
| Borders Task Busters Ltd | Construction | 2025 |
| Braveheart Consulting Limited | Professional & scientific | 2024 |
| Caberston Limited | Administrative & support | 2021 |
| Candleberry Limited | Construction | 1998 |
| Cherry Financial Limited | Financial services | 2020 |
| Cle’S Sweet Sensations Ltd | Wholesale & retail | 2024 |
| Creative Badger Ltd | Professional & scientific | 2025 |
| Curries Roofing Specialists Limited | Construction | 2024 |
| Dartolos Enterprises Ltd | Professional & scientific | 2015 |
| Daykin Property Ltd | Real estate | 2021 |
| DCB Welding Design Ltd | Construction | 2023 |
| Donkey Firewood Ltd | Agriculture & fishing | 2023 |
| E-Piphany Learning Ltd | Information & communication | 2024 |
| Eco Wolves Ltd | Agriculture & fishing | 2025 |
| Fish Fishing Tackle Ltd | Administrative & support | 2017 |
| Glendinning Groundworks Limited | Construction | 1989 |
| Highlander Banqueting Ltd | Hospitality | 2025 |
| Holylee Properties Limited | Real estate | 2004 |
| Home Basics (Tweeddale) Company Limited | Water & waste | 2002 |
| K C Logging Limited | Agriculture & fishing | 2016 |
| Kirna Consult Ltd | Professional & scientific | 2022 |
| Lemon Digital Design Limited | Manufacturing | 2007 |
| MNT Greens Limited | Administrative & support | 2021 |
| Mu.Studio (UK) Ltd | Information & communication | 2020 |
| Purpleswift UK Ltd | Information & communication | 2023 |
| R J Hall Engineering Limited | Professional & scientific | 2019 |
| Re-Gen Land & Trees Ltd | Agriculture & fishing | 2021 |
| Realistic Engineering Analysis Limited | Professional & scientific | 2013 |
| SAM Trinity Retail Limited | Wholesale & retail | 2025 |
| Sinclair Duncan Textiles Limited | Manufacturing | 2013 |
| THE WEE Scrubber Soap Co Ltd | Manufacturing | 2021 |
| Tweedvale Knitwear Limited | Manufacturing | 2013 |
| Tweedvale Properties Ltd | Real estate | 2019 |
| Unmanned Systems UK Ltd | Manufacturing | 2025 |