| Empower Menopause Ltd | Public administration | 2025 |
| Empower Wellbeing Collective CIC | Health & social care | 2025 |
| Empowered Wakefield Community Interest Company | Health & social care | 2025 |
| Empty Shops Matter CIC | Real estate | 2020 |
| Emsins Trucking Ltd | Transport & logistics | 2024 |
| EN Route Hair & Beauty Limited | Other services | 2012 |
| EN Route to Sustainability CIC | Professional & scientific | 2024 |
| Enclave Investments Ltd | Real estate | 2026 |
| Enclave Logistics Limited | Transport & logistics | 2023 |
| Encore Executive Travel Ltd | Transport & logistics | 2025 |
| Endon Roofing Limited | Construction | 2003 |
| Endor CLO Ltd | Wholesale & retail | 2024 |
| Energetika Industry Solutions Limited | Manufacturing | 2024 |
| Energize Building Solutions Limited | Construction | 2015 |
| Energy & Compliance Technology Ltd. | Information & communication | 2010 |
| Energy Insight Limited | Professional & scientific | 2008 |
| Energy Mill Gym Limited | Administrative & support | 2013 |
| Energy Services (Business Power) Ltd | Administrative & support | 2010 |
| Energy Services 24 | Professional & scientific | 2017 |
| Energyexpert Limited | Administrative & support | 2006 |
| Enfermera Clinical Ltd | Education | 2019 |
| Engaging Education | Education | 2011 |
| Engineered Service - Precision Manufacturing Ltd | Manufacturing | 2024 |
| Engineered Service Ltd | Professional & scientific | 2018 |
| Engineering Pro Ltd | Professional & scientific | 2023 |
| Enhanced Limited | Information & communication | 2010 |
| Enigma Mortgage Solutions Limited | Extraterritorial | 2023 |
| Enijes Limited | Health & social care | 2024 |
| Eniptos Enterprise Ltd. | Professional & scientific | 2009 |
| Enodatio Consulting Limited | Professional & scientific | 2013 |
| Enodatio Software Limited | Information & communication | 2013 |
| Enodatio Solutions Limited | Information & communication | 2005 |
| Enterprise 243 Ltd | Professional & scientific | 1985 |
| Entheovision Veranstaltungs Limited | Administrative & support | 2006 |
| Enviro 360 Ltd | Wholesale & retail | 2024 |
| Enviroclean Wakefield Ltd | Administrative & support | 2025 |
| Envirogood Limited | Information & communication | 2022 |
| Environment Social and Governance Compliance Ltd | Professional & scientific | 2022 |
| Enviroshine UK Ltd | Administrative & support | 2025 |
| Envirotex Ltd | Other services | 2007 |
| EP Homes Limited | Real estate | 2025 |
| EP Turbines Limited | Manufacturing | 2018 |
| Epic Range Ltd | Wholesale & retail | 2024 |
| Equate Limited | Professional & scientific | 2003 |
| Equi-Move Limited | Transport & logistics | 2012 |
| Equine Salt Therapy Co Ltd | Agriculture & fishing | 2023 |
| Equipd Ltd | Wholesale & retail | 2025 |
| Equipro Solutions Limited | Wholesale & retail | 2026 |
| ER Health Media Ltd | Extraterritorial | 2018 |
| ERA Commercials Limited | Construction | 2026 |
| Erff Ltd | Wholesale & retail | 2019 |
| Erictino Global Limited | Education | 2021 |
| Erivo Group Limited | Wholesale & retail | 2026 |
| Ermaer Limited | Construction | 2013 |
| ERZ Consultants Ltd | Administrative & support | 1998 |
| Escapable (Ridings) Limited | Other services | 2025 |
| Escapable Limited | Other services | 2021 |
| Eschwege Immobiliengesellschaft Limited | Real estate | 2010 |
| Esplanade Vale Media Limited | Information & communication | 2014 |
| ESS Assist Corporate Services Limited | Extraterritorial | 2020 |
| ESS Assist Ltd | Professional & scientific | 2016 |
| Essentia Design Limited | Professional & scientific | 2006 |
| Essential Construction Services Limited | Professional & scientific | 2014 |
| Essential Search Limited | Administrative & support | 2024 |
| Esson Property Construction Ltd | Real estate | 2024 |
| Essywellness Limited | Wholesale & retail | 2026 |
| EST Restaurants Ltd | Hospitality | 2024 |
| Estabulo (Doncaster) Ltd | Hospitality | 2023 |
| Estabulo Bar & Grill Leeds Ltd | Hospitality | 2025 |
| Estata Limited | Real estate | 2009 |
| Ester Mhike Limited | Health & social care | 2013 |
| Eternal Chique Limited | Wholesale & retail | 2024 |
| Eternalpa Ltd | Professional & scientific | 2025 |
| Eternity Photo Limited | Professional & scientific | 2011 |
| ETM Temporary Works Design Limited | Professional & scientific | 2026 |
| Eurasia Store K Limited | Wholesale & retail | 2025 |
| Eureka Display Limited | Manufacturing | 2001 |
| Euro Branding Ltd | Manufacturing | 2015 |
| Euro Fresh BD5 Limited | Wholesale & retail | 2025 |
| Euro Fresh Foods 50 Ltd | Wholesale & retail | 2023 |
| Euro Truckwash Limited | Extraterritorial | 2008 |
| European Experts Group Ltd. | Professional & scientific | 2011 |
| European Foods Agbrigg Ltd | Wholesale & retail | 2024 |
| Eurotec Support Ltd. | Construction | 2009 |
| Eurotex Limited | Water & waste | 2009 |
| Evaglades Ltd | Health & social care | 2023 |
| Evans Bathrooms Limited | Wholesale & retail | 2024 |
| Evanstaylor Aviation Sales & Charter Services Ltd | Wholesale & retail | 2022 |
| Evbiz Services Limited | Information & communication | 2016 |
| Evender Ltd | Wholesale & retail | 2025 |
| Evennow Limited | Professional & scientific | 2018 |
| Events by DUA Ltd | Hospitality | 2026 |
| Events Central C.I.C. | Arts & recreation | 2010 |
| Eventsbea Ltd | Hospitality | 2022 |
| Everett Roofing Ltd | Construction | 2026 |
| Evergold Developments Limited | Real estate | 2017 |
| Evergold Investments Limited | Real estate | 2018 |
| Evergold Property Limited | Real estate | 2016 |
| Evergreen Contractors Limited | Administrative & support | 2007 |
| Evergreen Support Group Limited | Administrative & support | 2025 |