| RS Business Solutions Limited | Professional & scientific | 2007 |
| RTS Haulage & Plant Hire Limited | Administrative & support | 2003 |
| RWB Building & Joinery Ltd | Construction | 2025 |
| S & S Carpets & Flooring Limited | Construction | 2021 |
| S a Consulting (Midlands) Ltd | Professional & scientific | 2005 |
| S J Briggs Limited | Other services | 2014 |
| S J Hopkin Ltd | Health & social care | 2019 |
| S J Mccann Transport Limited | Transport & logistics | 2000 |
| S S Scaffolding & Access Ltd | Construction | 2012 |
| S. P. Sargeant Ltd | Other services | 2014 |
| S.L.S Electrical Systems Limited | Information & communication | 2018 |
| Safer Lifestyle Ltd | Information & communication | 2019 |
| Sally Greaves Marketing Limited | Administrative & support | 2021 |
| Salon 14 Limited | Other services | 2010 |
| Salubrious Feeds Ltd | Wholesale & retail | 2018 |
| SAM & Louise Photography Limited | Professional & scientific | 2014 |
| Saplings Properties Limited | Real estate | 2024 |
| SB14 UK Sales Ltd | Wholesale & retail | 2024 |
| SBX Kitchen Installations Limited | Manufacturing | 2025 |
| Scentarea Limited | Real estate | 1998 |
| Scorpio 4 Site Services Limited | Professional & scientific | 2016 |
| Scrape Escape Limited | Wholesale & retail | 2013 |
| Secura HQ Limited | Administrative & support | 2015 |
| Sein (Staffordshire) CIC | Education | 2024 |
| Sergeant Peppers (UK) Limited | Hospitality | 2012 |
| Shape of Coffee Limited | Wholesale & retail | 2025 |
| Sharp Aviation Limited | Transport & logistics | 2024 |
| Shenton Group Limited | Financial services | 2023 |
| Shenton Holdings Limited | Real estate | 2003 |
| Sheridan Court Uttoxeter Limited | Households | 2014 |
| Showcraft Ltd | Administrative & support | 2020 |
| Sidsdeli Ltd | Hospitality | 2025 |
| Simpson Mechanical Services Limited | Manufacturing | 2010 |
| SING4ALL Limited | Administrative & support | 2026 |
| Site Support Ltd | Construction | 2018 |
| SJC Brockenhurst Limited | Real estate | 2020 |
| SJL Hire Limited | Professional & scientific | 2011 |
| Skatoo Ltd | Agriculture & fishing | 2024 |
| Skellingthorpe Sawmills Limited | Manufacturing | 2001 |
| Skin Jungle Ltd | Wholesale & retail | 2023 |
| Slack Property Group Ltd | Real estate | 2026 |
| Slake Bidco Limited | Financial services | 2019 |
| Slake Holdings Limited | Professional & scientific | 2018 |
| Slake Topco Limited | Financial services | 2021 |
| Slidesports Limited | Wholesale & retail | 2003 |
| Small Talk (Speech & Language Therapy) Ltd | Health & social care | 2007 |
| Smart Box Technology Ltd | Professional & scientific | 2022 |
| Smart Meter Box Limited | Manufacturing | 2013 |
| SMC Facilities Management Consultancy Services Limited | Professional & scientific | 2018 |
| Smithfield Carpets Limited | Wholesale & retail | 1979 |
| Snack Attack (Uttoxeter) Limited | Hospitality | 2004 |
| SOKDELIVERIES26 Limited | Transport & logistics | 2025 |
| Solar Star Power Limited | Manufacturing | 2012 |
| Solitaire Land Limited | Real estate | 2019 |
| Soph's Brownies Vendings Ltd | Wholesale & retail | 2024 |
| South Handford Development Limited | Real estate | 1998 |
| Spanish Global Properties Limited | Real estate | 2002 |
| Sparkzdirect.Co.Uk Ltd | Wholesale & retail | 2009 |
| Specialised Driver Solutions Ltd | Administrative & support | 2026 |
| Spire View Estates Ltd | Construction | 2024 |
| Sports Alive Limited | Professional & scientific | 2000 |
| Sports Circus International Limited | Administrative & support | 2011 |
| Springer Business Services Limited | Professional & scientific | 2022 |
| Srcj Construction Limited | Construction | 2021 |
| SS Terra Firma Limited | Real estate | 2024 |
| ST14 Construction Limited | Construction | 2004 |
| Staffordshire Home Surveys Limited | Professional & scientific | 2025 |
| Staffordshire Storage Limited | Other services | 2017 |
| Stags Property Services Ltd | Real estate | 2025 |
| Standard Ledger Holdings Limited | Financial services | 2024 |
| Standon Mill Limited | Real estate | 2026 |
| Starion Services Limited | Construction | 2006 |
| Steeplechase Management Company Limited | Real estate | 2002 |
| Steve Bosworth Engineering Services Limited | Professional & scientific | 2015 |
| Steve's Flooring Ltd | Construction | 2022 |
| Steven's Plastering Limited | Construction | 2015 |
| Stone Brook Audio Ltd | Information & communication | 2026 |
| Stop N Snack Ltd | Hospitality | 2014 |
| Storer-Smith Events Limited | Other services | 2005 |
| Stradale Italia Ltd | Wholesale & retail | 2019 |
| Strange Automotive Ltd | Wholesale & retail | 2021 |
| Strategy LED Ltd | Information & communication | 2021 |
| Stretton Joinery Construction Limited | Real estate | 1985 |
| Structural Steel Decking Limited | Construction | 2019 |
| STUDIO52 Pilates Ltd | Arts & recreation | 2025 |
| Superior Education Limited | Professional & scientific | 2024 |
| Supersealer UK Ltd | Manufacturing | 2013 |
| SW Building & Plastering Ltd | Construction | 2023 |
| Sweet Investments Limited | Financial services | 2019 |
| Swift Tuning Ltd | Wholesale & retail | 2024 |
| Swinson Holdings Limited | Professional & scientific | 2014 |
| Swinson Properties Ltd | Real estate | 2021 |
| Swinson Transport Limited | Transport & logistics | 2014 |
| Sylvester FUN Gym Ltd | Arts & recreation | 2006 |
| Synkronized Ltd | Administrative & support | 2022 |
| System Solutions (Midlands) Ltd | Professional & scientific | 2018 |
| Systemstec Ltd | Financial services | 2024 |
| Szymczyk Transport Ltd | Transport & logistics | 2018 |
| T & S Carpets and Flooring Limited | Wholesale & retail | 2016 |
| T J Nutt Limited | Construction | 2022 |